GaAsP detectors for multiphoton

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Phillips, Thomas E. Phillips, Thomas E.
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GaAsP detectors for multiphoton

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Leica has the HyD GaAsP detector for single photon work and may be coming out with a NDD version.  Zeiss apparently already has a GaAsP detector for both single photon work and a GaAsP NDD detector for multiphoton work. My question is whether anyone has looked at using the "conventional" non-NDD GaAsP detectors for use with two photon excitation. Do you get an intermediate result with increased depth of excitation and detection? Thanks, Tom

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[hidden email]<mailto:[hidden email]>

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/
Ramshesh, Venkat K Ramshesh, Venkat K
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Re: GaAsP detectors for multiphoton

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Hi all,

 Just to add to the earlier  questions. Is it true that the GaAsP NDD is only offered on the upright microscope configuration and not on the inverted ones yet?

Best,
Venkat

----
Venkat Ramshesh, PhD
Research Instructor/Bioengineer
Advanced Imaging Core
Center for Cell Death, Injury and Regeneration and Hollings Cancer Center
Drug Discovery Bldg. DD 507
70 President Street, MSC 140
MUSC, Charleston, SC 29425
Ph: 843.876.2363
Imaging Core: 843.876.2359
Fax:843.876.2353
E-mail: [hidden email]
Webpage: www.musc.edu/ccdir



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Phillips, Thomas E.
Sent: Thursday, March 08, 2012 10:51 AM
To: [hidden email]
Subject: GaAsP detectors for multiphoton

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Leica has the HyD GaAsP detector for single photon work and may be coming out with a NDD version.  Zeiss apparently already has a GaAsP detector for both single photon work and a GaAsP NDD detector for multiphoton work. My question is whether anyone has looked at using the "conventional" non-NDD GaAsP detectors for use with two photon excitation. Do you get an intermediate result with increased depth of excitation and detection? Thanks, Tom

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[hidden email]<mailto:[hidden email]>

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/
Armstrong, Brian Armstrong, Brian
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Re: GaAsP detectors for multiphoton

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Hello, you can get NDDs on an inverted configuration for the LSM series scopes.
I do not think that there is a difference in the detectors themselves in an NDD and "non-NDD" set up. The difference being only in the light path in that in the case of NDD the path does not go back into the scanhead and is therefore not "descanned".

Cheers,

Brian Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
1500 East Duarte Road
Duarte, CA 91010
626-256-4673 x62872

Light Microscopy Core Facility

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ramshesh, Venkat K
Sent: Thursday, March 08, 2012 8:13 AM
To: [hidden email]
Subject: Re: GaAsP detectors for multiphoton

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,

 Just to add to the earlier  questions. Is it true that the GaAsP NDD is only offered on the upright microscope configuration and not on the inverted ones yet?

Best,
Venkat

----
Venkat Ramshesh, PhD
Research Instructor/Bioengineer
Advanced Imaging Core
Center for Cell Death, Injury and Regeneration and Hollings Cancer Center
Drug Discovery Bldg. DD 507
70 President Street, MSC 140
MUSC, Charleston, SC 29425
Ph: 843.876.2363
Imaging Core: 843.876.2359
Fax:843.876.2353
E-mail: [hidden email]
Webpage: www.musc.edu/ccdir



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Phillips, Thomas E.
Sent: Thursday, March 08, 2012 10:51 AM
To: [hidden email]
Subject: GaAsP detectors for multiphoton

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Leica has the HyD GaAsP detector for single photon work and may be coming out with a NDD version.  Zeiss apparently already has a GaAsP detector for both single photon work and a GaAsP NDD detector for multiphoton work. My question is whether anyone has looked at using the "conventional" non-NDD GaAsP detectors for use with two photon excitation. Do you get an intermediate result with increased depth of excitation and detection? Thanks, Tom

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[hidden email]<mailto:[hidden email]>

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


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