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If anybody pursues the creation of such a documentary, they should hook up with the curators of the National Museum of Health and Medicine, located on the Walter Reed Campus in Silver Spring, MD. They have an amazing historical collection/display of old microscopes. On Mar 12, 2009, at 10:20 AM, Joel Sheffield wrote:
Michael J. Schell, CIV, USU Dept. Pharmacology Uniformed Services University 4301 Jones Bridge Rd. Bethesda, MD 20814 Tel: 301-295-3249 |
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In reply to this post by John Oreopoulos
I am of the opinion that recent advances in microscopy make a far more compelling
story than its historical past.. Badri Roysam Professor, Department of Electrical, Computer and Systems Engineering Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC) Co-Director, Rensselaer Center for Open Source Software Rensselaer Polytechnic Institute 110 8th Street, Troy, New York 12180-3590, USA. Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: 518-276-8715 Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam ----- Original Message ----- From: Michael Schell [mailto:[hidden email]] To: [hidden email] Subject: Re: A microscopy documentary > If anybody pursues the creation of such a documentary, they should > hook up with the curators of the National Museum of Health and > Medicine, located on the Walter Reed Campus in Silver Spring, MD. > They have an amazing historical collection/display of old microscopes. > > > On Mar 12, 2009, at 10:20 AM, Joel Sheffield wrote: > > > Hi All, > > > > I may have missed it, but it seems to me that one should not omit the > > operator of the fsu website, Michael Davidson, from this discussion. > > His site provides both historical and practical information at all > > levels. I would think that he might be very helpful if such a > > project were to get off the ground. > > > > I would also suggest that such a project be coordinated through a > > microscopy organization (MSA), and that sources of funding be > > explored: NSF (through science education)?, Microscope companies? > > etc. > > > > > > Joel > > > > http://micro.magnet.fsu.edu > > > > Date sent: Mon, 9 Mar 2009 17:48:11 -0400 > > Send reply to: Confocal Microscopy List > > <[hidden email]> > > From: "Jeffrey L. Travis" <[hidden email]> > > Subject: Re: A microscopy documentary > > To: [hidden email] > > > >> The closest thing to a microscopy documentary I can recall is the > >> Saturday Night Live segment of "Leonard Pinth-Garnell's Bad Musicals" > >> which featured a musical based on the life of Leewenhoek. This > >> sketch > >> originally aired on December 10, 1977. A synopsis can be found here: > >> http://snlarc.jt.org/detail.php?i=1977121010 > >> > >> The musical, as I recall, was proclaimed by Pinth-Garnell to be > >> "simply > >> terrible." > > > > > > > > Joel B. Sheffield, Ph.D > > Department of Biology > > Temple University > > Philadelphia, PA 19122 > > Voice: 215 204 8839 > > e-mail: [hidden email] > > URL: http://astro.temple.edu/~jbs > > Michael J. Schell, CIV, USU > Dept. Pharmacology > Uniformed Services University > 4301 Jones Bridge Rd. > Bethesda, MD 20814 > Tel: 301-295-3249 > [hidden email] > http://www.usuhs.mil/pha/mschell.html > > |
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I am of the opinion a historical perspective is a valuable and often essential component of a good story, especially in the context of a documentary. What could be better than first using the history of microscopes to teach about Abbe's Law--and then telling the "recent advances" part about how the Law has been smashed? On Mar 12, 2009, at 10:38 AM, Badri Roysam wrote:
Michael J. Schell, CIV, USU Dept. Pharmacology Uniformed Services University 4301 Jones Bridge Rd. Bethesda, MD 20814 Tel: 301-295-3249 |
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In reply to this post by John Oreopoulos
I agree with the idea of providing historical perspective - but not go as far as to feature hobbies of inventors past... cheers,
Badri Roysam Professor, Department of Electrical, Computer and Systems Engineering Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC) Co-Director, Rensselaer Center for Open Source Software Rensselaer Polytechnic Institute 110 8th Street, Troy, New York 12180-3590, USA. Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: 518-276-8715 Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam ----- Original Message ----- From: Michael Schell [mailto:[hidden email]] To: [hidden email] Subject: Re: A microscopy documentary > I am of the opinion a historical perspective is a valuable and often > essential component of a good story, especially in the context of a > documentary. What could be better than first using the history of > microscopes to teach about Abbe's Law--and then telling the "recent > advances" part about how the Law has been smashed? > > > On Mar 12, 2009, at 10:38 AM, Badri Roysam wrote: > > > I am of the opinion that recent advances in microscopy make a far > > more compelling > > story than its historical past.. > > > > > > Badri Roysam > > Professor, Department of Electrical, Computer and Systems Engineering > > Associate Director, NSF Center for Subsurface Sensing & Imaging > > Systems (CenSSIS ERC) > > Co-Director, Rensselaer Center for Open Source Software > > Rensselaer Polytechnic Institute > > 110 8th Street, Troy, New York 12180-3590, USA. > > Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC > > 6308): 518-276-8207, Fax: 518-276-8715 > > Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam > > > > > > > > > > > Michael J. Schell, CIV, USU > Dept. Pharmacology > Uniformed Services University > 4301 Jones Bridge Rd. > Bethesda, MD 20814 > Tel: 301-295-3249 > [hidden email] > http://www.usuhs.mil/pha/mschell.html > > |
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In reply to this post by Michael Schell
I think both history and modern developments are worth including. For sure then some kind of multiple episode series would be needed to cover the entirety of microscopy. I agree with an earlier comment that M. Davidson has done a fantastic job already on the history of microscopy with the Molecular Expressions website. I think even his own personal story of photographing beer crystals with a microscope and turning these into men's ties is a good story worthy of including in such a documentary.
