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Hi Jean-Pierre
I don't know about Imaris, but we measure the volume in a very simple way, without any membrane markers
J. Microbiol. Methods, 87, 375-377 (2011).
BioTechniques, 50, 389-396 (2011).
Pflugers Arch. 460, 1097-1104 (2010).
There have been some additional developments, not yet published, can send you a paper if interested.
Best,
Mike
-----Original Message-----
From: Confocal Microscopy List [mailto:
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Sent: Thursday, February 16, 2012 7:51 PM
To:
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Subject: Imaris and volume measurement
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Hi,
I was wondering if anyone knows what Imaris volume measurement statistic
means in the case were the volume is not totally closed ?
Indeed I have cells stained with a membrane marker so I can create a 3D
volume of the cell. However the bottom of the cell touching the glass has
areas where there is no staining (or not strong enough). Therfore Imaris
doesn't close the volume.
Thank you
JP
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Jean-Pierre CLAMME, PhD
Chief Scientist
Nitto Denko Technical
501 Via Del Monte
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