Fwd: Advice on lens cleaning
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** > Hey Gabriel, > > I consulted with one of the optics experts over here and he recommends the following: > > Fill a beaker with toluene and create a rig above the beaker that will allow you to hold the tip of the objective with the oily crystals submerged in the toluene. Leave it there for a week, take it out and then re-examine the lens. Hopefully you can use standard cleaning procedures after that for any residual muck. Best of luck! > > John Oreopoulos > Staff Scientist > Spectral Applied Research > Richmond Hill, Ontario > Canada > www.spectral.ca > > On 2013-04-26, at 2:03 PM, Gabriel Lapointe <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I have a problem with a dirty objective installed on a straight Leica >> microscope. The lens is covered in what looks like oil mixed with solid >> dark green particles and the metal edge with green oily crystal. That >> system hasn't been used for a while so I’m inclined to think that immersion >> oil was left on the objective for more than a year. >> >> Have you ever seen anything like that and do you have any suggestion as to >> how I should start the cleaning? I’m afraid to use my standard cleaning >> protocol as the particles inside the oil could scratch the lens. >> >> Thanks for your help. >> *Gabriel Lapointe, M.Sc.* >> Lab Manager / Microscopy Specialist >> Concordia University, Biology Department >> 7141 Sherbrooke St. West SP 534 >> Montréal QC H4B 1R6 Canada >> Lab : (514) 848-2424 x5988 >> Office : (514) 848-2424 x3008 >> Fax : (514) 848-2881 >> Cell : (514) 278-0247 >> [hidden email] >> cmac.concordia.ca >> http://gabriellapointe.ca |
 
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JOEL B. SHEFFIELD |
Re: Advice on lens cleaning
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Wow! Toluene? We have had the experience of immersion oil crystalizing in the vial. Heating it slightly made the crystals dissolve. Perhaps a warm bath would be helpful in this case, or just warming the lens to 38 or so.. Joel On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > Hey Gabriel, > > > > I consulted with one of the optics experts over here and he recommends > the following: > > > > Fill a beaker with toluene and create a rig above the beaker that will > allow you to hold the tip of the objective with the oily crystals submerged > in the toluene. Leave it there for a week, take it out and then re-examine > the lens. Hopefully you can use standard cleaning procedures after that for > any residual muck. Best of luck! > > > > John Oreopoulos > > Staff Scientist > > Spectral Applied Research > > Richmond Hill, Ontario > > Canada > > www.spectral.ca > > > > On 2013-04-26, at 2:03 PM, Gabriel Lapointe < > [hidden email]> wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> I have a problem with a dirty objective installed on a straight Leica > >> microscope. The lens is covered in what looks like oil mixed with solid > >> dark green particles and the metal edge with green oily crystal. That > >> system hasn't been used for a while so I’m inclined to think that > immersion > >> oil was left on the objective for more than a year. > >> > >> Have you ever seen anything like that and do you have any suggestion as > to > >> how I should start the cleaning? I’m afraid to use my standard cleaning > >> protocol as the particles inside the oil could scratch the lens. > >> > >> Thanks for your help. > >> *Gabriel Lapointe, M.Sc.* > >> Lab Manager / Microscopy Specialist > >> Concordia University, Biology Department > >> 7141 Sherbrooke St. West SP 534 > >> Montréal QC H4B 1R6 Canada > >> Lab : (514) 848-2424 x5988 > >> Office : (514) 848-2424 x3008 > >> Fax : (514) 848-2881 > >> Cell : (514) 278-0247 > >> [hidden email] > >> cmac.concordia.ca > >> http://gabriellapointe.ca > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
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JOEL B. SHEFFIELD |
Re: Advice on lens cleaning
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I should add that the oil with the crystals was from Leica. Joel On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> wrote: > Wow! Toluene? We have had the experience of immersion oil crystalizing > in the vial. Heating it slightly made the crystals dissolve. Perhaps a > warm bath would be helpful in this case, or just warming the lens to 38 or > so.. > > Joel > > On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < > [hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> > Hey Gabriel, >> > >> > I consulted with one of the optics experts over here and he recommends >> the following: >> > >> > Fill a beaker with toluene and create a rig above the beaker that will >> allow you to hold the tip of the objective with the oily crystals submerged >> in the toluene. Leave it there for a week, take it out and then re-examine >> the lens. Hopefully you can use standard cleaning procedures after that for >> any residual muck. Best of luck! >> > >> > John Oreopoulos >> > Staff Scientist >> > Spectral Applied Research >> > Richmond Hill, Ontario >> > Canada >> > www.spectral.ca >> > >> > On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >> [hidden email]> wrote: >> > >> >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >> ***** >> >> >> >> I have a problem with a dirty objective installed on a straight Leica >> >> microscope. The lens is covered in what looks like oil mixed with solid >> >> dark green particles and the metal edge with green oily crystal. That >> >> system hasn't been used for a while so I’m inclined to think that >> immersion >> >> oil was left on the objective for more than a year. >> >> >> >> Have you ever seen anything like that and do you have any suggestion >> as to >> >> how I should start the cleaning? I’m afraid to use my standard cleaning >> >> protocol as the particles inside the oil could scratch the lens. >> >> >> >> Thanks for your help. >> >> *Gabriel Lapointe, M.Sc.* >> >> Lab Manager / Microscopy Specialist >> >> Concordia University, Biology Department >> >> 7141 Sherbrooke St. West SP 534 >> >> Montréal QC H4B 1R6 Canada >> >> Lab : (514) 848-2424 x5988 >> >> Office : (514) 848-2424 x3008 >> >> Fax : (514) 848-2881 >> >> Cell : (514) 278-0247 >> >> [hidden email] >> >> cmac.concordia.ca >> >> http://gabriellapointe.ca >> > > > > -- > > > Joel B. Sheffield, Ph.D > Department of Biology > Temple University > Philadelphia, PA 19122 > Voice: 215 204 8839 > e-mail: [hidden email] > URL: http://astro.temple.edu/~jbs > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** True, that. I had green crystals form in Leica oil as well! I can't remember for sure, but I think we heated it to dissolve them, or at least mobilize the oil enough that could be gently wiped off before trying any cleaner. Good luck and aloha, Tina > > I should add that the oil with the crystals was from Leica. > Joel > > > On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> wrote: > >> Wow! Toluene? We have had the experience of immersion oil crystalizing >> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >> warm bath would be helpful in this case, or just warming the lens to 38 or >> so.. >> >> Joel >> >> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >> [hidden email]> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>>> Hey Gabriel, >>>> >>>> I consulted with one of the optics experts over here and he recommends >>> the following: >>>> >>>> Fill a beaker with toluene and create a rig above the beaker that will >>> allow you to hold the tip of the objective with the oily crystals submerged >>> in the toluene. Leave it there for a week, take it out and then re-examine >>> the lens. Hopefully you can use standard cleaning procedures after that for >>> any residual muck. Best of luck! >>>> >>>> John Oreopoulos >>>> Staff Scientist >>>> Spectral Applied Research >>>> Richmond Hill, Ontario >>>> Canada >>>> www.spectral.ca >>>> >>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>> [hidden email]> wrote: >>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> I have a problem with a dirty objective installed on a straight Leica >>>>> microscope. The lens is covered in what looks like oil mixed with solid >>>>> dark green particles and the metal edge with green oily crystal. That >>>>> system hasn't been used for a while so I’m inclined to think that >>> immersion >>>>> oil was left on the objective for more than a year. >>>>> >>>>> Have you ever seen anything like that and do you have any suggestion >>> as to >>>>> how I should start the cleaning? I’m afraid to use my standard cleaning >>>>> protocol as the particles inside the oil could scratch the lens. >>>>> >>>>> Thanks for your help. >>>>> *Gabriel Lapointe, M.Sc.* >>>>> Lab Manager / Microscopy Specialist >>>>> Concordia University, Biology Department >>>>> 7141 Sherbrooke St. West SP 534 >>>>> Montréal QC H4B 1R6 Canada >>>>> Lab : (514) 848-2424 x5988 >>>>> Office : (514) 848-2424 x3008 >>>>> Fax : (514) 848-2881 >>>>> Cell : (514) 278-0247 >>>>> [hidden email] >>>>> cmac.concordia.ca >>>>> http://gabriellapointe.ca >>> >> >> >> >> -- >> >> >> Joel B. Sheffield, Ph.D >> Department of Biology >> Temple University >> Philadelphia, PA 19122 >> Voice: 215 204 8839 >> e-mail: [hidden email] >> URL: http://astro.temple.edu/~jbs >> > > > > -- > > > Joel B. Sheffield, Ph.D > Department of Biology > Temple University > Philadelphia, PA 19122 > Voice: 215 204 8839 > e-mail: [hidden email] > URL: http://astro.temple.edu/~jbs > * Tina (Weatherby) Carvalho * [hidden email] * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* **************************************************************************** |
