Immersion Oil
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Hi all,
sorry I am late in the discussion about the oil immersion but I only have a small practical doubt.... How it could be that Cargille oil cost less than half compared with Leica or Zeiss ones? And so where is the starting point where the immersion oil plays a decisive role in your experiment? It depend on the magnification you are working with (and consequently the NA) or it depend on what you are looking for?Is for the confocal acquisition better to buy the "good" one? Thanks in advance Valeria Richard E. Edelmann wrote: > I am going to chime in agreement with bad results from mixing oils. > About six years ago we ran into problems with mixing oils (Fryer oil > which was a repackaged Cargille vs Olympus, etc.) and had severe > issues with resolution degradation. And we found that it took > serious scrupulous cleaning the lenses to remove all contamination. > (No gooy oil or concretions, etc. just hard to scrupulously clean). > > Yes, scrupulously cleaning previous oil from slides "could have > worked" but the fact is users rearely clean the old oils well enough. > > Alternately requiring slides not to be used with oil anywhere else - > > but that also is a trust system. So rather than waste time cleaning > lenses, I simply bought a bunch of new oil and went around and traded > > oil bottle with every lab. Since I was giving them new oil no one > complained. No more mixing issues. > > A little expensive maybe but time and good faith it was worth it. > > > On 17 Mar 2009 at 13:43, Carl Boswell wrote: > > >> The stories are real, at least with respect to the miscibility of >> different oils. While one might think that oil is oil, two different >> immersion oils together can behave like oil and water. That is why I >> insist that samples first viewed on some other microscope be >> scrupulously cleaned of whatever oil was used before applying oil used >> with our Core systems. >> >> As for the issue of one manufacture's oil eating away another >> manufacture's lens, I'd consider the source of that information >> carefully. I also use Cargille oil on multiple sytems and have no >> complaints. C >> >> Carl A. Boswell, Ph.D. >> Molecular and Cellular Biology >> University of Arizona >> 520-954-7053 >> FAX 520-621-3709 >> ----- Original Message ----- >> From: David Knecht<mailto:[hidden email]> >> To: [hidden email]<mailto:[hidden email]> >> Sent: Tuesday, March 17, 2009 6:56 AM >> Subject: Immersion Oil >> >> We now have Nikon, Zeiss and Leica microscopes in our facility. Is the problem of cross-contamination of immersion oil real or hypothetical? Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes? Dave >> >> Dr. David Knecht >> Department of Molecular and Cell Biology >> Co-head Flow Cytometry and Confocal Microscopy Facility >> U-3125 >> 91 N. Eagleville Rd. >> University of Connecticut >> Storrs, CT 06269 >> 860-486-2200 >> 860-486-4331 (fax) >> >> >> > Richard E. Edelmann, Ph.D. > EXPO Editor, Microscopy and Microanalysis Supplement > Electron Microscopy Facility Director > 364 Pearson Hall > Miami University, Oxford, OH 45056 > Ph: 513.529.5712 Fax: 513.529.4243 > E-mail: [hidden email] > http://www.emf.muohio.edu > |
 
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Alberto Diaspro |
EBSA2009 Last days for early registration rates!
