How to change gas while imaging

[Sandy Simon]

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From: Sandy Simon [mailto:[hidden email]]
Sent: Wednesday, July 30, 2008 10:30 PM
To: '[hidden email]'
Cc: '[hidden email]'
Subject: bubbling media and imaging

Simon,

We have tried bubbling the media - however, if we ever have serum in it, we get frothing.  We can minimize the problem by using an aquarium device.

For rapid changes of pCO2 or pO2, I have bubble the gas through water (heated to 37) to saturate the gas, and then blow this gas right over the imaging chamber.

We have been able to step the pCO2 from 2% to 5% to 10% in < 2 seconds.

 

Two examples where we have used this:

N. Jouvenet, P. D. Bieniasz, and S. M. Simon. Imaging the biogenesis of individual HIV-1 virions in live cells. Nature (London) 454 (7201):236-240, 2008.

S. M. Simon, D. Roy, and M. Schindler. Intracellular pH and the control of multidrug resistance. Proc.Natl.Acad.Sci.U.S.A. 91:1128-1132, 1994.

 

Contact me off-list if you'd like further info.

Regards,

Sandy (the other Simon)

 

Sanford M. Simon

The Rockefeller University

1230 York Avenue

New York, NY 10065

212-327-8130

[hidden email]