In model species such as yeast, GFP-actin does not rescue an actin knockout. In a number of mammalian systems, GFP-actin does not behave identically to actin (although some monomer is incorporated into filaments). Thus, the GFP-actin mouse may have had the GFP-actin driven by a promoter weak enough to not swamp out endogenous actin. I wonder if they had trouble maintaining a mouse line with "just right" amounts of GFP-actin expression. This is only a guess. Wasn't a line of GFP-actin mice generated in Switzerland?
Michael
Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD 20814-3220
tel: (301) 295-3249
[hidden email]
>>> Waldo Schmidt <
[hidden email]> 08/17/09 8:58 PM >>>
Hello all,
For a while I have been contemplating using a GFP-actin mouse as I knew it
existed.
However, as I draw to finding this mouse, I do not succeed.
I have tried to communicate with the researchers who developed the HUGE
GFP actin mouse, but have no response as yet.
Are there any other sources of this mouse?
Kind regards
Locked