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Dear friends,
a reminder... these are the last days for early regsitration rates at EBSA2009. An exciting program will be published on next week accordingly to the Pleanry and iNvited lecturers that you can find on the www.ebsa2009.org webpage. Genoa is always marvellous but in July is at the top! All the best Alby ---------------------------------------- Alberto Diaspro Head, Spectroscopy, Scanning Force and Nanoscopy Unit The Italian Institute of Technology -IIT Via Morego, 30 16163 - Genova (Italy) phone: +39 010 71781503 fax: +39 010 720321 http://www.iit.it [hidden email] Professor of Applied Physics Department of Physics University of Genova Via Dodecaneso, 33 16146 Genova - Italy tel. +39 010 353 6426 fax. +39 010 314218 http://www.lambs.it [hidden email] ------------------------------------------------------- |
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In reply to this post by Lauran Oomen
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, I refer back to a thread from two years ago when 37C immersion oils were discussed. We have been using the Cargille 37DF oil since then for live cell imaging. However we have just been informed by the local supplier that Cargille has discontinued DF oils. Does anyone know if there is a suitable replacement for use at 37C on a confocal. Regards, Adrian ______________________________________________ Dr Adrian Smith Manager, Cytometry & Imaging Facilities Centenary Institute http://www.centenary.org.au Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 On 23/03/2009, at 11:58 PM, [hidden email] wrote: > Dear all, > > As someone who is so directly involved (being the first author of the publication mentioned below), at first I resisted reacting to this thread. Now I would like to mention that information about the 37DF immersion oil can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) and secondly, that those of you who might be interested in a pdf of our publication on this oil can write directly to me. > > Please note: NO commercial interest. > > Lauran > -------------------------------------------------------------- > Lauran Oomen > Manager Digital Microscopy Facility (H003) > NKI-AVL > Plesmanlaan 121 > PO Box 90203 > 1006 BE Amsterdam > The Netherlands > > phone +31 205126080 > [hidden email] > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier > Sent: maandag 23 maart 2009 11:42 > To: [hidden email] > Subject: Re: Immersion Oil - 37oC > > I would like to point out that Cargille has a new oil for fluorescence > imaging at 37°C, called 37DF, that has very low auto fluorescence. It > is not on their website, but I could get the product pdf sent to me by > a Cargille person, so I can send it if someone is interested. I'm > trying to get some here in France, but none of the french Cargille > distributors has heard about it yet... It has been found to be quite > good for fluorescence imaging of live cells at 37°C, according to this > paper in J Microscopy : > > Immersion oil for high-resolution live-cell imaging at 37 degrees C: > optical and physical characteristics. > Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. > J Microsc. 2008 Nov;232(2):353-61. > > > On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> wrote: >> >> Hi all, >> >> >> >> Using immersion oil at 37oC. >> >> >> >> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, 250 and 500 ml, unfortunately all filled with the same 518F oil. According to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little extra information is offered on the Zeiss website. >> >> >> >> The only info I can gleam from the web [other than the ubiquitous 518F safety data Sheet] is the following >> >> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, n(e)=1.518 (23°C), halogen-free) >> >> >> >> So not much comment about 37oC use. PeCon's site [who supply most of Zeiss's decent incubator stuff] have no opinion on the matter either. >> >> >> >> The 518F oil safety sheet mentions "Use of the Substance / Preparation: For application information, consult the processing instructions. Processing instructions or technical >> >> information sheet available on request.". I'll ask our knowledgeable local Zeiss Confocal rep for more details, as we are just installing a Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead confocal. >> >> Cargilee's site in comparison is passionate about all things immersion oil: >> http://www.cargille.com/immeroil.shtml >> http://www.cargille.com/immeroilselection.shtml >> and their excellent, if elderly, 'primer' >> http://www.cargille.com/immersionoilmicroscope.shtml >> >> From postings to this server back in 2006, and Cargille's site not mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 probably autofluoresces, so it's likely better for live cell phase-contrast [halogen lamp] transmission microscopy [but then often so are dry objectives]. I'll stick with standard 518F immersion oil at 37oC though, it's always imaged well enough on Zeiss microscopes at that temperature [and at least at 37oC it never became cloudy due to crystal precipitation]. Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick ruthlessly with the same brand on each microscope. A lot of our live cell work is/was done with low power [20x Phase] air objectives though. >> >> I do notice that immersion oil often ingresses into oil immersion objective internal optics with time, although strangely it rarely notices when imaging [like dirt on much of the internal optics, you must mostly focus through it]. One microscopy core manager even commented to me that he considers oil objectives 'consumables'. The cost of 'repair' [often the same price as a new objective] is so high we frequently have to live with it [I advise users that our say 40x objective image quality is suspect for this reason]. In truth we don't notice any drop in image quality when it must have happened, although you can see it 'smeared' on the optics when looking through the removed objective, using a magnifying glass, bright room light and a 'cleaned' objective top lens. >> >> Old listerver posting on Cargille 37 attached below. >> >> Regards >> >> Keith >> >> PS missed this link on the last post: >> >> Dissolving crystals in Zeiss 518F oil >> http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf >> >> --------------------------------------------------------------------------- >> Dr Keith J. Morris, >> Molecular Cytogenetics and Microscopy Core, >> Laboratory 00/069 and 00/070, >> The Wellcome Trust Centre for Human Genetics, >> Roosevelt Drive, >> Oxford OX3 7BN, >> United Kingdom. >> >> Telephone: +44 (0)1865 287568 >> Email: [hidden email] >> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >> >> >> >> Subject: >> >> Re: Fluorescence from Type 37 Immersion Oil >> >> From: >> >> Vitaly Boyko <[hidden email]> >> >> Reply-To: >> >> Vitaly Boyko <[hidden email]> >> >> Date: >> >> Wed, 16 Aug 2006 10:23:20 -0400 >> >> Content-Type: >> >> text/plain >> >> >> >> Search the CONFOCAL archive at >> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> >> >> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) with >> >> the Nikon type A or Cargille type DF. >> >> >> >> >> >> >> >> Hi Jason, >> >> >> >> I wonder why you have tested the Cargille FF imm. oil, as the refractive >> >> index of FF oil is too far off for the standard glass (1.518) ?????!!!!! >> >> >> >> Actually, I haven't noticed the "very bad" autofluorescence of the Cargille >> >> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The difference is >> >> less than 10%. >> >> >> >> The problem with the Nikon oil is its dispenser and/or viscosity - it is >> >> often annoying "fighting" with the air bubbles. >> >> >> >> What is about the Invitrogen/Mol.Probes mounting media autofluorescence? >> >> >> >> I am fed up with the irreproducibility of the background autofluorescence >> >> using the ProlongGold mounting media!!! That is a serious problem compared >> >> to the issue of the immersion oils. >> >> >> >> Cheers, >> >> >> >> Vitaly >> >> >> >> NCI-Frederick >> >> >> >> >> >> ----- Original Message ----- >> >> From: "Kilgore, Jason" <[hidden email]> >> >> To: <[hidden email]> >> >> Sent: Tuesday, August 15, 2006 7:26 PM >> >> Subject: Re: Fluorescence from Type 37 Immersion Oil >> >> >> >> >> >>> Search the CONFOCAL archive at >> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >>> >> >>> Back in 2000 I performed a comparison of four different immersion oils >> >>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for >> >>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter >> >>> sets) and general resolution at 60x on a Nikon E400. I didn't test >> >>> Cargille 37. >> >>> >> >>> Green autofluorescence tended to be worst for all four, but was least >> >>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) >> >>> Cargille DF. However, the resolution was unacceptable for Cargille FF. >> >>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to >> >>> very low (acridine orange), with the same comparative trend applying. >> >>> >> >>> Thus, ever since we have been using Nikon Type A with no complaints for >> >>> most of our imaging. >> >>> >> >>> Feel free to contact me personally and I can send you the data. >> >>> >> >>> Jason >> >>> >> >>> Jason A. Kilgore >> >>> Cell Biology / Histology >> >>> Molecular Probes/Invitrogen >> >>> [hidden email] >> >>> >> >>> >> >>> -----Original Message----- >> >>> From: Confocal Microscopy List [mailto:[hidden email]] On >> >>> Behalf Of S. Brunet >> >>> Sent: Tuesday, August 15, 2006 11:19 AM >> >>> To: [hidden email] >> >>> Subject: Fluorescence from Type 37 Immersion Oil >> >>> >> >>> Search the CONFOCAL archive at >> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >>> >> >>> Hello to