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It's tricky but we've done it using the Photonic Instruments Micropoint FRAP laser and collecting widefield images with a Nikon TE2000 (100x 1.4 NA). You definitely need a diffraction limited spot.
Jennifer
On 9/13/07, Elaine Kunze <[hidden email]> wrote:
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Has anyone tried FRAP in bacteia? I have a user who wants to FRAP GFP. We have an Olympus 1000 and I'm not sue we can get the spot size small enough. Hints/Comments/Discouraging words?
Elaine Kunze
Flow Cytometry and Imaging Facility
Huck Institutes of the Life Science
319 Life Sciences Building
Penn State University
University Park, PA 16802
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814-863-2762
--
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School
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