High NA single-objective light-sheet

[Alfred Millett-Sikking]

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Dear all,

Here's some fun weekend reading for those excited by light-sheet microscopy
and open science:

Follow us here: https://twitter.com/AndrewGYork/status/1138963271594020864

Read the publication here:
https://andrewgyork.github.io/high_na_single_objective_lightsheet/

Apologies for the shameless self promotion, only once every >5 years I
promise.

Thanks,

Alfred Millett-Sikking

[Martin Wessendorf-2]

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Dear Alfred--

Personally, I like this kind of self-promotion.  Interesting instrument.

Thanks!

Martin Wessendorf



On 6/14/2019 2:48 PM, Alfred Millett-Sikking wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Here's some fun weekend reading for those excited by light-sheet microscopy
> and open science:
>
> Follow us here: https://twitter.com/AndrewGYork/status/1138963271594020864
>
> Read the publication here:
> https://andrewgyork.github.io/high_na_single_objective_lightsheet/
>
> Apologies for the shameless self promotion, only once every >5 years I
> promise.
>
> Thanks,
>
> Alfred Millett-Sikking

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]
My preferred pronouns are "he", "him", and "his"

[Steffen Dietzel]

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Dear Alfred,

thanks for sharing.

I am wondering, what is the difference to the SCAPE approach from E.
Hillman's group? (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4317333/)
The beam path geometry appears to be similar.

Sorry if this is not obvious to me.

Best

Steffen

Am 14.06.2019 um 21:48 schrieb Alfred Millett-Sikking:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Here's some fun weekend reading for those excited by light-sheet microscopy
> and open science:
>
> Follow us here: https://twitter.com/AndrewGYork/status/1138963271594020864
>
> Read the publication here:
> https://andrewgyork.github.io/high_na_single_objective_lightsheet/
>
> Apologies for the shameless self promotion, only once every >5 years I
> promise.
>
> Thanks,
>
> Alfred Millett-Sikking
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de

[Alfred Millett-Sikking]

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Steffen,

Yes unlike previous implementations "we sacrifice no appreciable numerical
aperture with no measurable loss in resolution". My Focus on Microscopy
2019 presentation slides
<https://docs.google.com/presentation/d/e/2PACX-1vRIwXpX4IkhVHNha-SlVtEIfNCyUP2Lgmd0w3sIYsaPcLyWO93ec1NDzQRGg6Ws8N22PvYaNCGyIjB9/pub?start=false&loop=false&delayms=60000&slide=id.p>
give a nice overview of how this works. Link also available on website:

https://andrewgyork.github.io/high_na_single_objective_lightsheet/

Thanks for your interest,

Alfred.


On Mon, 17 Jun 2019 at 05:07, Steffen Dietzel <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Alfred,
>
> thanks for sharing.
>
> I am wondering, what is the difference to the SCAPE approach from E.
> Hillman's group? (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4317333/)
> The beam path geometry appears to be similar.
>
> Sorry if this is not obvious to me.
>
> Best
>
> Steffen
>
> Am 14.06.2019 um 21:48 schrieb Alfred Millett-Sikking:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear all,
> >
> > Here's some fun weekend reading for those excited by light-sheet
> microscopy
> > and open science:
> >
> > Follow us here:
> https://twitter.com/AndrewGYork/status/1138963271594020864
> >
> > Read the publication here:
> > https://andrewgyork.github.io/high_na_single_objective_lightsheet/
> >
> > Apologies for the shameless self promotion, only once every >5 years I
> > promise.
> >
> > Thanks,
> >
> > Alfred Millett-Sikking
> >
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Biomedical Center (BMC)
> Head of the Core Facility Bioimaging
>
> Großhaderner Straße 9
> D-82152 Planegg-Martinsried
> Germany
>
> http://www.bioimaging.bmc.med.uni-muenchen.de
>