all, >> >>> >> >>> We recently began using the Cargille 37 oil with the temperature chamber >> >>> on the >> >>> microscope. I found significant fluorescence from the oil when exposed >> >>> to the >> >>> HBO lamp (I could not see the eYFP because of the blue background). >> >>> >> >>> So I used the 20x air objective and I was able to obtain an image from >> >>> the >> >>> fluorescence from a drop of 37 oil placed on a coverslip as a sample >> >>> (excitation at 488 or 458nm and pinhole at the usual confocal setting). >> >>> >> >>> Is this the norm? >> >>> >> >>> Regards, >> >>> Sophie >> >>> _______________________________________ >> >>> Sophie M.K. Brunet, Ph.D. >> >>> Research Officer >> >>> Optical Spectroscopist, Laser Systems and Applications >> >>> [hidden email] >> >>> 306-966-1719 (office) 306-966-1702 (fax) >> >>> _______________________________________ >> >>> Saskatchewan Structural Sciences Centre >> >>> University of Saskatchewan >> >>> Thorvaldson Bldg. >> >>> 110 Science Place >> >>> Saskatoon, SK S7N 5C9 >> >>> >> >> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Adrian, just curious, did they give a reason for discontinuing these types of oils? John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > > I refer back to a thread from two years ago when 37C immersion oils were discussed. > > We have been using the Cargille 37DF oil since then for live cell imaging. > > However we have just been informed by the local supplier that Cargille has discontinued DF oils. > > Does anyone know if there is a suitable replacement for use at 37C on a confocal. > > Regards, > > Adrian > > ______________________________________________ > Dr Adrian Smith > Manager, Cytometry & Imaging Facilities > Centenary Institute > http://www.centenary.org.au > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > >> Dear all, >> >> As someone who is so directly involved (being the first author of the publication mentioned below), at first I resisted reacting to this thread. Now I would like to mention that information about the 37DF immersion oil can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) and secondly, that those of you who might be interested in a pdf of our publication on this oil can write directly to me. >> >> Please note: NO commercial interest. >> >> Lauran >> -------------------------------------------------------------- >> Lauran Oomen >> Manager Digital Microscopy Facility (H003) >> NKI-AVL >> Plesmanlaan 121 >> PO Box 90203 >> 1006 BE Amsterdam >> The Netherlands >> >> phone +31 205126080 >> [hidden email] >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier >> Sent: maandag 23 maart 2009 11:42 >> To: [hidden email] >> Subject: Re: Immersion Oil - 37oC >> >> I would like to point out that Cargille has a new oil for fluorescence >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It >> is not on their website, but I could get the product pdf sent to me by >> a Cargille person, so I can send it if someone is interested. I'm >> trying to get some here in France, but none of the french Cargille >> distributors has heard about it yet... It has been found to be quite >> good for fluorescence imaging of live cells at 37°C, according to this >> paper in J Microscopy : >> >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: >> optical and physical characteristics. >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. >> J Microsc. 2008 Nov;232(2):353-61. >> >> >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> wrote: >>> >>> Hi all, >>> >>> >>> >>> Using immersion oil at 37oC. >>> >>> >>> >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, 250 and 500 ml, unfortunately all filled with the same 518F oil. According to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little extra information is offered on the Zeiss website. >>> >>> >>> >>> The only info I can gleam from the web [other than the ubiquitous 518F safety data Sheet] is the following >>> >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, n(e)=1.518 (23°C), halogen-free) >>> >>> >>> >>> So not much comment about 37oC use. PeCon's site [who supply most of Zeiss's decent incubator stuff] have no opinion on the matter either. >>> >>> >>> >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: For application information, consult the processing instructions. Processing instructions or technical >>> >>> information sheet available on request.". I'll ask our knowledgeable local Zeiss Confocal rep for more details, as we are just installing a Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead confocal. >>> >>> Cargilee's site in comparison is passionate about all things immersion oil: >>> http://www.cargille.com/immeroil.shtml >>> http://www.cargille.com/immeroilselection.shtml >>> and their excellent, if elderly, 'primer' >>> http://www.cargille.com/immersionoilmicroscope.shtml >>> >>> From postings to this server back in 2006, and Cargille's site not mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 probably autofluoresces, so it's likely better for live cell phase-contrast [halogen lamp] transmission microscopy [but then often so are dry objectives]. I'll stick with standard 518F immersion oil at 37oC though, it's always imaged well enough on Zeiss microscopes at that temperature [and at least at 37oC it never became cloudy due to crystal precipitation]. Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick ruthlessly with the same brand on each microscope. A lot of our live cell work is/was done with low power [20x Phase] air objectives though. >>> >>> I do notice that immersion oil often ingresses into oil immersion objective internal optics with time, although strangely it rarely notices when imaging [like dirt on much of the internal optics, you must mostly focus through it]. One microscopy core manager even commented to me that he considers oil objectives 'consumables'. The cost of 'repair' [often the same price as a new objective] is so high we frequently have to live with it [I advise users that our say 40x objective image quality is suspect for this reason]. In truth we don't notice any drop in image quality when it must have happened, although you can see it 'smeared' on the optics when looking through the removed objective, using a magnifying glass, bright room light and a 'cleaned' objective top lens. >>> >>> Old listerver posting on Cargille 37 attached below. >>> >>> Regards >>> >>> Keith >>> >>> PS missed this link on the last post: >>> >>> Dissolving crystals in Zeiss 518F oil >>> http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf >>> >>> --------------------------------------------------------------------------- >>> Dr Keith J. Morris, >>> Molecular Cytogenetics and Microscopy Core, >>> Laboratory 00/069 and 00/070, >>> The Wellcome Trust Centre for Human Genetics, >>> Roosevelt Drive, >>> Oxford OX3 7BN, >>> United Kingdom. >>> >>> Telephone: +44 (0)1865 287568 >>> Email: [hidden email] >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >>> >>> >>> >>> Subject: >>> >>> Re: Fluorescence from Type 37 Immersion Oil >>> >>> From: >>> >>> Vitaly Boyko <[hidden email]> >>> >>> Reply-To: >>> >>> Vitaly Boyko <[hidden email]> >>> >>> Date: >>> >>> Wed, 16 Aug 2006 10:23:20 -0400 >>> >>> Content-Type: >>> >>> text/plain >>> >>> >>> >>> Search the CONFOCAL archive at >>> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>> >>> >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) with >>> >>> the Nikon type A or Cargille type DF. >>> >>> >>> >>> >>> >>> >>> >>> Hi Jason, >>> >>> >>> >>> I wonder why you have tested the Cargille FF imm. oil, as the refractive >>> >>> index of FF oil is too far off for the standard glass (1.518) ?????!!!!! >>> >>> >>> >>> Actually, I haven't noticed the "very bad" autofluorescence of the Cargille >>> >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The difference is >>> >>> less than 10%. >>> >>> >>> >>> The problem with the Nikon oil is its dispenser and/or viscosity - it is >>> >>> often annoying "fighting" with the air bubbles. >>> >>> >>> >>> What is about the Invitrogen/Mol.Probes mounting media autofluorescence? >>> >>> >>> >>> I am fed up with the irreproducibility of the background autofluorescence >>> >>> using the ProlongGold mounting media!!! That is a serious problem compared >>> >>> to the issue of the immersion oils. >>> >>> >>> >>> Cheers, >>> >>> >>> >>> Vitaly >>> >>> >>> >>> NCI-Frederick >>> >>> >>> >>> >>> >>> ----- Original Message ----- >>> >>> From: "Kilgore, Jason" <[hidden email]> >>> >>> To: <[hidden email]> >>> >>> Sent: Tuesday, August 15, 2006 7:26 PM >>> >>> Subject: Re: Fluorescence from Type 37 Immersion Oil >>> >>> >>> >>> >>> >>>> Search the CONFOCAL archive at >>> >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>>> >>> >>>> Back in 2000 I performed a comparison of four different immersion oils >>> >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for >>> >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter >>> >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test >>> >>>> Cargille 37. >>> >>>> >>> >>>> Green autofluorescence tended to be worst for all four, but was least >>> >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) >>> >>>> Cargille DF. However, the resolution was unacceptable for Cargille FF. >>> >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to >>> >>>> very low (acridine orange), with the same comparative trend applying. >>> >>>> >>> >>>> Thus, ever since we have been using Nikon Type A with no complaints for >>> >>>> most of our imaging. >>> >>>> >>> >>>> Feel free to contact me personally and I can send you the data. >>> >>>> >>> >>>> Jason >>> >>>> >>> >>>> Jason A. Kilgore >>> >>>> Cell Biology / Histology >>> >>>> Molecular Probes/Invitrogen >>> >>>> [hidden email] >>> >>>> >>> >>>> >>> >>>> -----Original