John Oreopoulos On 12-Mar-09, at 12:45 PM, Michael Schell wrote:
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Since we are apparently taking sides, and I suspect there are
some of us that would secretly like to become TV stars, I need to
point out that the early history of microscopy is, in fact, extremely
compelling. Like the telescope, the development of microscopy was
critical to the enhancement of human perception making the invisible
-> visible. J.J. Lister's work (1820s and 30s) that improved the
design of acrhomatic lenses, as well as lens construction that reduced
spherical aberration resulted in the something that approximated the
modern widefield microscope. His scientific contacts with Airy,
Herschel and Dr. Thomas Hodgkin sort of speaks for itself. His
observations of RBCs with Hodgkin led to the identification of
"Hodgkin's disease."
Probably some of you have read "Microbe Hunters" by
Paul Henry De Kruif, which describes the discoveries of Pasteur and
other medical researchers. Can you imagine what the human world would
be like without all the advances in medicine and biology made possible
by microscopy up through the end of the 19th century!
Well that's my 2 cents.
Mario
P.S. I didn't get a "duplicate error" last time I
posted.
I think both history and modern developments are worth including. For sure then some kind of multiple episode series would be needed to cover the entirety of microscopy. I agree with an earlier comment that M. Davidson has done a fantastic job already on the history of microscopy with the Molecular Expressions website. I think even his own personal story of photographing beer crystals with a microscope and turning these into men's ties is a good story worthy of including in such a documentary. John Oreopoulos On 12-Mar-09, at 12:45 PM, Michael Schell wrote:
-- ________________________________________________________________________________
Mario M. Moronne, Ph.D. [hidden email] [hidden email] |
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In reply to this post by Badri Roysam
All good ideas, I'm sure. As part of our Unit's 50th birthday celebrations I gave an historical talk on microscopy in the Macleay Museum and I'd be glad to make the ppt available to anyone who is serious about getting started on such a project. There are all sorts of fascinating strands in the older parts of the history as well as 21st century developments.
