Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalDear All,
Just a quick note to let you know that there is an updated version of my
image analysis program available for download (free) from:
http://rct-cjs3.pdn.cam.ac.ukIt now reads Leica SP5 files, Zeiss LSM files, JPGs, BMPs and GIFs as well
as a particular variant of Video Savant files from a two photon microscope
I was using in Australia.
If you what to plot ROIs from 8-bit timeseries data, you should find my
program is quite nippy compared to the Leica software. For instance my
program is about 50 times faster than the Leica software in one particular
trial. Using the Leica software a 2.2 GB file of ours takes ~8 minutes to
produce a plot on a fast computer with the Leica software (reading direct
from the hard disk). My program, pulling the data across the network and
running on a considerably older computer takes about 10s to produce a
similar plot. Of course performance with your files/system may be different.
There is no magic involved, my program simply optimizes data transfer by
only reading the portions of the image that are actually required. Why
calculate every frame in a sequence 10,000 images long if it is to be
plotted in a 500 pixel wide box? I make no attempt to read datafiles into
memory. Zooming in to graphs is simple, drag the cursor across the graph
over the desired area or right click to zoom out. The data on the graph is
instantly and continuously available on the clipboard for pasting into
Excel.
My program is small (~700KB) will run on a 486 machine with 64MB of RAM and
still analyze a 10GB file.
Thought that you might like to know about it......
Locked
