Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Confocalists, For many reasons it seemed to me a good idea to create an image explorer program (like Windows Explorer) that would allow you to see thumbnail images of proprietary confocal file formats, in addition to standard image file formats. I suggested the idea to one of our Bioinformtics guys and he's written a program which he's called FocalPoint. At this point you may well tell me this is reinventing the wheel, but to date, I haven't seen anything quite like this. The program displays thumbnail images of Zeiss (.lsm) and Olympus (.oib, .oif) confocal images files in addition to standard image file formats (avi, jpg, tiff, bmp, png). If the image file contains multiple channels or images acquired over depth or time then slider bars appear adjacent to the thumbnail image and allow you to navigate through the stack. You can also easily resize the thumbnail images to allow you to see more or less detail as required. If there are people out there who think they may benefit from this software then it can be freely downloaded here (PC and Mac): http://www.bioinformatics.bbsrc.ac.uk/projects/focalpoint/ At the moment the software is still work in progress so there may be one or two bugs. Let me know if you find anything. I'd also be interested to hear if anyone has any suggestions on how the software could be improved. Currently there is support only for Zeiss and Olympus confocal file formats as these are the instruments we have here. If anyone would like support added for other common confocal file formats (eg .lei) then perhaps you could send me a few small sample files and we'll see what we can do. Simon Walker Babraham Institute Cambridge UK CB22 3AT |
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
I only have one request : would it be possible to support .stk Metamorph stacks (built-in in ImageJ) and .lei/.lif leica files (by using the LOCI tools importer API) ?
Christophe On Mon, Apr 21, 2008 at 11:17 AM, Simon Walker <[hidden email]> wrote: Search the CONFOCAL archive at |
In reply to this post by simon walker (BI)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear Simon, this will be a welcome bit of software. I will download it and try it out. Before becoming a Zeiss user, I would catalogue all of my image files into subject specific libraries using Portfolio or comparable software. Various other software handle the Zeiss file format, e.g. Where Is It, but still require a saved separate image to be catalogued for image stacks and time series (correct me if I'm wrong - I usually am). An image organising program very quickly becomes cumbersome if every image from a stack or timeseries is thumbnailed individually. Sliders for navigating through stacks sounds like a perfect solution. Have your colleague keep up the good work. Regards, John. Simon Walker wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Confocalists, For many reasons it seemed to me a good idea to create an image explorer program (like Windows Explorer) that would allow you to see thumbnail images of proprietary confocal file formats, in addition to standard image file formats. I suggested the idea to one of our Bioinformtics guys and he's written a program which he's called FocalPoint. At this point you may well tell me this is reinventing the wheel, but to date, I haven't seen anything quite like this. The program displays thumbnail images of Zeiss (.lsm) and Olympus (.oib, .oif) confocal images files in addition to standard image file formats (avi, jpg, tiff, bmp, png). If the image file contains multiple channels or images acquired over depth or time then slider bars appear adjacent to the thumbnail image and allow you to navigate through the stack. You can also easily resize the thumbnail images to allow you to see more or less detail as required. If there are people out there who think they may benefit from this software then it can be freely downloaded here (PC and Mac): http://www.bioinformatics.bbsrc.ac.uk/projects/focalpoint/ At the moment the software is still work in progress so there may be one or two bugs. Let me know if you find anything. I'd also be interested to hear if anyone has any suggestions on how the software could be improved. Currently there is support only for Zeiss and Olympus confocal file formats as these are the instruments we have here. If anyone would like support added for other common confocal file formats (eg .lei) then perhaps you could send me a few small sample files and we'll see what we can do. Simon Walker Babraham Institute Cambridge UK CB22 3AT --
*********************************
Visit
The Illuminated Plant
Cell
dot com |
In reply to this post by lechristophe
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are very interested in having additional applications utilize the Bio-formats library (http://www.loci.wisc.edu/ome/formats.html). By doing so your application would support the 55 plus formats that Bio-formats can currently open. If you are interested in doing this, please contact us offlist and we can help. best, kevin Kevin W. Eliceiri Director Laboratory for Optical and Computational Instrumentation http://www.loci.wisc.edu Room 271 Animal Sciences 1675 Observatory Drive Madison, WI 53706 Phone: 608-263-6288 Fax: 608-262-4570 ----- Original Message ----- From: Christophe Leterrier <[hidden email]> Date: Tuesday, April 22, 2008 2:24 am Subject: Re: Image Explorer To: [hidden email] > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > I only have one request : would it be possible to support .stk Metamorph > stacks (built-in in ImageJ) and .lei/.lif leica files (by using the LOCI > tools importer API) ? > > Christophe > > On Mon, Apr 21, 2008 at 11:17 AM, Simon Walker <[hidden email]> > wrote: > > > Search the CONFOCAL archive at > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > > Dear Confocalists, > > > > For many reasons it seemed to me a good idea to create an image explorer > > program (like Windows Explorer) that would allow you to see thumbnail > > images > > of proprietary confocal file formats, in addition to standard image > file > > formats. > > I suggested the idea to one of our Bioinformtics guys and he's > written a > > program which he's called FocalPoint. At this point you may well > tell me > > this is > > reinventing the wheel, but to date, I haven't seen anything quite like > > this. > > > > The program displays thumbnail images of Zeiss (.lsm) and Olympus (.oib, > > .oif) > > confocal images files in addition to standard image file formats (avi, > > jpg, tiff, > > bmp, png). If the image file contains multiple channels or images > > acquired > > over depth or time then slider bars appear adjacent to the thumbnail > image > > and allow you to navigate through the stack. You can also easily resize > > the > > thumbnail images to allow you to see more or less detail as required. > > > > If there are people out there who think they may benefit from this > > software > > then it can be freely downloaded here (PC and Mac): > > http://www.bioinformatics.bbsrc.ac.uk/projects/focalpoint/ > > > > At the moment the software is still work in progress so there may be > one > > or > > two bugs. Let me know if you find anything. I'd also be interested > to > > hear if > > anyone has any suggestions on how the software could be improved. > > Currently there is support only for Zeiss and Olympus confocal file > > formats as > > these are the instruments we have here. If anyone would like support > > added > > for other common confocal file formats (eg .lei) then perhaps you could > > send > > me a few small sample files and we'll see what we can do. > > > > Simon Walker > > Babraham Institute > > Cambridge > > UK > > CB22 3AT > > |
Free forum by Nabble | Edit this page |