ImageJ Help

[Matiar Jafari]

Dear Confocal List Serv,

I'm looking for a way to draw lines of a set length on ImageJ.  I need
to measure spines on dendrites every 20 um from the soma on
dissociated neurons grown in tissue culture.  I've tried using a
modified Sholl analysis, but without the laminar constraints the
dendrites grow with various curves.  I would like to use the freehand
lines tool to stop at 20 um as I trace along the dendrite.

Thank You

[Guillermo Palchik]

Matiar,

Did you try the Sholl plugin for ImageJ?
Also, there is an ImageJ Listserv (https://list.nih.gov/archives/imagej.html). You should post your question there...
Best,
Gil

On Dec 3, 2009, at 6:46 PM, Matiar Jafari wrote:

> Dear Confocal List Serv,
>
> I'm looking for a way to draw lines of a set length on ImageJ.  I need
> to measure spines on dendrites every 20 um from the soma on
> dissociated neurons grown in tissue culture.  I've tried using a
> modified Sholl analysis, but without the laminar constraints the
> dendrites grow with various curves.  I would like to use the freehand
> lines tool to stop at 20 um as I trace along the dendrite.
>
> Thank You

--
Guillermo Palchik
Ph.D. Candidate - Interdisciplinary Program in Neuroscience
Georgetown University Medical Center
Research Building Room W 217
3970 Reservoir Rd. NW, Washington, DC 20007
Lab: 202-687-7825

[B. Prabhakar Pandian]

Hello,
           Can anybody direct me to a good paper on pH measurements
(intracellular and extracellular) using SNARF especially for neuronal cells.

Thanks,

-Prabhakar

--

[Lemasters, John J.]

You might want to look at:

Chacon, E., J.M. Reece, A.-L. Nieminen, G. Zahrebelski, B. Herman, and J.J. Lemasters (1994) Distribution of electrical potential, pH, free Ca2+, and volume inside cultured adult rabbit cardiac myocytes during chemical hypoxia: a multiparameter digitized confocal mi-croscopic study.  Biophys. J. 66, 942-952.

John

--
John J. Lemasters, MD, PhD
Professor and South Carolina COEE Endowed Chair
Director, Center for Cell Death, Injury and Regeneration
Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology
Medical University of South Carolina
QF213 Quadrangle Building
280 Calhoun Street, MSC 140
Charleston, SC 29425

Office: 843-792-2153
Lab: 843-792-3530
Fax: [phone]
Email: [hidden email]


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian
Sent: Friday, December 04, 2009 3:56 PM
To: [hidden email]
Subject: pH measurement using SNARF

Hello,
           Can anybody direct me to a good paper on pH measurements
(intracellular and extracellular) using SNARF especially for neuronal cells.

Thanks,

-Prabhakar

--
No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 8.5.426 / Virus Database: 270.14.91/2541 - Release Date: 12/02/09 19:43:00

[B. Prabhakar Pandian]

Thanks. I will take a look at this paper.

-Prabhakra

Lemasters, John wrote:

> You might want to look at:
>
> Chacon, E., J.M. Reece, A.-L. Nieminen, G. Zahrebelski, B. Herman, and J.J. Lemasters (1994) Distribution of electrical potential, pH, free Ca2+, and volume inside cultured adult rabbit cardiac myocytes during chemical hypoxia: a multiparameter digitized confocal mi-croscopic study.  Biophys. J. 66, 942-952.
>
> John
>
> --
> John J. Lemasters, MD, PhD
> Professor and South Carolina COEE Endowed Chair
> Director, Center for Cell Death, Injury and Regeneration
> Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology
> Medical University of South Carolina
> QF213 Quadrangle Building
> 280 Calhoun Street, MSC 140
> Charleston, SC 29425
>
> Office: 843-792-2153
> Lab: 843-792-3530
> Fax: [phone]
> Email: [hidden email]
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian
> Sent: Friday, December 04, 2009 3:56 PM
> To: [hidden email]
> Subject: pH measurement using SNARF
>
> Hello,
>            Can anybody direct me to a good paper on pH measurements
> (intracellular and extracellular) using SNARF especially for neuronal cells.
>
> Thanks,
>
> -Prabhakar
>
>  

--

[Stephen Cody-2]

Dear Prabhakar,

Here are a couple of papers but not with neuronal cells.

Cody, S.H., Dubbin, P.N., Beischer, A., Duncan, N.D., Hill, J., Kaye,
A., & Williams, D.A. Intracellular pH mapping with Snarf-1 and
confocal microscopy. I: A quantitative technique for living cells and
isolated tissues. Micron 24(6): 573-580, (1993).
http://dx.doi.org/10.1016/0968-4328(93)90034-X

Dubbin, P.N., Cody, S.H. & Williams, D.A. Intracellular pH mapping
with Snarf-1 and confocal microscopy. II: pH gradients within single
cultured cells. Micron 24(6): 581-586, (1993).
http://dx.doi.org/10.1016/0968-4328(93)90035-Y

Cheers Stephen Cody

2009/12/5 B. Prabhakar Pandian <[hidden email]>:

> Hello,
>          Can anybody direct me to a good paper on pH measurements
> (intracellular and extracellular) using SNARF especially for neuronal cells.
>
> Thanks,
>
> -Prabhakar
>
> --
>

--
Stephen H. Cody

[Norbert Opitz]

B. Prabhakar Pandian wrote:
> Hello,
>           Can anybody direct me to a good paper on pH measurements
> (intracellular and extracellular) using SNARF especially for neuronal
> cells.
>
> Thanks,
>
> -Prabhakar
>

Maybe our paper will be of interest too :

Opitz,N., Merten,E.,Acker,H., Pflugers Archiv - European Journal of
Physiology. 427(3-4):332-342, 1994
EVIDENCE FOR REDISTRIBUTION-ASSOCIATED INTRACELLULAR PK SHIFTS OF THE
PH-SENSITIVE FLUOROPROBE CARBOXY-SNARF-1.