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Matthew Nicholas-2 |
Re: Advice on lens cleaning
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I've never dealt with this problem before, so this may be off the mark, but what about using more of the oil itself to dissolve the crystals? Good luck! Matt On 4/26/13 10:47 PM, Tina Carvalho wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > True, that. I had green crystals form in Leica oil as well! I can't > remember for sure, but I think we heated it to dissolve them, or at > least mobilize the oil enough that could be gently wiped off before > trying any cleaner. > > Good luck and aloha, > Tina > >> >> I should add that the oil with the crystals was from Leica. >> Joel >> >> >> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> >> wrote: >> >>> Wow! Toluene? We have had the experience of immersion oil >>> crystalizing >>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >>> warm bath would be helpful in this case, or just warming the lens to >>> 38 or >>> so.. >>> >>> Joel >>> >>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >>> [hidden email]> wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>>> Hey Gabriel, >>>>> >>>>> I consulted with one of the optics experts over here and he >>>>> recommends >>>> the following: >>>>> >>>>> Fill a beaker with toluene and create a rig above the beaker that >>>>> will >>>> allow you to hold the tip of the objective with the oily crystals >>>> submerged >>>> in the toluene. Leave it there for a week, take it out and then >>>> re-examine >>>> the lens. Hopefully you can use standard cleaning procedures after >>>> that for >>>> any residual muck. Best of luck! >>>>> >>>>> John Oreopoulos >>>>> Staff Scientist >>>>> Spectral Applied Research >>>>> Richmond Hill, Ontario >>>>> Canada >>>>> www.spectral.ca >>>>> >>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>>> [hidden email]> wrote: >>>>> >>>>>> ***** >>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>> ***** >>>>>> >>>>>> I have a problem with a dirty objective installed on a straight >>>>>> Leica >>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>> solid >>>>>> dark green particles and the metal edge with green oily crystal. >>>>>> That >>>>>> system hasn't been used for a while so I’m inclined to think that >>>> immersion >>>>>> oil was left on the objective for more than a year. >>>>>> >>>>>> Have you ever seen anything like that and do you have any suggestion >>>> as to >>>>>> how I should start the cleaning? I’m afraid to use my standard >>>>>> cleaning >>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>> >>>>>> Thanks for your help. >>>>>> *Gabriel Lapointe, M.Sc.* >>>>>> Lab Manager / Microscopy Specialist >>>>>> Concordia University, Biology Department >>>>>> 7141 Sherbrooke St. West SP 534 >>>>>> Montréal QC H4B 1R6 Canada >>>>>> Lab : (514) 848-2424 x5988 >>>>>> Office : (514) 848-2424 x3008 >>>>>> Fax : (514) 848-2881 >>>>>> Cell : (514) 278-0247 >>>>>> [hidden email] >>>>>> cmac.concordia.ca >>>>>> http://gabriellapointe.ca >>>> >>> >>> >>> >>> -- >>> >>> >>> Joel B. Sheffield, Ph.D >>> Department of Biology >>> Temple University >>> Philadelphia, PA 19122 >>> Voice: 215 204 8839 >>> e-mail: [hidden email] >>> URL: http://astro.temple.edu/~jbs >>> >> >> >> >> -- >> >> >> Joel B. Sheffield, Ph.D >> Department of Biology >> Temple University >> Philadelphia, PA 19122 >> Voice: 215 204 8839 >> e-mail: [hidden email] >> URL: http://astro.temple.edu/~jbs >> > > **************************************************************************** > > * Tina (Weatherby) Carvalho * > [hidden email] * > * Biological Electron Microscope Facility * (808) > 956-6251 * > * University of Hawaii at Manoa * > http://www.pbrc.hawaii.edu/bemf* > **************************************************************************** -- Matthew Nicholas Medical Scientist Training Program Student Laboratory of Arne Gennerich Department of Anatomy and Structural Biology Albert Einstein College of Medicine Forchheimer Building, Room 628 1300 Morris Park Avenue Bronx, New York 10461 718.430.3446 [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The tiniest bit of (warm) distilled water in a small beaker, just enough to touch the front lens, may do the trick. And Zeiss also had a crystallising immersion oil several years ago, without green crystals though. _____________________________________ Philippe Laissue, PhD, Bioimaging Manager School of Biological Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK (0044) 01206 872246 / (0044) 07842 676 456 [hidden email] privatewww.essex.ac.uk/~plaissue On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I've never dealt with this problem before, so this may be off the mark, but > what about using more of the oil itself to dissolve the crystals? > > Good luck! > Matt > > On 4/26/13 10:47 PM, Tina Carvalho wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> True, that. I had green crystals form in Leica oil as well! I can't >> remember for sure, but I think we heated it to dissolve them, or at least >> mobilize the oil enough that could be gently wiped off before trying any >> cleaner. >> >> Good luck and aloha, >> Tina >> >>> >>> I should add that the oil with the crystals was from Leica. >>> Joel >>> >>> >>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> >>> wrote: >>> >>>> Wow! Toluene? We have had the experience of immersion oil crystalizing >>>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >>>> warm bath would be helpful in this case, or just warming the lens to 38 >>>> or >>>> so.. >>>> >>>> Joel >>>> >>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >>>> [hidden email]> wrote: >>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>>> Hey Gabriel, >>>>>> >>>>>> I consulted with one of the optics experts over here and he recommends >>>>> >>>>> the following: >>>>>> >>>>>> >>>>>> Fill a beaker with toluene and create a rig above the beaker that will >>>>> >>>>> allow you to hold the tip of the objective with the oily crystals >>>>> submerged >>>>> in the toluene. Leave it there for a week, take it out and then >>>>> re-examine >>>>> the lens. Hopefully you can use standard cleaning procedures after that >>>>> for >>>>> any residual muck. Best of luck! >>>>>> >>>>>> >>>>>> John Oreopoulos >>>>>> Staff Scientist >>>>>> Spectral Applied Research >>>>>> Richmond Hill, Ontario >>>>>> Canada >>>>>> www.spectral.ca >>>>>> >>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>>>> >>>>> [hidden email]> wrote: >>>>>> >>>>>> >>>>>>> ***** >>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>> ***** >>>>>>> >>>>>>> I have a problem with a dirty objective installed on a straight Leica >>>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>>> solid >>>>>>> dark green particles and the metal edge with green oily crystal. That >>>>>>> system hasn't been used for a while so I’m inclined to think that >>>>> >>>>> immersion >>>>>>> >>>>>>> oil was left on the objective for more than a year. >>>>>>> >>>>>>> Have you ever seen anything like that and do you have any suggestion >>>>> >>>>> as to >>>>>>> >>>>>>> how I should start the cleaning? I’m afraid to use my standard >>>>>>> cleaning >>>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>>> >>>>>>> Thanks for your help. >>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>> Lab Manager / Microscopy Specialist >>>>>>> Concordia University, Biology Department >>>>>>> 7141 Sherbrooke St. West SP 534 >>>>>>> Montréal QC H4B 1R6 Canada >>>>>>> Lab : (514) 848-2424 x5988 >>>>>>> Office : (514) 848-2424 x3008 >>>>>>> Fax : (514) 848-2881 >>>>>>> Cell : (514) 278-0247 >>>>>>> [hidden email] >>>>>>> cmac.concordia.ca >>>>>>> http://gabriellapointe.ca >>>>> >>>>> >>>> >>>> >>>> >>>> -- >>>> >>>> >>>> Joel B. Sheffield, Ph.D >>>> Department of Biology >>>> Temple University >>>> Philadelphia, PA 19122 >>>> Voice: 215 204 8839 >>>> e-mail: [hidden email] >>>> URL: http://astro.temple.edu/~jbs >>>> >>> >>> >>> >>> -- >>> >>> >>> Joel B. Sheffield, Ph.D >>> Department of Biology >>> Temple University >>> Philadelphia, PA 19122 >>> Voice: 215 204 8839 >>> e-mail: [hidden email] >>> URL: http://astro.temple.edu/~jbs >>> >> >> >> **************************************************************************** >> * Tina (Weatherby) Carvalho * [hidden email] >> * >> * Biological Electron Microscope Facility * (808) 956-6251 >> * >> * University of Hawaii at Manoa * >> http://www.pbrc.hawaii.edu/bemf* >> >> **************************************************************************** > > > -- > Matthew Nicholas > Medical Scientist Training Program Student > Laboratory of Arne