ImageJ course London 8-9 May

[Moulding, Dale]

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Dear list,

I've been running a 2 day ImageJ course for a few years now and would like to open this up for wider attendance.
Course details and link to booking:
https://www.ucl.ac.uk/child-health/core-scientific-facilities-centres/confocal-microscopy/training-courses

Anonymous user feedback (over 200 attendees, 99 responses) gives a 100% recommended score, and you can read anonymous user comments here:
https://docs.google.com/forms/d/1zpZ-exLM9IJ2SB02OE6YM2zbmitElk2916Pow4Qgcbo/viewanalytics

Fees are : £50 UCL, £75 other academic, £100 non-academic. Free for ICH imaging facility users.

I run the course every 3 months.

Cheers

Dale
Dale Moulding PhD FRMS
ICH Light Microscopy Facility
UCL Great Ormond Street Institute of Child Health
30 Guilford St
London WC1N 1EH
Mob: [phone]
Tel: [phone]
Please acknowledge use of the BRC funded facility in all publications:
http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy

[Arvydas Matiukas]

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Dear list,

We are looking for the second confocal for our Microscopy Core that would be exclusively used to image fixed samples (ranging from cells to tissue slices <500um, wide range of dyes and fluorescent proteins, up to 4 colors). Highest image quality (for grants, publications) still is our primary  goal, however some parameters like acquisition speed, detector number and sensitivity does not need to be the absolutely best. Freely adjustable detection bands for all detectors would be a plus.

Please advise on the confocal model and configuration and/or share what you are using for imaging fixed samples.

We plan applying for the shared instrumentation grant so price up to $500k is fine.


Best regards,
Arvydas


+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Email: [hidden email]

[Vincent RENAUD]

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Dear Arvydas,

I am working for Axiom Optics and we represent Confocal.nl<http://Confocal.nl> here in the US so I do have commercial interest.

The Rescan Confocal Microscope is an add-on to a widefield inverted frame microscope (such as Nikon Ti2, Olympus IX, Leica DMi8, …) which brings laser scanning capability. The main difference compare to any other LSM is that it is camera-based instead of PMT-based which offers 3-4X more sensitivity and 170nm lateral resolution RAW data (please go here to learn more about the rescan technology: http://www.confocal.nl/explanation/ ).

In few points this system is super easy to use (literally 3 buttons to click on before getting a 170nm lateral res image) so very little training for your users, offers anything a regular widefield stand would offer (tiling capabilities, z-stacking, etc …), is integrated in NIS-Elements as well as Micro-Manager, and needs very little to no support (no motorization in the RCM add-on).

Price point is ~ 200 - 250 KUSD for the whole solution including the RCM add-on, a 4 laser-lines combiner, a sCMOS camera, a Nikon Ti2-E stand with objective lenses, filters, etc … If you already have a scope it is less than 90 KUSD but I believe this is not what you are looking for.

Please feel free to contact me if you want to keep discussing this: [hidden email]<mailto:[hidden email]> .
Good luck for your research,
Best,

Vincent Renaud
Sales Application Engineer
[phone]

On May 2, 2019, at 11:40 AM, Arvydas Matiukas <[hidden email]<mailto:[hidden email]>> wrote:

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Dear list,

We are looking for the second confocal for our Microscopy Core that would be exclusively used to image fixed samples (ranging from cells to tissue slices <500um, wide range of dyes and fluorescent proteins, up to 4 colors). Highest image quality (for grants, publications) still is our primary  goal, however some parameters like acquisition speed, detector number and sensitivity does not need to be the absolutely best. Freely adjustable detection bands for all detectors would be a plus.

Please advise on the confocal model and configuration and/or share what you are using for imaging fixed samples.

We plan applying for the shared instrumentation grant so price up to $500k is fine.


