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Friends
maybe you can be interested in these papers
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MICROSCOPY RESEARCH and TECHNIQUE
Gated-sted microscopy with subnanosecond pulsed fiber laser for reducing photobleaching
The spatial resolution of a stimulated emission depletion (STED) microscope is theoretically unlimited and practically determined by the signal-to-noise ratio. Typically, an increase of the STED beam's power leads to an improvement of the effective resolution. However, this improvement may vanish because an increased STED beam's power is often accompanied by an increased photobleaching, which worsen the effective resolution by reducing the signal strength. A way to lower the photobleaching in pulsed STED (P-STED) implementations is to reduce the peak intensity lengthening the pulses duration (for a given average STED beam's power). This also leads to a reduction of the fluorophores quenching, thus a reduction of the effective resolution, but the time-gated detection was proved to be successful in recovering these reductions. Here we demonstrated that a subnanosecond fiber laser beam (pulse width ∼600 ps) reduces the photobleaching with respect to a traditional stretched hundreds picosecond (∼200 ps) beam provided by a Ti:Sapphire laser, without any effective spatial resolution lost.
link:
http://onlinelibrary.wiley.com/doi/10.1002/jemt.22716/abstract----
SCIENTIFIC REPORTS
Intensity Weighted Subtraction Microscopy Approach for Image Contrast and Resolution Enhancement.
We propose and demonstrate a novel subtraction microscopy algorithm, exploiting fluorescence emission difference or switching laser mode and their derivatives for image enhancement. The key novelty of the proposed approach lies in the weighted subtraction coefficient, adjusted pixel-by-pixel with respect to the intensity distributions of initial images. This method produces significant resolution enhancement and minimizes image distortions. Our theoretical and experimental studies demonstrate that this approach can be applied to any optical microscopy techniques, including label free and non-linear methods, where common super-resolution techniques cannot be used.
link:
http://www.nature.com/articles/srep25816------
Soon other paper from IIT Genoa labs
Ciao
Alby... Grand dad of Irene...since may 20th
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ISTITUTO ITALIANO DI TECNOLOGIA
Alberto Diaspro
Nanophysics
Director
Via Morego, 30 – 16163 Genova
t: +39.010.71781.453/762
[hidden email]
www.iit.it/research/lines/nanoscopy-nic-iit
www.iit.it/people/alberto-diaspro
DO NOT MISS! il Festival della Scienza
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