Gennerich > Department of Anatomy and Structural Biology > Albert Einstein College of Medicine > Forchheimer Building, Room 628 > 1300 Morris Park Avenue > Bronx, New York 10461 > 718.430.3446 > [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Gabriel, I should stress that my toluene soaking suggestion has never been tried here in house and was only suggested as a last ditch effort to save a lens in a dire situation like the one you described. The other suggestions made already seem much "milder" and should probably be tried before the method I mentioned. Try at your own discretion!... And do let us know how it all goes afterwards. Cheers, John Oreopoulos On 2013-04-27, at 7:21 AM, phil laissue wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > The tiniest bit of (warm) distilled water in a small beaker, just > enough to touch the front lens, may do the trick. And Zeiss also had a > crystallising immersion oil several years ago, without green crystals > though. > _____________________________________ > Philippe Laissue, PhD, Bioimaging Manager > School of Biological Sciences, Room 4.17 > University of Essex, Colchester CO4 3SQ, UK > (0044) 01206 872246 / (0044) 07842 676 456 > [hidden email] > privatewww.essex.ac.uk/~plaissue > > > On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas > <[hidden email]> wrote: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I've never dealt with this problem before, so this may be off the mark, but >> what about using more of the oil itself to dissolve the crystals? >> >> Good luck! >> Matt >> >> On 4/26/13 10:47 PM, Tina Carvalho wrote: >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> True, that. I had green crystals form in Leica oil as well! I can't >>> remember for sure, but I think we heated it to dissolve them, or at least >>> mobilize the oil enough that could be gently wiped off before trying any >>> cleaner. >>> >>> Good luck and aloha, >>> Tina >>> >>>> >>>> I should add that the oil with the crystals was from Leica. >>>> Joel >>>> >>>> >>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> >>>> wrote: >>>> >>>>> Wow! Toluene? We have had the experience of immersion oil crystalizing >>>>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >>>>> warm bath would be helpful in this case, or just warming the lens to 38 >>>>> or >>>>> so.. >>>>> >>>>> Joel >>>>> >>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >>>>> [hidden email]> wrote: >>>>> >>>>>> ***** >>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>> ***** >>>>>> >>>>>>> Hey Gabriel, >>>>>>> >>>>>>> I consulted with one of the optics experts over here and he recommends >>>>>> >>>>>> the following: >>>>>>> >>>>>>> >>>>>>> Fill a beaker with toluene and create a rig above the beaker that will >>>>>> >>>>>> allow you to hold the tip of the objective with the oily crystals >>>>>> submerged >>>>>> in the toluene. Leave it there for a week, take it out and then >>>>>> re-examine >>>>>> the lens. Hopefully you can use standard cleaning procedures after that >>>>>> for >>>>>> any residual muck. Best of luck! >>>>>>> >>>>>>> >>>>>>> John Oreopoulos >>>>>>> Staff Scientist >>>>>>> Spectral Applied Research >>>>>>> Richmond Hill, Ontario >>>>>>> Canada >>>>>>> www.spectral.ca >>>>>>> >>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>>>>> >>>>>> [hidden email]> wrote: >>>>>>> >>>>>>> >>>>>>>> ***** >>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>> ***** >>>>>>>> >>>>>>>> I have a problem with a dirty objective installed on a straight Leica >>>>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>>>> solid >>>>>>>> dark green particles and the metal edge with green oily crystal. That >>>>>>>> system hasn't been used for a while so I’m inclined to think that >>>>>> >>>>>> immersion >>>>>>>> >>>>>>>> oil was left on the objective for more than a year. >>>>>>>> >>>>>>>> Have you ever seen anything like that and do you have any suggestion >>>>>> >>>>>> as to >>>>>>>> >>>>>>>> how I should start the cleaning? I’m afraid to use my standard >>>>>>>> cleaning >>>>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>>>> >>>>>>>> Thanks for your help. >>>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>>> Lab Manager / Microscopy Specialist >>>>>>>> Concordia University, Biology Department >>>>>>>> 7141 Sherbrooke St. West SP 534 >>>>>>>> Montréal QC H4B 1R6 Canada >>>>>>>> Lab : (514) 848-2424 x5988 >>>>>>>> Office : (514) 848-2424 x3008 >>>>>>>> Fax : (514) 848-2881 >>>>>>>> Cell : (514) 278-0247 >>>>>>>> [hidden email] >>>>>>>> cmac.concordia.ca >>>>>>>> http://gabriellapointe.ca >>>>>> >>>>>> >>>>> >>>>> >>>>> >>>>> -- >>>>> >>>>> >>>>> Joel B. Sheffield, Ph.D >>>>> Department of Biology >>>>> Temple University >>>>> Philadelphia, PA 19122 >>>>> Voice: 215 204 8839 >>>>> e-mail: [hidden email] >>>>> URL: http://astro.temple.edu/~jbs >>>>> >>>> >>>> >>>> >>>> -- >>>> >>>> >>>> Joel B. Sheffield, Ph.D >>>> Department of Biology >>>> Temple University >>>> Philadelphia, PA 19122 >>>> Voice: 215 204 8839 >>>> e-mail: [hidden email] >>>> URL: http://astro.temple.edu/~jbs >>>> >>> >>> >>> **************************************************************************** >>> * Tina (Weatherby) Carvalho * [hidden email] >>> * >>> * Biological Electron Microscope Facility * (808) 956-6251 >>> * >>> * University of Hawaii at Manoa * >>> http://www.pbrc.hawaii.edu/bemf* >>> >>> **************************************************************************** >> >> >> -- >> Matthew Nicholas >> Medical Scientist Training Program Student >> Laboratory of Arne Gennerich >> Department of Anatomy and Structural Biology >> Albert Einstein College of Medicine >> Forchheimer Building, Room 628 >> 1300 Morris Park Avenue >> Bronx, New York 10461 >> 718.430.3446 >> [hidden email] |
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B. Prabhakar Pandian |
Pre-cleaned Coverslips/Microscope Slides
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello Everyone, Can anyone point me to the best and cost-effective place for getting pre-cleaned coverslips and microscope slides. Regard, -Prabhakar |
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In reply to this post by John Oreopoulos
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** What John said, but maybe try a Methanol soak before resorting to toluene. I find it does a decent job of breaking up lens oil in general. I'm not sure how it will work on your crystals, but if the warm water bath doesn't work, maybe try a methanol bath? Craig On Mon, Apr 29, 2013 at 12:43 PM, John Oreopoulos < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Gabriel, > > I should stress that my toluene soaking suggestion has never been tried > here in house and was only suggested as a last ditch effort to save a lens > in a dire situation like the one you described. The other suggestions made > already seem much "milder" and should probably be tried before the method I > mentioned. Try at your own discretion!... And do let us know how it all > goes afterwards. > > Cheers, > > John Oreopoulos > > > On 2013-04-27, at 7:21 AM, phil laissue wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > The tiniest bit of (warm) distilled water in a small beaker, just > > enough to touch the front lens, may do the trick. And Zeiss also had a > > crystallising immersion oil several years ago, without green crystals > > though. > > _____________________________________ > > Philippe Laissue, PhD, Bioimaging Manager > > School of Biological Sciences, Room 4.17 > > University of Essex, Colchester CO4 3SQ, UK > > (0044) 01206 872246 / (0044) 07842 676 456 > > [hidden email] > > privatewww.essex.ac.uk/~plaissue > > > > > > On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas > > <[hidden email]> wrote: > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> I've never dealt with this problem before, so this may be off the mark, > but > >> what about using more of the oil itself to dissolve the crystals? > >> > >> Good luck! > >> Matt > >> > >> On 4/26/13 10:47 PM, Tina Carvalho wrote: > >>> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> ***** > >>> > >>> True, that. I had green crystals form in Leica oil as well! I can't > >>> remember for sure, but I think we heated it to dissolve them, or at > least > >>> mobilize the oil enough that could be gently wiped off before trying > any > >>> cleaner. > >>> > >>> Good luck and aloha, > >>> Tina > >>> > >>>> > >>>> I should add that the oil with the crystals was from Leica. > >>>> Joel > >>>> > >>>> > >>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> > >>>> wrote: > >>>> > >>>>> Wow! Toluene? We have had the experience of immersion oil > crystalizing > >>>>> in the vial. Heating it slightly made the crystals dissolve. > Perhaps a > >>>>> warm bath would be helpful in this case, or just warming the lens to > 38 > >>>>> or > >>>>> so.. > >>>>> > >>>>> Joel > >>>>> > >>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < > >>>>> [hidden email]> wrote: > >>>>> > >>>>>> ***** > >>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>> ***** > >>>>>> > >>>>>>> Hey Gabriel, > >>>>>>> > >>>>>>> I consulted with one of the optics experts over here and he > recommends > >>>>>> > >>>>>> the following: > >>>>>>> > >>>>>>> > >>>>>>> Fill a beaker with toluene and create a rig above the beaker that > will > >>>>>> > >>>>>> allow you to hold the tip of the objective with the oily crystals > >>>>>> submerged > >>>>>> in the toluene. Leave it there for a week, take it out and then > >>>>>> re-examine > >>>>>> the lens. Hopefully you can use standard cleaning procedures after > that > >>>>>> for > >>>>>> any residual muck. Best of luck! > >>>>>>> > >>>>>>> > >>>>>>> John Oreopoulos > >>>>>>> Staff Scientist > >>>>>>> Spectral Applied Research > >>>>>>> Richmond Hill, Ontario > >>>>>>> Canada > >>>>>>> www.spectral.ca > >>>>>>> > >>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < > >>>>>> > >>>>>> [hidden email]> wrote: > >>>>>>> > >>>>>>> > >>>>>>>> ***** > >>>>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>>>> ***** > >>>>>>>> > >>>>>>>> I have a problem with a dirty objective installed on a straight > Leica > >>>>>>>> microscope. The lens is covered in what looks like oil mixed with > >>>>>>>> solid > >>>>>>>> dark green particles and the metal edge with green oily crystal. > That > >>>>>>>> system hasn't been used for a while so I’m inclined to think that > >>>>>> > >>>>>> immersion > >>>>>>>> > >>>>>>>> oil was left on the objective for more than a year. > >>>>>>>> > >>>>>>>> Have you ever seen anything like that and do you have any > suggestion > >>>>>> > >>>>>> as to > >>>>>>>> > >>>>>>>> how I should start the cleaning? I’m afraid to use my standard > >>>>>>>> cleaning > >>>>>>>> protocol as the particles inside the oil could scratch the lens. > >>>>>>>> > >>>>>>>> Thanks for your help. > >>>>>>>> *Gabriel Lapointe, M.Sc.* > >>>>>>>> Lab Manager / Microscopy Specialist > >>>>>>>> Concordia University, Biology Department > >>>>>>>> 7141 Sherbrooke St. West SP 534 > >>>>>>>> Montréal QC H4B 1R6 Canada > >>>>>>>> Lab : (514) 848-2424 x5988 > >>>>>>>> Office : (514) 848-2424 x3008 > >>>>>>>> Fax : (514) 848-2881 > >>>>>>>> Cell : (514) 278-0247 > >>>>>>>> [hidden email] > >>>>>>>> cmac.concordia.ca > >>>>>>>> http://gabriellapointe.ca > >>>>>> > >>>>>> > >>>>> > >>>>> > >>>>> > >>>>> -- > >>>>> > >>>>> > >>>>> Joel B. Sheffield, Ph.D > >>>>> Department of Biology > >>>>> Temple University > >>>>> Philadelphia, PA 19122 > >>>>> Voice: 215 204 8839 > >>>>> e-mail: [hidden email] > >>>>> URL: http://astro.temple.edu/~jbs > >>>>> > >>>> > >>>> > >>>> > >>>> -- > >>>> > >>>> > >>>> Joel B. Sheffield, Ph.D > >>>> Department of Biology > >>>> Temple University > >>>> Philadelphia, PA 19122 > >>>> Voice: 215 204 8839 > >>>> e-mail: [hidden email] > >>>> URL: http://astro.temple.edu/~jbs > >>>> > >>> > >>> > >>> > **************************************************************************** > >>> * Tina (Weatherby) Carvalho * [hidden email] > >>> * > >>> * Biological Electron Microscope Facility * (808) 956-6251 > >>> * > >>> * University of Hawaii at Manoa * > >>> http://www.pbrc.hawaii.edu/bemf* > >>> > >>> > **************************************************************************** > >> > >> > >> -- > >> Matthew Nicholas > >> Medical Scientist Training Program Student > >> Laboratory of Arne Gennerich > >> Department of Anatomy and Structural Biology > >> Albert Einstein College of Medicine > >> Forchheimer Building, Room 628 > >> 1300 Morris Park Avenue > >> Bronx, New York 10461 > >> 718.430.3446 > >> [hidden email] > |
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George McNamara |
I suggest warm oil ... Re: Advice on lens cleaning
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In reply to this post by John Oreopoulos
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** When I used leica oil (circa 2000-2005) before moving to Miami and exclusively using Zeiss oil (1.518 "F"), I used to dissolve the (transparent) crystals in the bottom of the leica oil bottle by microwaving. Probably a bad idea to cook your objective lens on high! You (Gabriel) might try warm (37 C, if that does not work, then 47 C, etc) oil, over some time period (24 hours) with the lens suspended and pointing down into the oil (and just the tip in the oil), to see if that dissolves the crystal. This assumes that the crystal is from the oil, rather than some component of a previous experiment. A Leica rep introduced me to an interesting solvent for cleaning organic stuff from objective lenses: methyl ethyl ketone (MEK). Dissolves lots of organic stuff (i.e. oil). Does tend to also remove the anti-reflection coating, which may be a worthwhile tradeoff. Best wishes, George On 4/29/2013 1:43 PM, John Oreopoulos wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Gabriel, > > I should stress that my toluene soaking suggestion has never been tried here in house and was only suggested as a last ditch effort to save a lens in a dire situation like the one you described. The other suggestions made already seem much "milder" and should probably be tried before the method I mentioned. Try at your own discretion!... And do let us know how it all goes afterwards. > > Cheers, > > John Oreopoulos > > > On 2013-04-27, at 7:21 AM, phil laissue wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> The tiniest bit of (warm) distilled water in a small beaker, just >> enough to touch the front lens, may do the trick. And Zeiss also had a >> crystallising immersion oil several years ago, without green crystals >> though. >> _____________________________________ >> Philippe Laissue, PhD, Bioimaging Manager >> School of Biological Sciences, Room 4.17 >> University of Essex, Colchester CO4 3SQ, UK >> (0044) 01206 872246 / (0044) 07842 676 456 >> [hidden email] >> privatewww.essex.ac.uk/~plaissue >> >> >> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas >> <[hidden email]> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> I've never dealt with this problem before, so this may be off the mark, but >>> what about using more of the oil itself to dissolve the crystals? >>> >>> Good luck! >>> Matt >>> >>> On 4/26/13 10:47 PM, Tina Carvalho wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> True, that. I had green crystals form in Leica oil as well! I can't >>>> remember for sure, but I think we heated it to dissolve them, or at least >>>> mobilize the oil enough that could be gently wiped off before trying any >>>> cleaner. >>>> >>>> Good luck and aloha, >>>> Tina >>>> >>>> >>>>> I should add that the oil with the crystals was from Leica. >>>>> Joel >>>>> >>>>> >>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD<[hidden email]> >>>>> wrote: >>>>> >>>>> >>>>>> Wow! Toluene? We have had the experience of immersion oil crystalizing >>>>>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >>>>>> warm bath would be helpful in this case, or just warming the lens to 38 >>>>>> or >>>>>> so.. >>>>>> >>>>>> Joel >>>>>> >>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos< >>>>>> [hidden email]> wrote: >>>>>> >>>>>> >>>>>>> ***** >>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>> ***** >>>>>>> >>>>>>> >>>>>>>> Hey Gabriel, >>>>>>>> >>>>>>>> I consulted with one of the optics experts over here and he recommends >>>>>>>> >>>>>>> the following: >>>>>>> >>>>>>>> >>>>>>>> Fill a beaker with toluene and create a rig above the beaker that will >>>>>>>> >>>>>>> allow you to hold the tip of the objective with the oily crystals >>>>>>> submerged >>>>>>> in the toluene. Leave it there for a week, take it out and then >>>>>>> re-examine >>>>>>> the lens. Hopefully you can use standard cleaning procedures after that >>>>>>> for >>>>>>> any residual muck. Best of luck! >>>>>>> >>>>>>>> >>>>>>>> John Oreopoulos >>>>>>>> Staff Scientist >>>>>>>> Spectral Applied Research >>>>>>>> Richmond Hill, Ontario >>>>>>>> Canada >>>>>>>> www.spectral.ca >>>>>>>> >>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe< >>>>>>>> >>>>>>> [hidden email]> wrote: >>>>>>> >>>>>>>> >>>>>>>> >>>>>>>>> ***** >>>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>>> ***** >>>>>>>>> >>>>>>>>> I have a problem with a dirty objective installed on a straight Leica >>>>>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>>>>> solid >>>>>>>>> dark green particles and the metal edge with green oily crystal. That >>>>>>>>> system hasn't been used for a while so I’m inclined to think that >>>>>>>>> >>>>>>> immersion >>>>>>> >>>>>>>>> oil was left on the objective for more than a year. >>>>>>>>> >>>>>>>>> Have you ever seen anything like that and do you have any suggestion >>>>>>>>> >>>>>>> as to >>>>>>> >>>>>>>>> how I should start the cleaning? I’m afraid to use my standard >>>>>>>>> cleaning >>>>>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>>>>> >>>>>>>>> Thanks for your help. >>>>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>>>> Lab Manager / Microscopy Specialist >>>>>>>>> Concordia University, Biology Department >>>>>>>>> 7141 Sherbrooke St. West SP 534 >>>>>>>>> Montréal QC H4B 1R6 Canada >>>>>>>>> Lab : (514) 848-2424 x5988 >>>>>>>>> Office : (514) 848-2424 x3008 >>>>>>>>> Fax : (514) 848-2881 >>>>>>>>> Cell : (514) 278-0247 >>>>>>>>> [hidden email] >>>>>>>>> cmac.concordia.ca >>>>>>>>> http://gabriellapointe.ca >>>>>>>>> >>>>>>> >>>>>>> >>>>>> >>>>>> >>>>>> -- >>>>>> >>>>>> >>>>>> Joel B. Sheffield, Ph.D >>>>>> Department of Biology >>>>>> Temple University >>>>>> Philadelphia, PA 19122 >>>>>> Voice: 215 204 8839 >>>>>> e-mail: [hidden email] >>>>>> URL: http://astro.temple.edu/~jbs >>>>>> >>>>>> >>>>> >>>>> >>>>> -- >>>>> >>>>> >>>>> Joel B. Sheffield, Ph.D >>>>> Department of Biology >>>>> Temple University >>>>> Philadelphia, PA 19122 >>>>> Voice: 215 204 8839 >>>>> e-mail: [hidden email] >>>>> URL: http://astro.temple.edu/~jbs >>>>> >>>>> >>>> >>>> **************************************************************************** >>>> * Tina (Weatherby) Carvalho * [hidden email] >>>> * >>>> * Biological Electron Microscope Facility * (808) 956-6251 >>>> * >>>> * University of Hawaii at Manoa * >>>> http://www.pbrc.hawaii.edu/bemf* >>>> >>>> **************************************************************************** >>>> >>> >>> -- >>> Matthew Nicholas >>> Medical Scientist Training Program Student >>> Laboratory of Arne Gennerich >>> Department of Anatomy and Structural Biology >>> Albert Einstein College of Medicine >>> Forchheimer Building, Room 628 >>> 1300 Morris Park Avenue >>> Bronx, New York 10461 >>> 718.430.3446 >>> [hidden email] >>> > |
