Hela Cells Flourescence

[Amandeep Walia-2]

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Dear All

Can any one tell me why the Hela cells gives flourescence when the are
grown with DMEM media and when they are seen under 488 and 543 lasers they
give flourscence. what to do ??

Regrads
Amandeep walia

[Graham Wright]

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Dear Amandeep,

Previous discussions have attributed autofluorescence to the riboflavin in
the DMEM and possibly the folic acid and phenol red in that and other
culture media:

http://confocal-microscopy-list.588098.n2.nabble.com/fluorescent-medium-td1691899.html
and
http://www.bio.net/bionet/mm/fluorpro/1999-February/001193.html

Depending on your experimental constraints you can try different media, as
suggested in these threads, with less riboflavin. A filter set that can
suppress it is also suggested.

Good luck,
Graham

--
*Graham Wright, PhD*
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

E:   [hidden email]
W:  http://www.imb.a-star.edu.sg/imu/



On 4 June 2012 14:11, Amandeep Walia <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All
>
> Can any one tell me why the Hela cells gives flourescence when the are
> grown with DMEM media and when they are seen under 488 and 543 lasers they
> give flourscence. what to do ??
>
> Regrads
> Amandeep walia
>