Andreas Bruckbauer |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, what would be the best value for the humidity of a microscope room? One manufacturer recommended as low as possible, but we had some bad experiences with electrostatic charges building up when humidity is low. Zeiss has a requirement in their specs which is < 65% relative humidity, the comfort zone for homes should be 30-60%, any ideas? best wishes Andreas |
Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We use 20-40%. Below 20% and you get quite a bit of static electricity. Below 40% and I can modelock a Ti:Saph laser without having to purge the laser cavity. Generally our laser systems seem to behave well under 40%. Craig On Tue, Dec 14, 2010 at 4:33 AM, Andreas Bruckbauer <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, > what would be the best value for the humidity of a microscope room? One > manufacturer recommended as low as possible, but we had some bad > experiences > with electrostatic charges building up when humidity is low. Zeiss has a > requirement in their specs which is < 65% relative humidity, the comfort > zone for homes should be 30-60%, any ideas? > > best wishes > > Andreas > |
In reply to this post by Andreas Bruckbauer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We keep our rooms at 30% On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi, > what would be the best value for the humidity of a microscope room? One > manufacturer recommended as low as possible, but we had some bad experiences > with electrostatic charges building up when humidity is low. Zeiss has a > requirement in their specs which is < 65% relative humidity, the comfort > zone for homes should be 30-60%, any ideas? > > best wishes > > Andreas > Eric Marino Senior Imaging Specialist Immune Disease Institute Harvard Medical School 200 Longwood Ave WAB 133D Boston, MA 02115 Lab: 617 713-8885 Cell: 617 913-9647 [hidden email]
Eric Marino
Senior Imaging Specialist Program in Cellular and Molecular Medicine Boston Children's Hospital |
Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Eric, what do you use to control your rooms? Is it built into the ventilation system or is it something else you had installed afterwards? Craig On Sun, Dec 19, 2010 at 5:34 PM, Eric Marino <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We keep our rooms at 30% > > > On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi, > > what would be the best value for the humidity of a microscope room? One > > manufacturer recommended as low as possible, but we had some bad > experiences > > with electrostatic charges building up when humidity is low. Zeiss has a > > requirement in their specs which is < 65% relative humidity, the comfort > > zone for homes should be 30-60%, any ideas? > > > > best wishes > > > > Andreas > > > > > Eric Marino > Senior Imaging Specialist > Immune Disease Institute > Harvard Medical School > 200 Longwood Ave > WAB 133D > Boston, MA 02115 > Lab: 617 713-8885 > Cell: 617 913-9647 > [hidden email] > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Question; I did not know about the spec for <65% humidity but it raises a question in my mind. If that is the case, how do you justify pumping warm humidified air into a boxed microscope? In order to prevent surface evaporation, thus osmolarity change, the humidity in the box would have to be at or nearly 100%! Am I missing something here? Nothing implied, just asking! Dan On Dec 19, 2010, at 7:34 PM, Eric Marino wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We keep our rooms at 30% > > > On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi, >> what would be the best value for the humidity of a microscope >> room? One >> manufacturer recommended as low as possible, but we had some bad >> experiences >> with electrostatic charges building up when humidity is low. Zeiss >> has a >> requirement in their specs which is < 65% relative humidity, the >> comfort >> zone for homes should be 30-60%, any ideas? >> >> best wishes >> >> Andreas >> > > > Eric Marino > Senior Imaging Specialist > Immune Disease Institute > Harvard Medical School > 200 Longwood Ave > WAB 133D > Boston, MA 02115 > Lab: 617 713-8885 > Cell: 617 913-9647 > [hidden email] Dan Focht [hidden email] Bioptechs, Inc. 3560 Beck Rd. Butler, PA 16002 www.bioptechs.com Direct 724-282-7145 Fax 724-282-0745 Toll Free 877 LIVE-CELL - (548-3235) |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** With our LIS scope incubator, the humidified air is only pumped into the small cover on the stage, not the entire heated box. Also, when the sample is removed, if the humidified air is left to flow (eg if we want to maintain the environment between runs) we put a dummy plate in place of the sample so that the objective will not have humidified air with 5% CO2 streaming onto them. --aryeh On 12/20/10 5:24 AM, Dan Focht wrote: > > Question; > > I did not know about the spec for <65% humidity but it raises a question > in my mind. > If that is the case, how do you justify pumping warm humidified air into > a boxed microscope? > In order to prevent surface evaporation, thus osmolarity change, the > humidity in the box would have to be at or nearly 100%! > Am I missing something here? Nothing implied, just asking! > > Dan > > > On Dec 19, 2010, at 7:34 PM, Eric Marino wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> We keep our rooms at 30% >> >> >> On Dec 14, 2010, at 6:33 AM, Andreas Bruckbauer wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Hi, >>> what would be the best value for the humidity of a microscope room? One >>> manufacturer recommended as low as possible, but we had some bad >>> experiences >>> with electrostatic charges building up when humidity is low. Zeiss has a >>> requirement in their specs which is < 65% relative humidity, the comfort >>> zone for homes should be 30-60%, any ideas? >>> >>> best wishes >>> >>> Andreas >>> >> >> > -- Aryeh Weiss School of Engineering Bar Ilan University Ramat Gan 52900 Israel Ph: 972-3-5317638 FAX: 972-3-7384051 |
Andreas Bruckbauer |
In reply to this post by Andreas Bruckbauer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thanks for all the comments about the humidity in microscope rooms, 30% seems to be a good compromise. We have currently a room which has to be maintained at 55% and the all in one box Ti:Sapphire lasers run well, just have to change the purge filter once a year. best wishes Andreas |
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