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Jason, here is one protocol I have found online, though it wasn't for C. elegans...
http://homepages.wmich.edu/~rossbach/bios312/LabProcedures/Hanging%20drop%20procedure.htmlJason K.
** no commercial interest**
Jason A. Kilgore
Technical Application Scientist
Molecular Probes Labeling and Detection Technologies
Cells Systems Division
T 1 800 955 6288 then option 4, then option 6, or 541 335 0353 . F 541 335 0238
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Ford,Jason R.
Sent: Tuesday, March 27, 2012 10:02 AM
To:
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Subject: Hanging-drop fixation for live-cell imaging of C. elegans embryos
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Hello,
I was wondering if anyone had a nice protocol for Hanging-drop method for live-cell imaging of C. elegans embryos, or perhaps a general hanging-drop method that I could adapt for nematode embryos.
Cheers,
Jason Ford
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