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The Nikon A1 is also worth considering. We have used an A1 with
spectral detection for about six months, and overall we feel quite
satisfied. Nikon's "Perfect Focus" feature on the Ti inverted base
has proven effective at maintaining cells in focus during long time
lapse intervals, particularly when climate control does not keep the
room temperature perfectly stable. Spectral detection and regular
scanning match the quality of other scopes that we tested (Zeiss 710,
Olympus FV1000) after granting various positives and negatives for
each unit, and the price was competitive. It was also convenient that
we could easily combine TIRF and confocal scanning on a single
inverted scope body.
If spectral analysis of live cells is a goal then I agree with others
that it is important to choose a spectral unit with multiple-PMT
detection. Zeiss and Nikon (and I believe Leica) offer 32-PMT
systems; I am not sure about Olympus.
All the best,
Tim
Timothy Feinstein
University of Pittsburgh Dept. of Pharmacology and Chemical Biology
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