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In reply to this post by Craig Brideau
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I don't know if anyone said this, but the crystals could be an inorganic component? The green colour could be from the salt leaching copper from the brass of the objective. I would try hot (not boiling) water plus detergent first before moving up the scale of aggressive solvents .(Speaking from experience having re-glued an objective lens due to overzealous use of acetone by a colleague -a tricky operation down a stereo microscope). HTH Mark On 29/04/2013, at 11:10 PM, Craig Brideau <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > What John said, but maybe try a Methanol soak before resorting to toluene. > I find it does a decent job of breaking up lens oil in general. I'm not > sure how it will work on your crystals, but if the warm water bath doesn't > work, maybe try a methanol bath? > > Craig > > > On Mon, Apr 29, 2013 at 12:43 PM, John Oreopoulos < > [hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Gabriel, >> >> I should stress that my toluene soaking suggestion has never been tried >> here in house and was only suggested as a last ditch effort to save a lens >> in a dire situation like the one you described. The other suggestions made >> already seem much "milder" and should probably be tried before the method I >> mentioned. Try at your own discretion!... And do let us know how it all >> goes afterwards. >> >> Cheers, >> >> John Oreopoulos >> >> >> On 2013-04-27, at 7:21 AM, phil laissue wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> The tiniest bit of (warm) distilled water in a small beaker, just >>> enough to touch the front lens, may do the trick. And Zeiss also had a >>> crystallising immersion oil several years ago, without green crystals >>> though. >>> _____________________________________ >>> Philippe Laissue, PhD, Bioimaging Manager >>> School of Biological Sciences, Room 4.17 >>> University of Essex, Colchester CO4 3SQ, UK >>> (0044) 01206 872246 / (0044) 07842 676 456 >>> [hidden email] >>> privatewww.essex.ac.uk/~plaissue >>> >>> >>> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas >>> <[hidden email]> wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> I've never dealt with this problem before, so this may be off the mark, >> but >>>> what about using more of the oil itself to dissolve the crystals? >>>> >>>> Good luck! >>>> Matt >>>> >>>> On 4/26/13 10:47 PM, Tina Carvalho wrote: >>>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> True, that. I had green crystals form in Leica oil as well! I can't >>>>> remember for sure, but I think we heated it to dissolve them, or at >> least >>>>> mobilize the oil enough that could be gently wiped off before trying >> any >>>>> cleaner. >>>>> >>>>> Good luck and aloha, >>>>> Tina >>>>> >>>>>> >>>>>> I should add that the oil with the crystals was from Leica. >>>>>> Joel >>>>>> >>>>>> >>>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[hidden email]> >>>>>> wrote: >>>>>> >>>>>>> Wow! Toluene? We have had the experience of immersion oil >> crystalizing >>>>>>> in the vial. Heating it slightly made the crystals dissolve. >> Perhaps a >>>>>>> warm bath would be helpful in this case, or just warming the lens to >> 38 >>>>>>> or >>>>>>> so.. >>>>>>> >>>>>>> Joel >>>>>>> >>>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >>>>>>> [hidden email]> wrote: >>>>>>> >>>>>>>> ***** >>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>> ***** >>>>>>>> >>>>>>>>> Hey Gabriel, >>>>>>>>> >>>>>>>>> I consulted with one of the optics experts over here and he >> recommends >>>>>>>> >>>>>>>> the following: >>>>>>>>> >>>>>>>>> >>>>>>>>> Fill a beaker with toluene and create a rig above the beaker that >> will >>>>>>>> >>>>>>>> allow you to hold the tip of the objective with the oily crystals >>>>>>>> submerged >>>>>>>> in the toluene. Leave it there for a week, take it out and then >>>>>>>> re-examine >>>>>>>> the lens. Hopefully you can use standard cleaning procedures after >> that >>>>>>>> for >>>>>>>> any residual muck. Best of luck! >>>>>>>>> >>>>>>>>> >>>>>>>>> John Oreopoulos >>>>>>>>> Staff Scientist >>>>>>>>> Spectral Applied Research >>>>>>>>> Richmond Hill, Ontario >>>>>>>>> Canada >>>>>>>>> www.spectral.ca >>>>>>>>> >>>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>>>>>>> >>>>>>>> [hidden email]> wrote: >>>>>>>>> >>>>>>>>> >>>>>>>>>> ***** >>>>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>>>> ***** >>>>>>>>>> >>>>>>>>>> I have a problem with a dirty objective installed on a straight >> Leica >>>>>>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>>>>>> solid >>>>>>>>>> dark green particles and the metal edge with green oily crystal. >> That >>>>>>>>>> system hasn't been used for a while so I’m inclined to think that >>>>>>>> >>>>>>>> immersion >>>>>>>>>> >>>>>>>>>> oil was left on the objective for more than a year. >>>>>>>>>> >>>>>>>>>> Have you ever seen anything like that and do you have any >> suggestion >>>>>>>> >>>>>>>> as to >>>>>>>>>> >>>>>>>>>> how I should start the cleaning? I’m afraid to use my standard >>>>>>>>>> cleaning >>>>>>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>>>>>> >>>>>>>>>> Thanks for your help. >>>>>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>>>>> Lab Manager / Microscopy Specialist >>>>>>>>>> Concordia University, Biology Department >>>>>>>>>> 7141 Sherbrooke St. West SP 534 >>>>>>>>>> Montréal QC H4B 1R6 Canada >>>>>>>>>> Lab : (514) 848-2424 x5988 >>>>>>>>>> Office : (514) 848-2424 x3008 >>>>>>>>>> Fax : (514) 848-2881 >>>>>>>>>> Cell : (514) 278-0247 >>>>>>>>>> [hidden email] >>>>>>>>>> cmac.concordia.ca >>>>>>>>>> http://gabriellapointe.ca >>>>>>>> >>>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> -- >>>>>>> >>>>>>> >>>>>>> Joel B. Sheffield, Ph.D >>>>>>> Department of Biology >>>>>>> Temple University >>>>>>> Philadelphia, PA 19122 >>>>>>> Voice: 215 204 8839 >>>>>>> e-mail: [hidden email] >>>>>>> URL: http://astro.temple.edu/~jbs >>>>>>> >>>>>> >>>>>> >>>>>> >>>>>> -- >>>>>> >>>>>> >>>>>> Joel B. Sheffield, Ph.D >>>>>> Department of Biology >>>>>> Temple University >>>>>> Philadelphia, PA 19122 >>>>>> Voice: 215 204 8839 >>>>>> e-mail: [hidden email] >>>>>> URL: http://astro.temple.edu/~jbs >>>>>> >>>>> >>>>> >>>>> >> **************************************************************************** >>>>> * Tina (Weatherby) Carvalho * [hidden email] >>>>> * >>>>> * Biological Electron Microscope Facility * (808) 956-6251 >>>>> * >>>>> * University of Hawaii at Manoa * >>>>> http://www.pbrc.hawaii.edu/bemf* >>>>> >>>>> >> **************************************************************************** >>>> >>>> >>>> -- >>>> Matthew Nicholas >>>> Medical Scientist Training Program Student >>>> Laboratory of Arne Gennerich >>>> Department of Anatomy and Structural Biology >>>> Albert Einstein College of Medicine >>>> Forchheimer Building, Room 628 >>>> 1300 Morris Park Avenue >>>> Bronx, New York 10461 >>>> 718.430.3446 >>>> [hidden email] >> Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology & Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
