Hands-on Confocal Course April 2009

[O'Toole, P]

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CONFOCAL MICROSCOPY

4-Day Intensive Hands-On Course

Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm

Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing

21 24 April 2009
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Les Borland, Carl Zeiss
Chris Wood, Perkin Elmer
and special guest lecture and practical session with Daniel Zicha (CRUK).

Tutorials and practical sessions will cover both basic and advanced
confocal microscopy. The course will enable participants to realise the
potential behind many developing confocal techniques and realise the
simplicity of applying the techniques to a broad range of sample types
and fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY AIMS
Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on
laser scanning confocal.
Day 3: Advanced Techniques covering spectral unmixing and FRET with
fluorescent proteins.
Day 4: Further advances. More samples to explore confocal limits, and
time for participants own samples.

Thursday evening will include a special guest speaker and course meal.

The course will utilise many different sample types ranging from
cultured monolayers to plant cells. A diverse range of fluorescent
labels will also be used, which will include various fluorescent
proteins (CFP, GFP and YFP) and classic antibody labels. Over the four
days, at least three confocal microscopes (2x Zeiss LSM 510 meta scan
heads on both an invert and upright microscope, PerkinElmer UltraVIEW
LCI or VoX) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the
length of the course.  Evening meals will be provided on both Tuesday
and Thursday evenings.

Places are limited to 8 participants to permit full hands-on practice,
so please book early to avoid disappointment.
For further information, please visit the course website at:
www.york.ac.uk/depts/biol/tf/imaging_course.htm
or contact Margaret Newton 01904 328821, [hidden email]



--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
PO Box 373
YORK
YO10 5YW

Tel : [phone]
Fax : [phone]
email : [hidden email]
www.york.ac.uk/biology/tf

[O'Toole, P]

CONFOCAL MICROSCOPY

4-Day Intensive Hands-On Course

Website: www.york.ac.uk/depts/biol/tf/imaging_course.htm

Basics, Live-cell imaging, FRET, FRAP and Spectral Unmixing

15-18 September 2009
Technology Facility, Dept. Biology
University of York, UK

Instructors will include

Peter O'Toole, University of York
Les Borland, Carl Zeiss
Colin Hornby, Perkin Elmer
and special guest lecture and practical session with Daniel Zicha (CRUK).

Tutorials and practical sessions will cover both basic and advanced
confocal microscopy. The course will enable participants to realise the
potential behind many developing confocal techniques and realise the
simplicity of applying the techniques to a broad range of sample types
and fluorescent labels. Both novice and more experienced users welcome.

Preliminary Programme
DAY AIMS
Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.
Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on
laser scanning confocal.
Day 3: Advanced Techniques covering spectral unmixing and FRET with
fluorescent proteins.
Day 4: Further advances. More samples to explore confocal limits, and
time for participants own samples.

Thursday evening will include a special guest speaker and course meal.

The course will utilise many different sample types ranging from
cultured monolayers to plant cells. A diverse range of fluorescent
labels will also be used, which will include various fluorescent
proteins (CFP, GFP and YFP) and classic antibody labels. Over the four
days, at least three confocal microscopes (2x Zeiss LSM meta scan heads
on both an invert and upright microscope 510, 1 Zeiss LSM710,
PerkinElmer UltraVIEW LCI or VoX) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the
length of the course.  Evening meals will be provided on both Tuesday
and Thursday evenings.

Places are limited to 12 participants to permit full hands-on practice,
so please book early to avoid disappointment.
For further information, please visit the course website at:
www.york.ac.uk/depts/biol/tf/imaging-cytometry/imaging_course.htm
or contact Margaret Newton 01904 328821, [hidden email]


--
Dr Peter O'Toole
Head of Imaging and Cytometry
Technology Facility
Department of Biology (Area 15)
University of York
PO Box 373
YORK
YO10 5YW

Tel : [phone]
Fax : [phone]
email : [hidden email]
www.york.ac.uk/biology/tf