Hello Sir/Madam,
How are you doing?I have heard about Image J just now
fromDr.Rieken.I downloaded it.He told me we could analyze all images at
a time using Macros.I would like to get the data of densitometry of
DAPI,Ki67 and H2AX signal in cells.I am taking images as Tiff images.As
we have started project,i am not getting much time to read the whole
manual.I would be glad to you if you could guide me how to find
suitable Macros for this and analyze signal.Thank you very much for your
time.Have a
wonderful day.
Kumar.
The sample data I was referring
| IDRegion |
Area [µm²] |
H2AX |
KI67 |
DAPI |
| 2.000 |
13.668 |
60.854 |
30.205 |
208.546 |
| 3.000 |
6.729 |
72.202 |
29.174 |
235.648 |
| 6.000 |
13.710 |
50.504 |
63.602 |
248.936 |
| 7.000 |
4.203 |
50.172 |
101.242 |
263.459 |
| 10.000 |
10.933 |
40.305 |
63.602 |
179.623 |
| 12.000 |
10.891 |
61.357 |
27.957 |
218.769 |
| 13.000 |
9.088 |
31.796 |
47.991 |
179.834 |
| 14.000 |
2.820 |
26.294 |
36.807 |
244.818 |
| 15.000 |
82.345 |
35.426 |
45.969 |
177.834 |
| 16.000 |
4.602 |
36.843 |
58.702 |
97.308 |
| 17.000 |
34.077 |
43.223 |
37.260 |
406.203 |
| 18.000 |
18.186 |
103.360 |
45.666 |
307.036 |
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