Getting FRAP settings from .czi file

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Wendy Salmon Wendy Salmon
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Getting FRAP settings from .czi file

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Hello friends,
Does anyone know how to get the FRAP settings out of a Zeiss .czi file
other than opening it on the acquisition system and hitting "Reuse"?
Looking for the settings from the "Bleach" dialog (interrations, speed,
wavelength, laser power, etc).  Thanks!
Wishing you peace and good health,
Wendy

--------------------
Wendy C Salmon, M.A.
Manager
W.M. Keck Facility for Biological Imaging
Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142
e: [hidden email]
w: microscopy.wi.mit.edu
Reece, Jeff (NIH/NIDDK) [E]-2 Reece, Jeff (NIH/NIDDK) [E]-2
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Re: Getting FRAP settings from .czi file

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Hi Wendy,

In Fiji/ImageJ using Bio-Formats Importer (from the Plugins menu or dragging the file onto the main Fiji window should work), check the box that says "Display metadata".  You can also check the box that says "Display ROIs" but I haven't checked how accurate those come in.

Cheers,
Jeff

-----Original Message-----
From: Wendy Salmon <[hidden email]>
Sent: Wednesday, May 27, 2020 3:57 PM
To: [hidden email]
Subject: Getting FRAP settings from .czi file

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello friends,
Does anyone know how to get the FRAP settings out of a Zeiss .czi file other than opening it on the acquisition system and hitting "Reuse"?
Looking for the settings from the "Bleach" dialog (interrations, speed, wavelength, laser power, etc).  Thanks!
Wishing you peace and good health,
Wendy

--------------------
Wendy C Salmon, M.A.
Manager
W.M. Keck Facility for Biological Imaging Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142
e: [hidden email]
w: microscopy.wi.mit.edu