Image Scanning Microscopy (ISM) - practical formulas?

[Reece, Jeff (NIH/NIDDK) [E]-2]

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Dear List,

I am trying to wrap my brain around the theoretical framework behind ISM and its current commercial implementations.  I have looked at a few papers discussing theory (Colin Sheppard obviously the biggest contributor in this respect).  While helpful, I have yet to find answers to these questions:


  1.  Are there formulas which give: a) peak brightness relative to regular confocal 1AU, b) xy resolution, and c) z resolution; as a function of NA, wavelength, and applied pinhole size (anything else?)?
  2.  For the Yokogawa SoRa implementation, how are such formulas altered by: a) optically coupling the pinhole after the "optical reassignment"; b) the intermediate "magnification changer"; and c) objective lens mag?

Looking forward to any discussion.
Stay Safe and Healthy,
Jeff

Jeff Reece
Ph: +1.301.451.4330
E:  [hidden email]<https://mail.nih.gov/owa/14.3.174.1/scripts/premium/redir.aspx?C=vorg2hwQ3EG79HF4VARC2_-txi1AZNEITAaQhKx2WUBLeDOG3BM2dSsWeRsCBbyhbstXsPzU2G8.&URL=mailto%3ajeff.reece%40nih.gov>

Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC)
NIH/NIDDK
Bethesda, MD  20892

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