Humidity cell requirements for confocal microscopy

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Jan Gorgol Jan Gorgol
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Humidity cell requirements for confocal microscopy

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Dear Listers,

I'm looking for confocal users feedback on their needs regarding humidity cells for Live cell light microscopy applications, namely RH and temperature range they require and the size of the cell that they require and whether it needs to be able to accommodate Petri dish style sample holders such as fluoro dishs (35mm diameter)?Any other comments or feedback helpful on your experience and needs in this area.

Thank you for your help and guidance on this?

Thank you, All the Best

Jan.

Jan Gorgol, Business Development Manager,
Surface Measurement Systems Limited,5 Wharfside,Rosemont Road,Alperton,London HA0 4PE.UK
Tel: + 44 (0)208 795 9400 ,Fax:+ 44 (0)208 795 9401 ,Direct +44 (0)208 795 9408
Email: [hidden email]<mailto:[hidden email]>
George McNamara George McNamara
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Re: Humidity cell requirements for confocal microscopy

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Hi Jan,

Mammalian cells need to be under saline all the time, so relative
humidity there is always 100%. Above the media, yes, needs to be 100% -
this would cause condensation on the lid, unless the lid is warmed a
little (if inverted epi-fluorescence only, the water on the lid does not
matter; evaporation of the media always matters).

Yes, 35 mm glass bottom dishes should be supported.

Unless you are going to be able to offer as good (or better) products
for a lot lower end user price, and as good or better service, than
pecon http://www.pecon.biz/?page_id=48  LIS http://www.lis.ch/ etc,
don't bother.

George


On 12/14/2012 8:21 AM, Jan Gorgol wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Listers,
>
> I'm looking for confocal users feedback on their needs regarding humidity cells for Live cell light microscopy applications, namely RH and temperature range they require and the size of the cell that they require and whether it needs to be able to accommodate Petri dish style sample holders such as fluoro dishs (35mm diameter)?Any other comments or feedback helpful on your experience and needs in this area.
>
> Thank you for your help and guidance on this?
>
> Thank you, All the Best
>
> Jan.
>
> Jan Gorgol, Business Development Manager,
> Surface Measurement Systems Limited,5 Wharfside,Rosemont Road,Alperton,London HA0 4PE.UK
> Tel: + 44 (0)208 795 9400 ,Fax:+ 44 (0)208 795 9401 ,Direct +44 (0)208 795 9408
> Email: [hidden email]<mailto:[hidden email]>
>
>