From MDPI: "Circular Intensity Differential Scattering for Label-Free Chromatin Characterization: A Review for Optical Microscopy"

[Alberto Diaspro-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thinking about Sissi, usually on my desk...I missed the dimensioons I wanted to add...so


Dear,
this is our way towards label free microscopy. Coupling with super resolved fluorescence microscopy contributes to understand what we can learn from label-free solo signature.
We are back to study chromatin following Carlos Bustamante results in the 80s and our initali attempts early in the 90s. Back thanks to improved technologies and the chance to correlate with super resolved fluorescence microscopy.
All the best
Alby


Open access short review
https://www.mdpi.com/865122:

Circular Intensity Differential Scattering for Label-Free Chromatin Characterization: A Review for Optical Microscopy

Abstract: Circular Intensity Differential Scattering (CIDS) provides a differential measurement of the circular right and left polarized light and has been proven to be a gold standard label-free technique to study the molecular conformation of complex biopolymers, such as chromatin. In early works, it has been shown that the scattering component of the CIDS signal gives information from the long-range chiral organization on a scale down to 1/10th–1/20th of the excitation wavelength, leading to information related to the structure and orientation of biopolymers in situ at the nanoscale. In this paper, we review the typical methods and technologies employed for measuring this signal coming from complex macro-molecules ordering. Additionally, we include a general description of the experimental architectures employed for spectroscopic CIDS measurements, angular or spectral, and of the most recent advances in the field of optical imaging microscopy, allowing a visualization of the chromatin organization in situ.


-------------------------
Alberto Diaspro
Dipartimento di Fisica, UNIGE
Dipartimento di Nanofisica, IIT
https://peerj.com/Diaspro/
——————————————