ImageExpress IXM losing its focus

classic Classic list List threaded Threaded
1 message Options
Axel Kurt Preuss Axel Kurt Preuss
Reply | Threaded
Open this post in threaded view
|

ImageExpress IXM losing its focus


Dear All
I shall "reflect" on Image Express s (IXM) problem of losing it's focus quite literally

At some point during scanning wells (autofocus on)  it starts racing and loses focus . (Windows shows application hang in it's event log at that time, we didn't have Axotracer switched on, unfortunately)
On another note It also tends to lose focus in an irregular pattern one of four fields acquired within wells . Changing exposure time in Configure Autofocus setting indicates that wells out of focus were not able to collect 2nd focus score peak. But extending exposure to focus beam (did indeed bring these wells back into focus but) did not prevent the behavior above: speed up with concomitant loss of focus
Would anybody be able to dissect this problem with lasersharp focus?
Users tell me system has performed well for almost a year but now  starts to act in this manner.






Thanks
  Cheers

Axel
—————
Axel K Preuss, PhD,
Central Imaging, IMCB, A*Star, 61 Biopolis Dr<x-apple-data-detectors://3>, 6-19B, Singapore 138673,  sent from9271.5622<tel:9271.5622>

Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.