But let's be clear about one thing - NOBODY has yet broken Abbe's resolution limit to any substantial extent. No, Stefan Hell hasn't, Mats Gustafsson hasn't ... etc. Rayleigh's criterion (which applies to fluorescence) may lie smashed in the dust, but not Abbe's. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Badri Roysam Sent: Friday, 13 March 2009 3:50 AM To: [hidden email] Subject: Re: A microscopy documentary I agree with the idea of providing historical perspective - but not go as far as to feature hobbies of inventors past... cheers, Badri Roysam Professor, Department of Electrical, Computer and Systems Engineering Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC) Co-Director, Rensselaer Center for Open Source Software Rensselaer Polytechnic Institute 110 8th Street, Troy, New York 12180-3590, USA. Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: 518-276-8715 Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam ----- Original Message ----- From: Michael Schell [mailto:[hidden email]] To: [hidden email] Subject: Re: A microscopy documentary > I am of the opinion a historical perspective is a valuable and often > essential component of a good story, especially in the context of a > documentary. What could be better than first using the history of > microscopes to teach about Abbe's Law--and then telling the "recent > advances" part about how the Law has been smashed? > > > On Mar 12, 2009, at 10:38 AM, Badri Roysam wrote: > > > I am of the opinion that recent advances in microscopy make a far > > more compelling story than its historical past.. > > > > > > Badri Roysam > > Professor, Department of Electrical, Computer and Systems > > Engineering Associate Director, NSF Center for Subsurface Sensing & > > Imaging Systems (CenSSIS ERC) Co-Director, Rensselaer Center for > > Open Source Software Rensselaer Polytechnic Institute 110 8th > > Street, Troy, New York 12180-3590, USA. > > Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC > > 6308): 518-276-8207, Fax: 518-276-8715 > > Email: [hidden email], Web: http://www.ecse.rpi.edu/~roysam > > > > > > > > > > > Michael J. Schell, CIV, USU > Dept. Pharmacology > Uniformed Services University > 4301 Jones Bridge Rd. > Bethesda, MD 20814 > Tel: 301-295-3249 > [hidden email] > http://www.usuhs.mil/pha/mschell.html > > No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.10/1995 - Release Date: 11/03/2009 8:28 AM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.12/1998 - Release Date: 12/03/2009 6:23 PM |
 
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John Oreopoulos |
EMCCD camera image shifting by one pixel column error
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In reply to this post by Ray Gilbert
Dear listserver, yesterday I discovered a very strange camera error on our TIRF microscope setup that makes use of a Photometrics Cascade 512B EMCCD camera. This camera has been generously loaned to us by Olympus Canada (it's their demo camera). Recently I've been doing long time-lapse imaging of cells by TIRF microscopy, typically 200 ms exposures with a 2 sec interval time, capturing 750 frames. During the playback of these movies, I noticed what looked like an occasional horizontal shaking of the image that seemed to occur randomly during the image capture lasting only a single frame and occurring about 1-3 times every minute. Originally I thought this could simply be attributed to building vibrations or a sign that something was wrong with the TMC optical bench filled with air that microscope sits on. Turns out this is not the case.
Yesterday I dismounted the camera from the microscope and optical bench, slapped a camera lens on the end and focused on some random objects and a test target with bright and dark sides sitting on a table. It seems the "shaking" is in fact due to an image shift by 1 pixel column to the right and seems to occur randomly. Right now I'm not sure if the leftmost column is being replaced with a random noise column of pixels or the rightmost column of pixels of the image, but what's even stranger, this problem seems to be software dependent. Recently we started using this camera with MicroManager and it's with that acquisition program I see this problem. When I switch to ImagePro, I don't see this problem occur as often (I've only observed it to happen once in the 5 testing experiments I mentioned whereas it always happens with MicroManager). I also tried altering some of the camera parameters - the problem occurs whether I'm using frame transfer or normal parallel clocking mode. It is not dependent on the exposure or interval time, and again, it seems to me to be occurring at random time points. Has anyone out there ever encountered a problem like this and if so, is there a workaround or fix for it? Thanks in advance for any help and advice. John Oreopoulos, BSc, PhD Candidate University of Toronto Institute For Biomaterials and Biomedical Engineering Centre For Studies in Molecular Imaging Tel: W:416-946-5022 |
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John Oreopoulos |
Re: EMCCD camera image shifting by one pixel column error
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Dear confocal listserver, I'd just like to follow up on the problem I reported a couple weeks ago. It seems that the issue was indeed hardware related and went away when we replaced the frame grabber computer (PCI) card for the camera with a newer version that we had in the lab. I'm still not exactly sure about the origin of the problem (the electronics and/or communication mechanisms) and why simply replacing the card made it go away, but I thought I'd let everyone know in case they ever run into the same scenario with their instruments. I'd like to personally thank Nico Stuurman from the MicroManager team, Jerry Jaramillo and Mic Chaudoir from Photometrics, and George Sakellaropoulos from Olympus Canada for their rapid assistance and troubleshooting advice. John Oreopoulos On 17-Mar-09, at 9:00 AM, John Oreopoulos wrote: Dear listserver, yesterday I discovered a very strange camera error on our TIRF microscope setup that makes use of a Photometrics Cascade 512B EMCCD camera. This camera has been generously loaned to us by Olympus Canada (it's their demo camera). Recently I've been doing long time-lapse imaging of cells by TIRF microscopy, typically 200 ms exposures with a 2 sec interval time, capturing 750 frames. During the playback of these movies, I noticed what looked like an occasional horizontal shaking of the image that seemed to occur randomly during the image capture lasting only a single frame and occurring about 1-3 times every minute. Originally I thought this could simply be attributed to building vibrations or a sign that something was wrong with the TMC optical bench filled with air that microscope sits on. Turns out this is not the case. |
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