GFP and Venus

[Graham Wright-5]

Hello,
Has anyone tried imaging with GFP and Venus in the same sample with any
success? Any experiences with this, good/bad, that anyone can share would be
appreciated.
Thanks,
Graham

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Graham Wright
Microscopy & Imaging Facility

Temasek Life Sciences Laboratory
1 Research Link
National University of Singapore
Singapore 117604

P:    [phone]
E:     [hidden email]
W:    http://microscopy.tll.org.sg 

[Julio Vazquez]

=

Hi Graham, 

We had relatively good results separating the two proteins with linear unmixing on our Zeiss LSM 510 META confocal. We used 10nm steps for the spectral scans, collected spectra for GFP and Venus, and separated the two proteins either post collection or on the fly. I suppose the same could be achieved with other spectral systems. I must add though that the two proteins were quite bright and had little spatial overlap. Separation may be harder to achieve with lower mixed signals...

Julio.

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Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

==

On Nov 24, 2008, at 8:06 PM, Dr. Graham Wright wrote:

Hello,

Has anyone tried imaging with GFP and Venus in the same sample with any success? Any experiences with this, good/bad, that anyone can share would be appreciated.

Thanks,

Graham

-----

Graham Wright

Microscopy & Imaging Facility

Temasek Life Sciences Laboratory

1 Research Link

National University of Singapore

Singapore 117604

P:    [phone]

E:     [hidden email]

W:    http://microscopy.tll.org.sg