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Leica Microsystems and Microscopy & Microanalysis have organized a free
web-seminar in July that may be of interest to you:
Correlative fluorescence and (cryo-) electron microscopy
Date: 7th July 2015
Time: 11am U.S. Eastern Time
Duration: 60 minutes
http://tinyurl.com/cryoclemPresenter:
Dr. John Briggs
Group Leader & Senior Scientist
EMBL Heidelberg
Dynamic and rare events in cells can be hard to find and image by electron
microscopy. Using correlative light and electron microscopy (CLEM)
methods, it is possible to identify objects of interest based on their
fluorescent signal, and then find and image them in 3D in the transmission
electron microscope. This webinar will describe methods for high-precision
CLEM and their applications to cell biological problems.
The next challenge is to make these methods work at liquid Nitrogen
temperatures – to perform cryo-fluoresence microscopy and subsequent
cryo-electron microscopy. There are two reasons to do this. First, cryo-
fluorescence microscopy can take advantage of improved structural
preservation and improved fluorophore properties at low temperature.
Second, cryo-EM has the power to resolve very detailed structural
information on proteins. This webinar will address both the challenges and
the potential of cryo-CLEM, as well as current solutions.
Very best regards,
Lon Nelson
Sr. Director of Marketing & Commercial Analytics
Leica Microsystems – Americas
Living up to Life
(847) 317-7218
(800) 248-0123
[hidden email]
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