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Hi Shiv,
2 more instruments, both about 15 months old (purchased with 4 year
service contracts). We had not been using the resonant scanner in either
until recently.
SP5 inverted: Tried for the first time 2 weeks ago. Something was
occluding most of the field of view, but we were able to see nice Fluo-3
calcium waves in cardiomyocytes, in one edge of the field. The Leica
service engineer visited Thursday, said the scan head needs to be
replaced, which should happen in less than 2 weeks.
MP/SP5 upright: A colleague who does a lot of Z-series imaging (see
Speier et al 2008 Nature Methods and Nature Medicine for details),
complained last week that since switching from the standard to the
resonant scanner mode, he no longer has time during Z-series for breaks.
He had routinely been using 512x512 pixel mode (10x dipping lens), so no
change of pixel resolution between standard and resonant scanner modes.
He did add (2 frame?) averaging to his acquisition procedure. The other
people using this transplant model are also now switching to the resonant
scanner. I believe the time savings by using resonant scanner mode is
between 3x and 5x.
George
At 04:48 PM 9/5/2008, you wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Listers,
Thank you for many of you responded to my query regarding the TCS SP5
performance and service. Here is the consolidated responses so far as I
have promised earlier.
Comment #1: Both resonant and conventional modes of our tandem scanner
work beautifully. In a moderate usage situation, the microscope has never
broken down in the past 6 months. Field service is reasonably rapid, and
helpful phone service is readily available in regular business hours. I
havent had a chance to compare side-by-side with LSM, but over the many
years as a Leica confocal customer I am overall very satisfied with them
and in particular with SP5.
Comment #2: We considered an SP5 with a resonant scanner as an option
for fast confocal imaging but the Leica engineers couldn't get the
resonant scanner to work on the SP5 (on the installed system we tried).
From that experience I got the sense that the resonant scanner is not
very reliable. They don't use it very much, they stick to the
'regular' scanner. Sensitivity was the same as the Zeiss 510 with
live plant samples expressing FPs. However, this is not out of rigorous
comparison.
Comment #3: Having SP5 system with visible lasers. Most people in the
facility uses regular scanner and the resonant scanner is rarely used so
could not comment on the performance. Satisfied with the performance
overall.
The notes above are modified from the original responses to remove the
institutional and personal information. It is for information purpose
only and not a scientific survey. Please do not use this information
solely to decide on the performance and service of any given systems. I
have no commercial interest and none of the participants in neither of
the companies. It is also shared personally with the Leica,
Sales-Service Headquarters in the USA.
Shiv
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Date: Wed, 27 Aug 2008
12:10:02 -0500
Reply-To: Confocal Microscopy List
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From: Mayandi Sivaguru
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Subject: Re: Leica TCS SP5 Performance/Problems/Service
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Listers,
Absolutely, I will share the offline responses (after consolidation) I
receive on this thread but the identities of the person providing the
information and institution details will be suppressed.
Thanks again
Shiv
At 11:44 AM 8/27/2008, you wrote:
Search the CONFOCAL archive
at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Shiv,
Likewise, I would be most appreciative if you could share any
off-line
responses with me. The Leica TCS SP5 (X) is an instrument we
are
considering for purchase in the near future.
Many thanks,
Laurie
Laurie Stojanovic
Research Associate
Baxter Healthcare Corporation
Microscopy and Particle Sciences
Route 120 & Wilson Road, RL T-12
Round Lake, IL 60073
Phone: 1-(847)-270-4422
FAX: 1-(847)-270-6360
Email:
[hidden email]
"Armstrong,
Brian"
<[hidden email]
To
RG>
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Sent by:
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Microscopy
List
<CONFOCAL@LISTSER
Subject
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Re: Leica TCS
SP5
Performance/Problems/Service
08/27/2008
11:10
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Please respond
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Search the CONFOCAL archive at
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Dear Shiv, if you receive responses off-line can you please share them
with
me and/or the list? I would be most interested in this information.
Cheers,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
1450 E Duarte Rd
Duarte, CA 91010
626-359-8111 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx
From: Confocal Microscopy List [
[hidden email]] On
Behalf Of Mayandi Sivaguru
Sent: Wednesday, August 27, 2008 8:15 AM
To: [hidden email]
Subject: Leica TCS SP5 Performance/Problems/Service
Dear listers, I would greatly appreciate if you could send us a feedback
on
TCS SP5 performance if you have one in your lab/core facility. I would
like
to know the performance of tandem-ressonant scanner, how often you end
up
with system down situations, how soon the problems are solved by Leica
if
you happen to have the service contract, have you ever put a sample under
a
LSM and TCS SP5 and compared the sensitivities of both systems and
finally
overall how satisfied you are on the performance of the TCS SP5.
By the way does anybody in the list have the new Supercontinuum white
laser
installed in the SP5 (X).
You can contact me off the list if you wish to share the information
directly to me.
Thanking you so much in advance for your kind reply.
Shiv
Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA
Office: 217.333.1214
Fax: 217.244.2496
[hidden email]
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Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA
Office: 217.333.1214
Fax: 217.244.2496
[hidden email]
http://core.igb.uiuc.edu
Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA
Office: 217.333.1214
Fax: 217.244.2496
[hidden email]
http://core.igb.uiuc.edu
George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
[hidden email]
[hidden email]
305-243-8436 office
http://home.earthlink.net/~pubspectra/
http://home.earthlink.net/~geomcnamara/
http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc
(Analytical Imaging Core Facility)
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