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In reply to this post by George McNamara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** So far as I am aware Leica and Zeiss both use the same oils from the same supplier. Guy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara Sent: Tuesday, 30 April 2013 2:42 PM To: [hidden email] Subject: I suggest warm oil ... Re: Advice on lens cleaning ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** When I used leica oil (circa 2000-2005) before moving to Miami and exclusively using Zeiss oil (1.518 "F"), I used to dissolve the (transparent) crystals in the bottom of the leica oil bottle by microwaving. Probably a bad idea to cook your objective lens on high! You (Gabriel) might try warm (37 C, if that does not work, then 47 C, etc) oil, over some time period (24 hours) with the lens suspended and pointing down into the oil (and just the tip in the oil), to see if that dissolves the crystal. This assumes that the crystal is from the oil, rather than some component of a previous experiment. A Leica rep introduced me to an interesting solvent for cleaning organic stuff from objective lenses: methyl ethyl ketone (MEK). Dissolves lots of organic stuff (i.e. oil). Does tend to also remove the anti-reflection coating, which may be a worthwhile tradeoff. Best wishes, George On 4/29/2013 1:43 PM, John Oreopoulos wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Gabriel, > > I should stress that my toluene soaking suggestion has never been tried here in house and was only suggested as a last ditch effort to save a lens in a dire situation like the one you described. The other suggestions made already seem much "milder" and should probably be tried before the method I mentioned. Try at your own discretion!... And do let us know how it all goes afterwards. > > Cheers, > > John Oreopoulos > > > On 2013-04-27, at 7:21 AM, phil laissue wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> The tiniest bit of (warm) distilled water in a small beaker, just >> enough to touch the front lens, may do the trick. And Zeiss also had a >> crystallising immersion oil several years ago, without green crystals >> though. >> _____________________________________ >> Philippe Laissue, PhD, Bioimaging Manager >> School of Biological Sciences, Room 4.17 >> University of Essex, Colchester CO4 3SQ, UK >> (0044) 01206 872246 / (0044) 07842 676 456 >> [hidden email] >> privatewww.essex.ac.uk/~plaissue >> >> >> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas >> <[hidden email]> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> I've never dealt with this problem before, so this may be off the mark, but >>> what about using more of the oil itself to dissolve the crystals? >>> >>> Good luck! >>> Matt >>> >>> On 4/26/13 10:47 PM, Tina Carvalho wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> True, that. I had green crystals form in Leica oil as well! I can't >>>> remember for sure, but I think we heated it to dissolve them, or at least >>>> mobilize the oil enough that could be gently wiped off before trying any >>>> cleaner. >>>> >>>> Good luck and aloha, >>>> Tina >>>> >>>> >>>>> I should add that the oil with the crystals was from Leica. >>>>> Joel >>>>> >>>>> >>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD<[hidden email]> >>>>> wrote: >>>>> >>>>> >>>>>> Wow! Toluene? We have had the experience of immersion oil crystalizing >>>>>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a >>>>>> warm bath would be helpful in this case, or just warming the lens to 38 >>>>>> or >>>>>> so.. >>>>>> >>>>>> Joel >>>>>> >>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos< >>>>>> [hidden email]> wrote: >>>>>> >>>>>> >>>>>>> ***** >>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>> ***** >>>>>>> >>>>>>> >>>>>>>> Hey Gabriel, >>>>>>>> >>>>>>>> I consulted with one of the optics experts over here and he recommends >>>>>>>> >>>>>>> the following: >>>>>>> >>>>>>>> >>>>>>>> Fill a beaker with toluene and create a rig above the beaker that will >>>>>>>> >>>>>>> allow you to hold the tip of the objective with the oily crystals >>>>>>> submerged >>>>>>> in the toluene. Leave it there for a week, take it out and then >>>>>>> re-examine >>>>>>> the lens. Hopefully you can use standard cleaning procedures after that >>>>>>> for >>>>>>> any residual muck. Best of luck! >>>>>>> >>>>>>>> >>>>>>>> John Oreopoulos >>>>>>>> Staff Scientist >>>>>>>> Spectral Applied Research >>>>>>>> Richmond Hill, Ontario >>>>>>>> Canada >>>>>>>> www.spectral.ca >>>>>>>> >>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe< >>>>>>>> >>>>>>> [hidden email]> wrote: >>>>>>> >>>>>>>> >>>>>>>> >>>>>>>>> ***** >>>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>>> ***** >>>>>>>>> >>>>>>>>> I have a problem with a dirty objective installed on a straight Leica >>>>>>>>> microscope. The lens is covered in what looks like oil mixed with >>>>>>>>> solid >>>>>>>>> dark green particles and the metal edge with green oily crystal. That >>>>>>>>> system hasn't been used for a while so I'm inclined to think that >>>>>>>>> >>>>>>> immersion >>>>>>> >>>>>>>>> oil was left on the objective for more than a year. >>>>>>>>> >>>>>>>>> Have you ever seen anything like that and do you have any suggestion >>>>>>>>> >>>>>>> as to >>>>>>> >>>>>>>>> how I should start the cleaning? I'm afraid to use my standard >>>>>>>>> cleaning >>>>>>>>> protocol as the particles inside the oil could scratch the lens. >>>>>>>>> >>>>>>>>> Thanks for your help. >>>>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>>>> Lab Manager / Microscopy Specialist >>>>>>>>> Concordia University, Biology Department >>>>>>>>> 7141 Sherbrooke St. West SP 534 >>>>>>>>> Montréal QC H4B 1R6 Canada >>>>>>>>> Lab : (514) 848-2424 x5988 >>>>>>>>> Office : (514) 848-2424 x3008 >>>>>>>>> Fax : (514) 848-2881 >>>>>>>>> Cell : (514) 278-0247 >>>>>>>>> [hidden email] >>>>>>>>> cmac.concordia.ca >>>>>>>>> http://gabriellapointe.ca >>>>>>>>> >>>>>>> >>>>>>> >>>>>> >>>>>> >>>>>> -- >>>>>> >>>>>> >>>>>> Joel B. Sheffield, Ph.D >>>>>> Department of Biology >>>>>> Temple University >>>>>> Philadelphia, PA 19122 >>>>>> Voice: 215 204 8839 >>>>>> e-mail: [hidden email] >>>>>> URL: http://astro.temple.edu/~jbs >>>>>> >>>>>> >>>>> >>>>> >>>>> -- >>>>> >>>>> >>>>> Joel B. Sheffield, Ph.D >>>>> Department of Biology >>>>> Temple University >>>>> Philadelphia, PA 19122 >>>>> Voice: 215 204 8839 >>>>> e-mail: [hidden email] >>>>> URL: http://astro.temple.edu/~jbs >>>>> >>>>> >>>> >>>> **************************************************************************** >>>> * Tina (Weatherby) Carvalho * [hidden email] >>>> * >>>> * Biological Electron Microscope Facility * (808) 956-6251 >>>> * >>>> * University of Hawaii at Manoa * >>>> http://www.pbrc.hawaii.edu/bemf* >>>> >>>> **************************************************************************** >>>> >>> >>> -- >>> Matthew Nicholas >>> Medical Scientist Training Program Student >>> Laboratory of Arne Gennerich >>> Department of Anatomy and Structural Biology >>> Albert Einstein College of Medicine >>> Forchheimer Building, Room 628 >>> 1300 Morris Park Avenue >>> Bronx, New York 10461 >>> 718.430.3446 >>> [hidden email] >>> > |
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Martin Wessendorf-2 |
Re: Advice on lens cleaning
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In reply to this post by Mark Cannell-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On 4/30/2013 12:59 AM, Mark Cannell wrote: > I don't know if anyone said this, but the crystals could be an inorganic component? The green colour could be from the salt leaching copper from the brass of the objective. I would try hot (not boiling) water plus detergent first before moving up the scale of aggressive solvents .(Speaking from experience having re-glued an objective lens due to overzealous use of acetone by a colleague -a tricky operation down a stereo microscope). Seconded. If you don't know what the crystals are (e.g., if there's any chance that some dope got buffer or tissue culture medium on the objective), water might be the best solvent. I strongly recommend starting with Sparkle/lens-cleaner/water+detergent or plain water before going to organic solvents. I vividly remember attempting to use acetone to remove a tar from the inside of a Kimex reaction flask in organic chem lab--it just wouldn't come off so I tried scraping with a spatula, at which time the flask broke. The pieces fell into the bottom of the sink...and the water there immediately dissolved the tar. Good luck! Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail: [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This may sound like a commercial reply, since I have in the past worked for both Nikon and Leica microscope dealerships. I am now unemployed and setting up my own consulting company. I highly recommend that you call your friendly local microscope repair person. They have training in safely cleaning lenses and typically carry a range of solvents with them. One thing that has been implied not been said outright in this discussion so far is DO NOT wipe or rub or in anyway attempt to mechanically remove the accumulated gunk and crystals. I will add my vote to staring with water based approaches. Mild heating may help as heat always helps dissolve salts, but not too much heat or you could introduce unpredictable strains in the lens with excessive heating. Keeping in mind that microscopes and optics do fine in cell culture environments, keep the heat low enough that you can handle the lens with bare hands and you should be OK. Patience will also be critical! Chris Tully Microscopy and Image Analysis Expert [hidden email] 240-475-9753 (c) [image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/> On Tue, Apr 30, 2013 at 9:39 AM, Martin Wessendorf <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > ***** > > On 4/30/2013 12:59 AM, Mark Cannell wrote: > > I don't know if anyone said this, but the crystals could be an inorganic >> component? The green colour could be from the salt leaching copper from the >> brass of the objective. I would try hot (not boiling) water plus detergent >> first before moving up the scale of aggressive solvents .(Speaking from >> experience having re-glued an objective lens due to overzealous use of >> acetone by a colleague -a tricky operation down a stereo microscope). >> > > Seconded. If you don't know what the crystals are (e.g., if there's any > chance that some dope got buffer or tissue culture medium on the > objective), water might be the best solvent. I strongly recommend starting > with Sparkle/lens-cleaner/water+**detergent or plain water before going > to organic solvents. I vividly remember attempting to use acetone to > remove a tar from the inside of a Kimex reaction flask in organic chem > lab--it just wouldn't come off so I tried scraping with a spatula, at which > time the flask broke. The pieces fell into the bottom of the sink...and > the water there immediately dissolved the tar. > > Good luck! > > Martin Wessendorf > -- > Martin Wessendorf, Ph.D. office: (612) 626-0145 > Assoc Prof, Dept Neuroscience lab: (612) 624-2991 > University of Minnesota Preferred FAX: (612) 624-8118 > 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 > Minneapolis, MN 55455 e-mail: [hidden email] > |
