Humidity in microscope rooms

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Andreas Bruckbauer Andreas Bruckbauer
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Humidity in microscope rooms

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Hi,
what would be the best value for the humidity of a microscope room? One
manufacturer recommended as low as possible, but we had some bad experiences
with electrostatic charges building up when humidity is low. Zeiss has a
requirement in their specs which is < 65% relative humidity, the comfort
zone for homes should be 30-60%, any ideas?

best wishes

Andreas
Craig Brideau Craig Brideau
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Re: Humidity in microscope rooms

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We use 20-40%.
Below 20% and you get quite a bit of static electricity.
Below 40% and I can modelock a Ti:Saph laser without having to purge the
laser cavity.  Generally our laser systems seem to behave well under 40%.

Craig


On Tue, Dec 14, 2010 at 4:33 AM, Andreas Bruckbauer <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
> what would be the best value for the humidity of a microscope room? One
> manufacturer recommended as low as possible, but we had some bad
> experiences
> with electrostatic charges building up when humidity is low. Zeiss has a
> requirement in their specs which is < 65% relative humidity, the comfort
> zone for homes should be 30-60%, any ideas?
>
> best wishes
>
> Andreas
>
EricMarino EricMarino
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Re: Humidity in microscope rooms

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We keep our rooms at 30%


On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
> what would be the best value for the humidity of a microscope room? One
> manufacturer recommended as low as possible, but we had some bad experiences
> with electrostatic charges building up when humidity is low. Zeiss has a
> requirement in their specs which is < 65% relative humidity, the comfort
> zone for homes should be 30-60%, any ideas?
>
> best wishes
>
> Andreas
>


Eric Marino
Senior Imaging Specialist
Immune Disease Institute
Harvard Medical School
200 Longwood Ave
WAB 133D
Boston, MA 02115
Lab: 617 713-8885
Cell: 617 913-9647
[hidden email]
Eric Marino
Senior Imaging Specialist
Program in Cellular and Molecular Medicine
Boston Children's Hospital
Craig Brideau Craig Brideau
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Re: Humidity in microscope rooms

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Eric, what do you use to control your rooms?  Is it built into the
ventilation system or is it something else you had installed afterwards?

Craig


On Sun, Dec 19, 2010 at 5:34 PM, Eric Marino <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We keep our rooms at 30%
>
>
> On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi,
> > what would be the best value for the humidity of a microscope room? One
> > manufacturer recommended as low as possible, but we had some bad
> experiences
> > with electrostatic charges building up when humidity is low. Zeiss has a
> > requirement in their specs which is < 65% relative humidity, the comfort
> > zone for homes should be 30-60%, any ideas?
> >
> > best wishes
> >
> > Andreas
> >
>
>
> Eric Marino
> Senior Imaging Specialist
> Immune Disease Institute
> Harvard Medical School
> 200 Longwood Ave
> WAB 133D
> Boston, MA 02115
> Lab: 617 713-8885
> Cell: 617 913-9647
> [hidden email]
>
Dan Focht Dan Focht
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Re: Humidity in microscope rooms

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Question;

I did not know about the spec for <65% humidity but it raises a  
question in my mind.
If that is the case, how do you justify pumping warm humidified air  
into a boxed microscope?
In order to prevent surface evaporation, thus osmolarity change, the  
humidity in the box would have to be at or nearly 100%!
Am I missing something here?  Nothing implied, just asking!

Dan


On Dec 19, 2010, at 7:34 PM, Eric Marino wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We keep our rooms at 30%
>
>
> On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi,
>> what would be the best value for the humidity of a microscope  
>> room? One
>> manufacturer recommended as low as possible, but we had some bad  
>> experiences
>> with electrostatic charges building up when humidity is low. Zeiss  
>> has a
>> requirement in their specs which is < 65% relative humidity, the  
>> comfort
>> zone for homes should be 30-60%, any ideas?
>>
>> best wishes
>>
>> Andreas
>>
>
>
> Eric Marino
> Senior Imaging Specialist
> Immune Disease Institute
> Harvard Medical School
> 200 Longwood Ave
> WAB 133D
> Boston, MA 02115
> Lab: 617 713-8885
> Cell: 617 913-9647
> [hidden email]

Dan Focht
[hidden email]
Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16002
www.bioptechs.com
Direct 724-282-7145
Fax 724-282-0745
Toll Free 877 LIVE-CELL - (548-3235)
Aryeh Weiss Aryeh Weiss
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Re: Humidity in microscope rooms

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With our LIS scope incubator, the humidified air is only pumped into the
small cover on the stage, not the entire heated box. Also, when the
sample is removed, if the humidified air is left to flow (eg if we want
to maintain the environment between runs) we put a dummy plate in place
of the sample so that the objective will not have humidified air with 5%
CO2 streaming onto them.

--aryeh

On 12/20/10 5:24 AM, Dan Focht wrote:

>
> Question;
>
> I did not know about the spec for <65% humidity but it raises a question
> in my mind.
> If that is the case, how do you justify pumping warm humidified air into
> a boxed microscope?
> In order to prevent surface evaporation, thus osmolarity change, the
> humidity in the box would have to be at or nearly 100%!
> Am I missing something here? Nothing implied, just asking!
>
> Dan
>
>
> On Dec 19, 2010, at 7:34 PM, Eric Marino wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> We keep our rooms at 30%
>>
>>
>> On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hi,
>>> what would be the best value for the humidity of a microscope room? One
>>> manufacturer recommended as low as possible, but we had some bad
>>> experiences
>>> with electrostatic charges building up when humidity is low. Zeiss has a
>>> requirement in their specs which is < 65% relative humidity, the comfort
>>> zone for homes should be 30-60%, any ideas?
>>>
>>> best wishes
>>>
>>> Andreas
>>>
>>
>>
>

--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051
Andreas Bruckbauer Andreas Bruckbauer
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Re: Humidity in microscope rooms

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Thanks for all the comments about the humidity in microscope rooms, 30%
seems to be a good compromise.

We have currently a room which has to be maintained at 55% and the all in
one box Ti:Sapphire lasers run well, just have to change the purge filter
once a year.

best wishes

Andreas