Message----- >>> >>>> From: Confocal Microscopy List [mailto:[hidden email]] On >>> >>>> Behalf Of S. Brunet >>> >>>> Sent: Tuesday, August 15, 2006 11:19 AM >>> >>>> To: [hidden email] >>> >>>> Subject: Fluorescence from Type 37 Immersion Oil >>> >>>> >>> >>>> Search the CONFOCAL archive at >>> >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>>> >>> >>>> Hello to all, >>> >>>> >>> >>>> We recently began using the Cargille 37 oil with the temperature chamber >>> >>>> on the >>> >>>> microscope. I found significant fluorescence from the oil when exposed >>> >>>> to the >>> >>>> HBO lamp (I could not see the eYFP because of the blue background). >>> >>>> >>> >>>> So I used the 20x air objective and I was able to obtain an image from >>> >>>> the >>> >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample >>> >>>> (excitation at 488 or 458nm and pinhole at the usual confocal setting). >>> >>>> >>> >>>> Is this the norm? >>> >>>> >>> >>>> Regards, >>> >>>> Sophie >>> >>>> _______________________________________ >>> >>>> Sophie M.K. Brunet, Ph.D. >>> >>>> Research Officer >>> >>>> Optical Spectroscopist, Laser Systems and Applications >>> >>>> [hidden email] >>> >>>> 306-966-1719 (office) 306-966-1702 (fax) >>> >>>> _______________________________________ >>> >>>> Saskatchewan Structural Sciences Centre >>> >>>> University of Saskatchewan >>> >>>> Thorvaldson Bldg. >>> >>>> 110 Science Place >>> >>>> Saskatoon, SK S7N 5C9 >>> >>>> >>> >>> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Also, is it the same as 'type 37'? I use 'Type 37' myself but I hadn't heard they were discontinuing it! Craig On Mon, Mar 21, 2011 at 4:32 PM, John Oreopoulos < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Adrian, just curious, did they give a reason for discontinuing these types > of oils? > > John Oreopoulos > Research Assistant > Spectral Applied Research > Richmond Hill, Ontario > Canada > > > On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi all, > > > > I refer back to a thread from two years ago when 37C immersion oils were > discussed. > > > > We have been using the Cargille 37DF oil since then for live cell > imaging. > > > > However we have just been informed by the local supplier that Cargille > has discontinued DF oils. > > > > Does anyone know if there is a suitable replacement for use at 37C on a > confocal. > > > > Regards, > > > > Adrian > > > > ______________________________________________ > > Dr Adrian Smith > > Manager, Cytometry & Imaging Facilities > > Centenary Institute > > http://www.centenary.org.au > > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > > > >> Dear all, > >> > >> As someone who is so directly involved (being the first author of the > publication mentioned below), at first I resisted reacting to this thread. > Now I would like to mention that information about the 37DF immersion oil > can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) > and secondly, that those of you who might be interested in a pdf of our > publication on this oil can write directly to me. > >> > >> Please note: NO commercial interest. > >> > >> Lauran > >> -------------------------------------------------------------- > >> Lauran Oomen > >> Manager Digital Microscopy Facility (H003) > >> NKI-AVL > >> Plesmanlaan 121 > >> PO Box 90203 > >> 1006 BE Amsterdam > >> The Netherlands > >> > >> phone +31 205126080 > >> [hidden email] > >> > >> -----Original Message----- > >> From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Christophe Leterrier > >> Sent: maandag 23 maart 2009 11:42 > >> To: [hidden email] > >> Subject: Re: Immersion Oil - 37oC > >> > >> I would like to point out that Cargille has a new oil for fluorescence > >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It > >> is not on their website, but I could get the product pdf sent to me by > >> a Cargille person, so I can send it if someone is interested. I'm > >> trying to get some here in France, but none of the french Cargille > >> distributors has heard about it yet... It has been found to be quite > >> good for fluorescence imaging of live cells at 37°C, according to this > >> paper in J Microscopy : > >> > >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: > >> optical and physical characteristics. > >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. > >> J Microsc. 