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In reply to this post by Mark Cannell-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** What Mark said happened to a Leica objective on our inverted microscope. That objective was stuck on the turret. I saw some light green stuff deposited around the circumference of the objective rear thread. After calling Leica rep, I clean the green stuff with dipping DI water to Q-tips to dissolve the deposit carefully. The light green deposit was most likely copper chlorite, as one group frequently imaged live cell in Tyrode's solution, which contains several chlorites. One user could dropped the solution by accident. I would try warm DI water first, as some suggested already. Best regards, Jingsong Wang, PhD Imaging Core Facilty Child & Family Research Institute Room A4-110, 950 W. 28th. Ave. Vancouver BC V5Z 4H4 Canada -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark Cannell Sent: April-29-13 11:00 PM To: [hidden email] Subject: Re: Advice on lens cleaning ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I don't know if anyone said this, but the crystals could be an inorganic component? The green colour could be from the salt leaching copper from the brass of the objective. I would try hot (not boiling) water plus detergent first before moving up the scale of aggressive solvents .(Speaking from experience having re-glued an objective lens due to overzealous use of acetone by a colleague -a tricky operation down a stereo microscope). HTH Mark On 29/04/2013, at 11:10 PM, Craig Brideau <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > What John said, but maybe try a Methanol soak before resorting to toluene. > I find it does a decent job of breaking up lens oil in general. I'm > not sure how it will work on your crystals, but if the warm water bath > doesn't work, maybe try a methanol bath? > > Craig > > > On Mon, Apr 29, 2013 at 12:43 PM, John Oreopoulos < > [hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Gabriel, >> >> I should stress that my toluene soaking suggestion has never been >> tried here in house and was only suggested as a last ditch effort to >> save a lens in a dire situation like the one you described. The other >> suggestions made already seem much "milder" and should probably be >> tried before the method I mentioned. Try at your own discretion!... >> And do let us know how it all goes afterwards. >> >> Cheers, >> >> John Oreopoulos >> >> >> On 2013-04-27, at 7:21 AM, phil laissue wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> The tiniest bit of (warm) distilled water in a small beaker, just >>> enough to touch the front lens, may do the trick. And Zeiss also had >>> a crystallising immersion oil several years ago, without green >>> crystals though. >>> _____________________________________ >>> Philippe Laissue, PhD, Bioimaging Manager School of Biological >>> Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK >>> (0044) 01206 872246 / (0044) 07842 676 456 [hidden email] >>> privatewww.essex.ac.uk/~plaissue >>> >>> >>> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas >>> <[hidden email]> wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> ***** >>>> >>>> I've never dealt with this problem before, so this may be off the >>>> mark, >> but >>>> what about using more of the oil itself to dissolve the crystals? >>>> >>>> Good luck! >>>> Matt >>>> >>>> On 4/26/13 10:47 PM, Tina Carvalho wrote: >>>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> ***** >>>>> >>>>> True, that. I had green crystals form in Leica oil as well! I >>>>> can't remember for sure, but I think we heated it to dissolve >>>>> them, or at >> least >>>>> mobilize the oil enough that could be gently wiped off before >>>>> trying >> any >>>>> cleaner. >>>>> >>>>> Good luck and aloha, >>>>> Tina >>>>> >>>>>> >>>>>> I should add that the oil with the crystals was from Leica. >>>>>> Joel >>>>>> >>>>>> >>>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD >>>>>> <[hidden email]> >>>>>> wrote: >>>>>> >>>>>>> Wow! Toluene? We have had the experience of immersion oil >> crystalizing >>>>>>> in the vial. Heating it slightly made the crystals dissolve. >> Perhaps a >>>>>>> warm bath would be helpful in this case, or just warming the >>>>>>> lens to >> 38 >>>>>>> or >>>>>>> so.. >>>>>>> >>>>>>> Joel >>>>>>> >>>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos < >>>>>>> [hidden email]> wrote: >>>>>>> >>>>>>>> ***** >>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>> ***** >>>>>>>> >>>>>>>>> Hey Gabriel, >>>>>>>>> >>>>>>>>> I consulted with one of the optics experts over here and he >> recommends >>>>>>>> >>>>>>>> the following: >>>>>>>>> >>>>>>>>> >>>>>>>>> Fill a beaker with toluene and create a rig above the beaker >>>>>>>>> that >> will >>>>>>>> >>>>>>>> allow you to hold the tip of the objective with the oily >>>>>>>> crystals submerged in the toluene. Leave it there for a week, >>>>>>>> take it out and then re-examine the lens. Hopefully you can use >>>>>>>> standard cleaning procedures after >> that >>>>>>>> for >>>>>>>> any residual muck. Best of luck! >>>>>>>>> >>>>>>>>> >>>>>>>>> John Oreopoulos >>>>>>>>> Staff Scientist >>>>>>>>> Spectral Applied Research >>>>>>>>> Richmond Hill, Ontario >>>>>>>>> Canada >>>>>>>>> www.spectral.ca >>>>>>>>> >>>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe < >>>>>>>> >>>>>>>> [hidden email]> wrote: >>>>>>>>> >>>>>>>>> >>>>>>>>>> ***** >>>>>>>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>>>>>>> ***** >>>>>>>>>> >>>>>>>>>> I have a problem with a dirty objective installed on a >>>>>>>>>> straight >> Leica >>>>>>>>>> microscope. The lens is covered in what looks like oil mixed >>>>>>>>>> with solid dark green particles and the metal edge with green >>>>>>>>>> oily crystal. >> That >>>>>>>>>> system hasn't been used for a while so I'm inclined to think >>>>>>>>>> that >>>>>>>> >>>>>>>> immersion >>>>>>>>>> >>>>>>>>>> oil was left on the objective for more than a year. >>>>>>>>>> >>>>>>>>>> Have you ever seen anything like that and do you have any >> suggestion >>>>>>>> >>>>>>>> as to >>>>>>>>>> >>>>>>>>>> how I should start the cleaning? I'm afraid to use my >>>>>>>>>> standard cleaning protocol as the particles inside the oil >>>>>>>>>> could scratch the lens. >>>>>>>>>> >>>>>>>>>> Thanks for your help. >>>>>>>>>> *Gabriel Lapointe, M.Sc.* >>>>>>>>>> Lab Manager / Microscopy Specialist Concordia University, >>>>>>>>>> Biology Department >>>>>>>>>> 7141 Sherbrooke St. West SP 534 Montréal QC H4B 1R6 Canada >>>>>>>>>> Lab : (514) 848-2424 x5988 Office : (514) 848-2424 x3008 Fax >>>>>>>>>> : (514) 848-2881 Cell : (514) 278-0247 >>>>>>>>>> [hidden email] cmac.concordia.ca >>>>>>>>>> http://gabriellapointe.ca >>>>>>>> >>>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> -- >>>>>>> >>>>>>> >>>>>>> Joel B. Sheffield, Ph.D >>>>>>> Department of Biology >>>>>>> Temple University >>>>>>> Philadelphia, PA 19122 >>>>>>> Voice: 215 204 8839 >>>>>>> e-mail: [hidden email] >>>>>>> URL: http://astro.temple.edu/~jbs >>>>>>> >>>>>> >>>>>> >>>>>> >>>>>> -- >>>>>> >>>>>> >>>>>> Joel B. Sheffield, Ph.D >>>>>> Department of Biology >>>>>> Temple University >>>>>> Philadelphia, PA 19122 >>>>>> Voice: 215 204 8839 >>>>>> e-mail: [hidden email] >>>>>> URL: http://astro.temple.edu/~jbs >>>>>> >>>>> >>>>> >>>>> >> ********************************************************************* >> ******* >>>>> * Tina (Weatherby) Carvalho * [hidden email] >>>>> * >>>>> * Biological Electron Microscope Facility * (808) 956-6251 >>>>> * >>>>> * University of Hawaii at Manoa * >>>>> http://www.pbrc.hawaii.edu/bemf* >>>>> >>>>> >> ********************************************************************* >> ******* >>>> >>>> >>>> -- >>>> Matthew Nicholas >>>> Medical Scientist Training Program Student Laboratory of Arne >>>> Gennerich Department of Anatomy and Structural Biology Albert >>>> Einstein College of Medicine Forchheimer Building, Room 628 >>>> 1300 Morris Park Avenue >>>> Bronx, New York 10461 >>>> 718.430.3446 >>>> [hidden email] >> Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology & Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
