GE DeltaVision OMX DLM

[Neil Anthony]

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Hi all,
I was just wondering if anybody has used the single molecule localization add on, DLM, on the GE DeltaVision OMX?  If so I'd love to hear your thoughts on resolution, usage, and anything else that struck you.
Thanks in advance for your time.

Neil









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[Ian Dobbie]

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Hi Neil

Our experience is slightly unique but might be informative.

We have a v3 OMX with the DLM module. In our experience the power density is too low for good STORM imaging. However the newer OMXs have a lens for focusing the power down on a smaller area. I have no experience if this is adequate or not.

The reconstruction software is however pretty ropey and we generally use something like rainSTORM for localisation imaging post acquisition.

Ian

Sent from my iPhone

On 27 Oct 2017, at 18:12, Anthony, Neil <[hidden email]<mailto:[hidden email]>> wrote:

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*****

Hi all,
I was just wondering if anybody has used the single molecule localization add on, DLM, on the GE DeltaVision OMX?  If so I'd love to hear your thoughts on resolution, usage, and anything else that struck you.
Thanks in advance for your time.

Neil









________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

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[Neil Anthony]

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Hi Ian, thanks for the input.
We're looking at the v4 with the focusing optic as you mentioned.

Can I please ask which version of SoftWoRx you're running, and whether or not you feel that the increased laser density would help the software?

Also, do you know about the z-res?  I guess might be very different between v3 and v4 of course, but if it's something you've looked into, I'd appreciate your thoughts.

Thanks
Neil


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ian Dobbie
Sent: Friday, October 27, 2017 2:00 PM
To: [hidden email]
Subject: Re: GE DeltaVision OMX DLM

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Hi Neil

Our experience is slightly unique but might be informative.

We have a v3 OMX with the DLM module. In our experience the power density is too low for good STORM imaging. However the newer OMXs have a lens for focusing the power down on a smaller area. I have no experience if this is adequate or not.

The reconstruction software is however pretty ropey and we generally use something like rainSTORM for localisation imaging post acquisition.

Ian

Sent from my iPhone

On 27 Oct 2017, at 18:12, Anthony, Neil <[hidden email]<mailto:[hidden email]>> wrote:

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,
I was just wondering if anybody has used the single molecule localization add on, DLM, on the GE DeltaVision OMX?  If so I'd love to hear your thoughts on resolution, usage, and anything else that struck you.
Thanks in advance for your time.

Neil









________________________________

This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).

[Oliver Biehlmaier-2]

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Hi Neil,

We have an OMX-V4 including the beam concentration lens for DLM. We do not use DLM very regularly but the few times that we used it it worked ok. Still, the laser power for STORM could be higher…. It really depends on your experiments.

Cheers,
Oliver





Oliver Biehlmaier, PhD | Head of Imaging Core Facility  | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
Phone: [phone] | Email: [hidden email]<mailto:[hidden email]> | www.biozentrum.unibas.ch/imcf<http://www.biozentrum.unibas.ch/imcf> | www.microscopynetwork.unibas.ch<http://www.microscopynetwork.unibas.ch>








On 30 Oct 2017, at 15:12, Anthony, Neil <[hidden email]<mailto:[hidden email]>> wrote:

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*****

Hi Ian, thanks for the input.
We're looking at the v4 with the focusing optic as you mentioned.

Can I please ask which version of SoftWoRx you're running, and whether or not you feel that the increased laser density would help the software?

Also, do you know about the z-res?  I guess might be very different between v3 and v4 of course, but if it's something you've looked into, I'd appreciate your thoughts.

Thanks
Neil


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ian Dobbie
Sent: Friday, October 27, 2017 2:00 PM
To: [hidden email]
Subject: Re: GE DeltaVision OMX DLM

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Neil

Our experience is slightly unique but might be informative.

We have a v3 OMX with the DLM module. In our experience the power density is too low for good STORM imaging. However the newer OMXs have a lens for focusing the power down on a smaller area. I have no experience if this is adequate or not.

The reconstruction software is however pretty ropey and we generally use something like rainSTORM for localisation imaging post acquisition.

Ian

Sent from my iPhone

On 27 Oct 2017, at 18:12, Anthony, Neil <[hidden email]<mailto:[hidden email]>> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,
I was just wondering if anybody has used the single molecule localization add on, DLM, on the GE DeltaVision OMX?  If so I'd love to hear your thoughts on resolution, usage, and anything else that struck you.
Thanks in advance for your time.

Neil









________________________________

This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).

[Ian Dobbie]

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"Anthony, Neil" <[hidden email]> writes:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Ian, thanks for the input.
> We're looking at the v4 with the focusing optic as you mentioned.

This definitely should improve things.

> Can I please ask which version of SoftWoRx you're running, and whether
> or not you feel that the increased laser density would help the
> software?

I think we are now on 6.5, I believe there is a version 7.x as well but
I have not yet tried it.

> Also, do you know about the z-res?  I guess might be very different
> between v3 and v4 of course, but if it's something you've looked into,
> I'd appreciate your thoughts.

I'm sorry but the experiments I have been involved in on this system
have all been "2D", so we are not doing any 3D stuff and ignoring Z
position. We have built an OMX drawer with a 50:50 pellicle and
identical emission filters, so you have 2 images with in the same colour
that have slightly different Z focus due to slight shift in the
camera/lens Z position. In theory this should give us good bi-plane STORM
type imaging but I think it has never really been used in anger.

Ian