Imaris volume and spots rotation

[Ekaterina PAPUSHEVA-2]

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Dear colleagues,



As all sad Imaris users probably know, when you do a rotation of a volume in Imaris, tracks are not rotated.The flaw that we repeatedly report to Bitplane since two years, without feedback...

Now, i am trying to develop an extension, that reads rotation parameters (axes, angle) and the track coordinates from Imaris to Matlab, rotates the tracks, and re-imports rotated result back to Imaris volume.



the volume itself i then rotate via Imaris free rotation, as it is to much data to suck them into matlab, rotate and export- my poor computer refuses to do it.



now, the tracks that i rotate with allegedly the   parameters that "free rotate"  option of Imaris uses, appear completely out of place. it is not just a rotation center displacement, they have completely wrong direction! So it seems Imaris uses some other rotation parameters, then those that are specified in "Free Rotate"!!!



did someone see this problem before? If someone found out, what really Imaris does with its volume when "Free Rotate" is applied, and how to rotate tracks in the same way, we will be really grateful for a share of experience!

Bitplane is not really helpful in this case...



best regards,

Ekaterina


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Ekaterina Papusheva

Manager of Bioimaging Facility

Institute of Science and Technology Austria

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