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Craig Brideau |
Re: I suggest warm oil ... Re: Advice on lens cleaning
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In reply to this post by Guy Cox-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Boiling oil huh? I'll be sure not to lay siege to your lab, you sound prepared. @;-) It's amazing what people manage to get stuck to/on/in the objective lenses. We've managed to stain the nose of one of our dipping lenses with Nile Red. Vinegar is apparently the best way to get that out. Other than that I've mainly had salt crystals and still-gooey lens-oil residue that I was able to take off with either DI water (rince with a tissue underneath to catch the drips) or methanol. Methanol seems to be a good compromise between water and something harsher. Does anyone else have any success with it? Craig On Tue, Apr 30, 2013 at 7:12 AM, Guy Cox <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > So far as I am aware Leica and Zeiss both use the same oils from the same > supplier. > > Guy > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of George McNamara > Sent: Tuesday, 30 April 2013 2:42 PM > To: [hidden email] > Subject: I suggest warm oil ... Re: Advice on lens cleaning > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > When I used leica oil (circa 2000-2005) before moving to Miami and > exclusively using Zeiss oil (1.518 "F"), I used to dissolve the > (transparent) crystals in the bottom of the leica oil bottle by > microwaving. Probably a bad idea to cook your objective lens on high! > You (Gabriel) might try warm (37 C, if that does not work, then 47 C, > etc) oil, over some time period (24 hours) with the lens suspended and > pointing down into the oil (and just the tip in the oil), to see if that > dissolves the crystal. This assumes that the crystal is from the oil, > rather than some component of a previous experiment. > > A Leica rep introduced me to an interesting solvent for cleaning organic > stuff from objective lenses: methyl ethyl ketone (MEK). Dissolves lots > of organic stuff (i.e. oil). Does tend to also remove the > anti-reflection coating, which may be a worthwhile tradeoff. > > Best wishes, > > George > > > On 4/29/2013 1:43 PM, John Oreopoulos wrote: > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Gabriel, > > > > I should stress that my toluene soaking suggestion has never been tried > here in house and was only suggested as a last ditch effort to save a lens > in a dire situation like the one you described. The other suggestions made > already seem much "milder" and should probably be tried before the method I > mentioned. Try at your own discretion!... And do let us know how it all > goes afterwards. > > > > Cheers, > > > > John Oreopoulos > > > > > > On 2013-04-27, at 7:21 AM, phil laissue wrote: > > > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> ***** > >> > >> The tiniest bit of (warm) distilled water in a small beaker, just > >> enough to touch the front lens, may do the trick. And Zeiss also had a > >> crystallising immersion oil several years ago, without green crystals > >> though. > >> _____________________________________ > >> Philippe Laissue, PhD, Bioimaging Manager > >> School of Biological Sciences, Room 4.17 > >> University of Essex, Colchester CO4 3SQ, UK > >> (0044) 01206 872246 / (0044) 07842 676 456 > >> [hidden email] > >> privatewww.essex.ac.uk/~plaissue > >> > >> > >> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas > >> <[hidden email]> wrote: > >> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> ***** > >>> > >>> I've never dealt with this problem before, so this may be off the > mark, but > >>> what about using more of the oil itself to dissolve the crystals? > >>> > >>> Good luck! > >>> Matt > >>> > >>> On 4/26/13 10:47 PM, Tina Carvalho wrote: > >>> > >>>> ***** > >>>> To join, leave or search the confocal microscopy listserv, go to: > >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>> ***** > >>>> > >>>> True, that. I had green crystals form in Leica oil as well! I can't > >>>> remember for sure, but I think we heated it to dissolve them, or at > least > >>>> mobilize the oil enough that could be gently wiped off before trying > any > >>>> cleaner. > >>>> > >>>> Good luck and aloha, > >>>> Tina > >>>> > >>>> > >>>>> I should add that the oil with the crystals was from Leica. > >>>>> Joel > >>>>> > >>>>> > >>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD<[hidden email]> > >>>>> wrote: > >>>>> > >>>>> > >>>>>> Wow! Toluene? We have had the experience of immersion oil > crystalizing > >>>>>> in the vial. Heating it slightly made the crystals dissolve. > Perhaps a > >>>>>> warm bath would be helpful in this case, or just warming the lens > to 38 > >>>>>> or > >>>>>> so.. > >>>>>> > >>>>>> Joel > >>>>>> > >>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos< > >>>>>> [hidden email]> wrote: > >>>>>> > >>>>>> > >>>>>>> ***** > >>>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>>> ***** > >>>>>>> > >>>>>>> > >>>>>>>> Hey Gabriel, > >>>>>>>> > >>>>>>>> I consulted with one of the optics experts over here and he > recommends > >>>>>>>> > >>>>>>> the following: > >>>>>>> > >>>>>>>> > >>>>>>>> Fill a beaker with toluene and create a rig above the beaker that > will > >>>>>>>> > >>>>>>> allow you to hold the tip of the objective with the oily crystals > >>>>>>> submerged > >>>>>>> in the toluene. Leave it there for a week, take it out and then > >>>>>>> re-examine > >>>>>>> the lens. Hopefully you can use standard cleaning procedures after > that > >>>>>>> for > >>>>>>> any residual muck. Best of luck! > >>>>>>> > >>>>>>>> > >>>>>>>> John Oreopoulos > >>>>>>>> Staff Scientist > >>>>>>>> Spectral Applied Research > >>>>>>>> Richmond Hill, Ontario > >>>>>>>> Canada > >>>>>>>> www.spectral.ca > >>>>>>>> > >>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe< > >>>>>>>> > >>>>>>> [hidden email]> wrote: > >>>>>>> > >>>>>>>> > >>>>>>>> > >>>>>>>>> ***** > >>>>>>>>> To join, leave or search the confocal microscopy listserv, go to: > >>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>>>>>>>> ***** > >>>>>>>>> > >>>>>>>>> I have a problem with a dirty objective installed on a straight > Leica > >>>>>>>>> microscope. The lens is covered in what looks like oil mixed with > >>>>>>>>> solid > >>>>>>>>> dark green particles and the metal edge with green oily crystal. > That > >>>>>>>>> system hasn't been used for a while so I'm inclined to think that > >>>>>>>>> > >>>>>>> immersion > >>>>>>> > >>>>>>>>> oil was left on the objective for more than a year. > >>>>>>>>> > >>>>>>>>> Have you ever seen anything like that and do you have any > suggestion > >>>>>>>>> > >>>>>>> as to > >>>>>>> > >>>>>>>>> how I should start the cleaning? I'm afraid to use my standard > >>>>>>>>> cleaning > >>>>>>>>> protocol as the particles inside the oil could scratch the lens. > >>>>>>>>> > >>>>>>>>> Thanks for your help. > >>>>>>>>> *Gabriel Lapointe, M.Sc.* > >>>>>>>>> Lab Manager / Microscopy Specialist > >>>>>>>>> Concordia University, Biology Department > >>>>>>>>> 7141 Sherbrooke St. West SP 534 > >>>>>>>>> Montréal QC H4B 1R6 Canada > >>>>>>>>> Lab : (514) 848-2424 x5988 > >>>>>>>>> Office : (514) 848-2424 x3008 > >>>>>>>>> Fax : (514) 848-2881 > >>>>>>>>> Cell : (514) 278-0247 > >>>>>>>>> [hidden email] > >>>>>>>>> cmac.concordia.ca > >>>>>>>>> http://gabriellapointe.ca > >>>>>>>>> > >>>>>>> > >>>>>>> > >>>>>> > >>>>>> > >>>>>> -- > >>>>>> > >>>>>> > >>>>>> Joel B. Sheffield, Ph.D > >>>>>> Department of Biology > >>>>>> Temple University > >>>>>> Philadelphia, PA 19122 > >>>>>> Voice: 215 204 8839 > >>>>>> e-mail: [hidden email] > >>>>>> URL: http://astro.temple.edu/~jbs > >>>>>> > >>>>>> > >>>>> > >>>>> > >>>>> -- > >>>>> > >>>>> > >>>>> Joel B. Sheffield, Ph.D > >>>>> Department of Biology > >>>>> Temple University > >>>>> Philadelphia, PA 19122 > >>>>> Voice: 215 204 8839 > >>>>> e-mail: [hidden email] > >>>>> URL: http://astro.temple.edu/~jbs > >>>>> > >>>>> > >>>> > >>>> > **************************************************************************** > >>>> * Tina (Weatherby) Carvalho * [hidden email] > >>>> * > >>>> * Biological Electron Microscope Facility * (808) 956-6251 > >>>> * > >>>> * University of Hawaii at Manoa * > >>>> http://www.pbrc.hawaii.edu/bemf* > >>>> > >>>> > **************************************************************************** > >>>> > >>> > >>> -- > >>> Matthew Nicholas > >>> Medical Scientist Training Program Student > >>> Laboratory of Arne Gennerich > >>> Department of Anatomy and Structural Biology > >>> Albert Einstein College of Medicine > >>> Forchheimer Building, Room 628 > >>> 1300 Morris Park Avenue > >>> Bronx, New York 10461 > >>> 718.430.3446 > >>> [hidden email] > >>> > > > |
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