Best regards,
Arvydas


+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Email: [hidden email]

[Craig Brideau]

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Hi Arvydas, 'fixed samples' covers a wide variety of possibilities. In my
own experience, for thin samples the sample preparation quality is much
more important than the imaging system. This also holds true for thicker
samples, but in general for confocal of thick specimens, longer wavelengths
are better. This means you would be looking for something that has red and
far red capabilities.
Finally, depending on exactly what you are working with, a good tissue
clearing system might be worthwhile.

Best of luck!
Craig

On Thu, May 2, 2019 at 1:01 PM Arvydas Matiukas <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
>
> We are looking for the second confocal for our Microscopy Core that would
> be exclusively used to image fixed samples (ranging from cells to tissue
> slices <500um, wide range of dyes and fluorescent proteins, up to 4
> colors). Highest image quality (for grants, publications) still is our
> primary  goal, however some parameters like acquisition speed, detector
> number and sensitivity does not need to be the absolutely best. Freely
> adjustable detection bands for all detectors would be a plus.
>
> Please advise on the confocal model and configuration and/or share what
> you are using for imaging fixed samples.
>
> We plan applying for the shared instrumentation grant so price up to $500k
> is fine.
>
>
> Best regards,
> Arvydas
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Email: [hidden email]
>

[Darran Clements]

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To join, leave or search the confocal microscopy listserv, go to:
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*****

Hi Arvydas
We are currently demoing an Aurox Clarity laser free confocal system. It's been very pleasantly surprising in its performance.
The Clarity unit has 4 colours, easy user replaceable filter cubes, a choice of stands, cameras, laser sources to suit budget/availability in your lab, so plenty of tailoring is possible as well as upgrading to appropriate filters when the need arises.
It's a quick system, and we have found that it performs far better at lower magnifications for embryos etc than our existing spinning disk system, it's also very easy to pick up and train users on.
It does a large proportion of what a point scanner can do for the vast majority of imaging use cases and users.

The one we have has an Orca Flash 4 camera and the sensitivity is pretty. It's been used to image 4 colour labelled fixed and live embryo samples, cells, thick tissue sections and even some confetti samples, as well as a few tests of how it performs for SRRF superresolution imaging and it's been impressive.
Well worth a look, the price for the performance is very, very attractive.
They also have an all in one box system in development called the Unity, and as we do quite a lot of plate scanning and regularly image 5 or 6 colour samples, the 8 channels that will have is also something to seriously consider.
When you also consider purchases as part of a strategic plan to extend capabilities in facilities, systems like this have the possibility of providing quick, effective and excellent functionality whilst liberating other systems to be more specialised and used for tasks they are better suited to, instead of the situation we've all seen where users try and get on the latest, greatest systems to do very routine 2 channel imaging.

Darran
Stem Cell Institute
Cambridge

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas
Sent: 02 May 2019 19:41
To: [hidden email]
Subject: Best confocal for fixed samples

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Dear list,

We are looking for the second confocal for our Microscopy Core that would be exclusively used to image fixed samples (ranging from cells to tissue slices <500um, wide range of dyes and fluorescent proteins, up to 4 colors). Highest image quality (for grants, publications) still is our primary  goal, however some parameters like acquisition speed, detector number and sensitivity does not need to be the absolutely best. Freely adjustable detection bands for all detectors would be a plus.

Please advise on the confocal model and configuration and/or share what you are using for imaging fixed samples.

We plan applying for the shared instrumentation grant so price up to $500k is fine.