2008 Nov;232(2):353-61. > >> > >> > >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> > wrote: > >>> > >>> Hi all, > >>> > >>> > >>> > >>> Using immersion oil at 37oC. > >>> > >>> > >>> > >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, > 250 and 500 ml, unfortunately all filled with the same 518F oil. According > to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little > extra information is offered on the Zeiss website. > >>> > >>> > >>> > >>> The only info I can gleam from the web [other than the ubiquitous 518F > safety data Sheet] is the following > >>> > >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, > n(e)=1.518 (23°C), halogen-free) > >>> > >>> > >>> > >>> So not much comment about 37oC use. PeCon's site [who supply most of > Zeiss's decent incubator stuff] have no opinion on the matter either. > >>> > >>> > >>> > >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: > For application information, consult the processing instructions. Processing > instructions or technical > >>> > >>> information sheet available on request.". I'll ask our knowledgeable > local Zeiss Confocal rep for more details, as we are just installing a > Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead > confocal. > >>> > >>> Cargilee's site in comparison is passionate about all things immersion > oil: > >>> http://www.cargille.com/immeroil.shtml > >>> http://www.cargille.com/immeroilselection.shtml > >>> and their excellent, if elderly, 'primer' > >>> http://www.cargille.com/immersionoilmicroscope.shtml > >>> > >>> From postings to this server back in 2006, and Cargille's site not > mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 > probably autofluoresces, so it's likely better for live cell phase-contrast > [halogen lamp] transmission microscopy [but then often so are dry > objectives]. I'll stick with standard 518F immersion oil at 37oC though, > it's always imaged well enough on Zeiss microscopes at that temperature [and > at least at 37oC it never became cloudy due to crystal precipitation]. > Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick > ruthlessly with the same brand on each microscope. A lot of our live cell > work is/was done with low power [20x Phase] air objectives though. > >>> > >>> I do notice that immersion oil often ingresses into oil immersion > objective internal optics with time, although strangely it rarely notices > when imaging [like dirt on much of the internal optics, you must mostly > focus through it]. One microscopy core manager even commented to me that he > considers oil objectives 'consumables'. The cost of 'repair' [often the same > price as a new objective] is so high we frequently have to live with it [I > advise users that our say 40x objective image quality is suspect for this > reason]. In truth we don't notice any drop in image quality when it must > have happened, although you can see it 'smeared' on the optics when looking > through the removed objective, using a magnifying glass, bright room light > and a 'cleaned' objective top lens. > >>> > >>> Old listerver posting on Cargille 37 attached below. > >>> > >>> Regards > >>> > >>> Keith > >>> > >>> PS missed this link on the last post: > >>> > >>> Dissolving crystals in Zeiss 518F oil > >>> > http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf > >>> > >>> > --------------------------------------------------------------------------- > >>> Dr Keith J. Morris, > >>> Molecular Cytogenetics and Microscopy Core, > >>> Laboratory 00/069 and 00/070, > >>> The Wellcome Trust Centre for Human Genetics, > >>> Roosevelt Drive, > >>> Oxford OX3 7BN, > >>> United Kingdom. > >>> > >>> Telephone: +44 (0)1865 287568 > >>> Email: [hidden email] > >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ > >>> > >>> > >>> > >>> Subject: > >>> > >>> Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> From: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Reply-To: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Date: > >>> > >>> Wed, 16 Aug 2006 10:23:20 -0400 > >>> > >>> Content-Type: > >>> > >>> text/plain > >>> > >>> > >>> > >>> Search the CONFOCAL archive at > >>> > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>> > >>> > >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) > with > >>> > >>> the Nikon type A or Cargille type DF. > >>> > >>> > >>> > >>> > >>> > >>> > >>> > >>> Hi Jason, > >>> > >>> > >>> > >>> I wonder why you have tested the Cargille FF imm. oil, as the > refractive > >>> > >>> index of FF oil is too far off for the standard glass (1.518) > ?????!!!!! > >>> > >>> > >>> > >>> Actually, I haven't noticed the "very bad" autofluorescence of the > Cargille > >>> > >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The > difference is > >>> > >>> less than 10%. > >>> > >>> > >>> > >>> The problem with the Nikon oil is its dispenser and/or viscosity - it > is > >>> > >>> often annoying "fighting" with the air bubbles. > >>> > >>> > >>> > >>> What is about the Invitrogen/Mol.Probes mounting media > autofluorescence? > >>> > >>> > >>> > >>> I am fed up with the irreproducibility of the background > autofluorescence > >>> > >>> using the ProlongGold mounting media!!! That is a serious problem > compared > >>> > >>> to the issue of the immersion oils. > >>> > >>> > >>> > >>> Cheers, > >>> > >>> > >>> > >>> Vitaly > >>> > >>> > >>> > >>> NCI-Frederick > >>> > >>> > >>> > >>> > >>> > >>> ----- Original Message ----- > >>> > >>> From: "Kilgore, Jason" <[hidden email]> > >>> > >>> To: <[hidden email]> > >>> > >>> Sent: Tuesday, August 15, 2006 7:26 PM > >>> > >>> Subject: Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> > >>> > >>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Back in 2000 I performed a comparison of four different immersion oils > >>> > >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for > >>> > >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter > >>> > >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test > >>> > >>>> Cargille 37. > >>> > >>>> > >>> > >>>> Green autofluorescence tended to be worst for all four, but was least > >>> > >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) > >>> > >>>> Cargille DF. However, the resolution was unacceptable for Cargille > FF. > >>> > >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to > >>> > >>>> very low (acridine orange), with the same comparative trend applying. > >>> > >>>> > >>> > >>>> Thus, ever since we have been using Nikon Type A with no complaints > for > >>> > >>>> most of our imaging. > >>> > >>>> > >>> > >>>> Feel free to contact me personally and I can send you the data. > >>> > >>>> > >>> > >>>> Jason > >>> > >>>> > >>> > >>>> Jason A. Kilgore > >>> > >>>> Cell Biology / Histology > >>> > >>>> Molecular Probes/Invitrogen > >>> > >>>> [hidden email] > >>> > >>>> > >>> > >>>> > >>> > >>>> -----Original Message----- > >>> > >>>> From: Confocal Microscopy List [mailto:[hidden email]] > On > >>> > >>>> Behalf Of S. Brunet > >>> > >>>> Sent: Tuesday, August 15, 2006 11:19 AM > >>> > >>>> To: [hidden email] > >>> > >>>> Subject: Fluorescence from Type 37 Immersion Oil > >>> > >>>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Hello to all, > >>> > >>>> > >>> > >>>> We recently began using the Cargille 37 oil with the temperature > chamber > >>> > >>>> on the > >>> > >>>> microscope. I found significant fluorescence from the oil when > exposed > >>> > >>>> to the > >>> > >>>> HBO lamp (I could not see the eYFP because of the blue background). > >>> > >>>> > >>> > >>>> So I used the 20x air objective and I was able to obtain an image from > >>> > >>>> the > >>> > >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample > >>> > >>>> (excitation at 488 or 458nm and pinhole at the usual confocal > setting). > >>> > >>>> > >>> > >>>> Is this the norm? > >>> > >>>> > >>> > >>>> Regards, > >>> > >>>> Sophie > >>> > >>>> _______________________________________ > >>> > >>>> Sophie M.K. Brunet, Ph.D. > >>> > >>>> Research Officer > >>> > >>>> Optical Spectroscopist, Laser Systems and Applications > >>> > >>>> [hidden email] > >>> > >>>> 306-966-1719 (office) 306-966-1702 (fax) > >>> > >>>> _______________________________________ > >>> > >>>> Saskatchewan Structural Sciences Centre > >>> > >>>> University of Saskatchewan > >>> > >>>> Thorvaldson Bldg. > >>> > >>>> 110 Science Place > >>> > >>>> Saskatoon, SK S7N 5C9 > >>> > >>>> > >>> > >>> > |
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In reply to this post by John Oreopoulos
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I spoke with one of the Cargille engineers a few weeks ago about this. He said one of the ingredients they use for the type DF oil is no longer available. They are currently reformulating the oil and hope to have it ready soon. I asked to be put on a notification list so we can order it ASAP. Dave Dave McDonald Scientific Imaging Lab Fred Hutchinson Cancer Research Center 1100 Fairview Avenue North, DE-512 Seattle, WA 98109 206-667-4205 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Oreopoulos Sent: Monday, March 21, 2011 3:33 PM To: [hidden email] Subject: Re: Immersion Oil - 37oC ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Adrian, just curious, did they give a reason for discontinuing these types of oils? John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > > I refer back to a thread from two years ago when 37C immersion oils were discussed. > > We have been using the Cargille 37DF oil since then for live cell imaging. > > However we have just been informed by the local supplier that Cargille has discontinued DF oils. > > Does anyone know if there is a suitable replacement for use at 37C on a confocal. > > Regards, > > Adrian > > ______________________________________________ > Dr Adrian Smith > Manager, Cytometry & Imaging Facilities > Centenary Institute > http://www.centenary.org.au > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > >> Dear all, >> >> As someone who is so directly involved (being the first author of the publication mentioned below), at first I resisted reacting to this thread. Now I would like to mention that information about the 37DF immersion oil can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) and secondly, that those of you who might be interested in a pdf of our publication on this oil can write directly to me. >> >> