Imaging Course - Second Announcement

[Hard, Robert]

 

This is the SECOND announcement for the imaging course:

 

Title: Optical Microscopy and Imaging in the Biomedical Sciences

 

When: October 5 - October 14, 2010

 

Where: Marine Biology Laboratory, Woods Hole, MA, USA

 

Tuition: $2800 (Includes room and board, text, handouts, supplies)

 

Application Deadline: Applications accepted through June 25, 2010

 

Admission application and information:

Carol Hamel, Admissions Coordinator

Marine Biological Laboratory

7 MBL Street

Woods Hole, MA 02543-1015

[phone]

Internet: [hidden email]

WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

 

Course Directors:

Robert Hard, University at Buffalo

Phone: [716] 829-3521

Email: [hidden email]

 

Colin S. Izzard, University at Albany

Phone: [518] 439-7121

Email: [hidden email]

 

 

Course Description:

 

This course will utilize the NEWLY RENOVATED and MODERNIZED FACILITIES IN THE LOEB BLDG AT THE MARINE BIOLOGY LABORATORy.

 

For Whom:

Designed primarily for research scientists, postdoctoral trainees and advanced graduate students in animal, plant, medical and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and digital imaging will benefit greatly from this course. There are no specific prerequisites, but an understanding of the basic principles of optics and imaging is desirable. The course is limited to 26 students.

 

Course Organization:

The course consists of lectures, hands-on laboratory exercises, problem sets, laboratory demonstrations and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce digital records for documentation and analysis. In the problem sets, small groups of students will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopy and imaging skills.

 

Live and fixed specimens will be provided for use in laboratory exercises and demonstrations.

 

HOWEVER, STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (CELL CULTURES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS TO USE THROUGHOUT THE COURSE FOR EXERCISES, WHERE APPROPRIATE. Cell culture facilities are available for student use. STUDENTS ALSO ARE ENCOURAGED TO MAKE APPOINTMENTS WITH OUR EXPERIENCED COMMERCIAL FACULTY OUTSIDE OF SCHEDULED CLASSES TO ACQUIRE DATA FROM THEIR SPECIMENS USING STATE OF THE ART EQUIPMENT AVAILABLE. Students are highly encouraged to discuss their individual research problems with the academic and commercial faculty.

 

Topics To Be Covered Include:

 

Principles of microscope design and image formation

 

Bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy

 

Digital imaging, recording, enhancement, processing and analysis

 

Advanced fluorescent techniques (eg. FRAP, FLIP, FRET, FLIM, fluorescence polarization,  multi-spectral imaging)

 

Confocal scanning microscopy (LSM and spinning disc), multiphoton excitation fluorescence microscopy and structured illumination systems

 

Image deconvolution, digital image restoration

 

3D image acquisition, display, and analysis

 

Live cell imaging

 

Laser microdissection and microcapture of tissues/cells for molecular biology.

 

Super-resolution techniques (4PI, STED, PALM, etc.)

 

Application of optical methods to live cells will be emphasized; other specimens will be covered.

 

Students will have direct hands-on experience with state-of-the-art microscopes, digital cameras, spectrophotometers, and image acquisition/processing/analysis software provided by major optical, electronics, and software companies. Instruction will be provided by experienced staff from universities and industry.

 

 

Recent faculty have included:

 

Joseph A DePasquale, Morphogenyx

Shinya Inoue, Marine Biology Laboratory

James McNally, National Cancer Institute, NIH

Butch Moomaw, Hamamatsu

John M. Murray, University of Pennsylvania School of Medicine

Rudolph Oldenberg, Marine Biology Laboratory

David Piston, Vanderbilt University

Wade Sigurdson, University at Buffalo

Clare M. Waterman, National Heart Lung and Blood Institute, NIH

 

 

See Also:

http://www.mbl.edu/education/courses/special_topics/om.html