Best regards,
Arvydas


+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Email: [hidden email]

[Avi Jacob]

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*****

That's a nice budget. Don't see why you shouldn't go for a mid-high end
system. Maybe a low configured high-end system. I currently have Leica (my
Zeiss' and Olympus' are old so I can't compare.)
Were you to get an SP8, with say 3 detectors (at least one HyD) and a white
light laser with gating (I love gating, not only for SR), you are covering
all bases. We are very pleased with ours (4 years now  - though ours is a
bit higher end, with a galvo secondary stage, 4 detectors, 3D STED).
You can upgrade later to Falcon, STED etc.
Avi

--
Avi Jacob, Ph.D.
Head of The Kanbar Light Microscopy Unit
The Mina & Everard Goodman Faculty of Life Sciences
Bar-Ilan University, Ramat-Gan 5290002, Israel




On Fri, May 3, 2019 at 12:07 PM Darran Clements <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Arvydas
> We are currently demoing an Aurox Clarity laser free confocal system. It's
> been very pleasantly surprising in its performance.
> The Clarity unit has 4 colours, easy user replaceable filter cubes, a
> choice of stands, cameras, laser sources to suit budget/availability in
> your lab, so plenty of tailoring is possible as well as upgrading to
> appropriate filters when the need arises.
> It's a quick system, and we have found that it performs far better at
> lower magnifications for embryos etc than our existing spinning disk
> system, it's also very easy to pick up and train users on.
> It does a large proportion of what a point scanner can do for the vast
> majority of imaging use cases and users.
>
> The one we have has an Orca Flash 4 camera and the sensitivity is pretty.
> It's been used to image 4 colour labelled fixed and live embryo samples,
> cells, thick tissue sections and even some confetti samples, as well as a
> few tests of how it performs for SRRF superresolution imaging and it's been
> impressive.
> Well worth a look, the price for the performance is very, very attractive.
> They also have an all in one box system in development called the Unity,
> and as we do quite a lot of plate scanning and regularly image 5 or 6
> colour samples, the 8 channels that will have is also something to
> seriously consider.
> When you also consider purchases as part of a strategic plan to extend
> capabilities in facilities, systems like this have the possibility of
> providing quick, effective and excellent functionality whilst liberating
> other systems to be more specialised and used for tasks they are better
> suited to, instead of the situation we've all seen where users try and get
> on the latest, greatest systems to do very routine 2 channel imaging.
>
> Darran
> Stem Cell Institute
> Cambridge
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Arvydas Matiukas
> Sent: 02 May 2019 19:41
> To: [hidden email]
> Subject: Best confocal for fixed samples
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
>
> We are looking for the second confocal for our Microscopy Core that would
> be exclusively used to image fixed samples (ranging from cells to tissue
> slices <500um, wide range of dyes and fluorescent proteins, up to 4
> colors). Highest image quality (for grants, publications) still is our
> primary  goal, however some parameters like acquisition speed, detector
> number and sensitivity does not need to be the absolutely best. Freely
> adjustable detection bands for all detectors would be a plus.
>
> Please advise on the confocal model and configuration and/or share what
> you are using for imaging fixed samples.
>
> We plan applying for the shared instrumentation grant so price up to $500k
> is fine.
>
>
> Best regards,
> Arvydas
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Email: [hidden email]
>

[Matthias Reuss]

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*****

Dear Arvydas,

I am one of the main developers of the Facility Line microscope https://www.abberior-instruments.com/products/facility-line/ at Abberior Instruments (so please note I have a commercial interest).

Regarding your question, you may want to have a look at our recently announced Facility Line microscope which covers all of your requested confocal microscopy needs. Highlights include superb confocal image quality with spectrally tunable detection, full auto-alignment (all lasers and pinhole), and adaptive illumination schemes for significantly reduced photobleaching of all existing fluorescent markers.

On top, you get cutting-edge STED superresolution down to a resolution of 25 nm.

The system is operated with a super-easy to learn software which we have designed from scratch, particularly for multiuser environments in facilities.

If you happen to attend the ELMI conference in Brno (Czech Republic) on June 4-7, we demo the Facility line live there. Otherwise, feel free to get in touch with my colleagues in the US ([hidden email]) any time for further information.



Best

-Matthias



______________________________

Dr. Matthias Reuss

Head of Research & Development

Abberior Instruments GmbH

Mail:[hidden email]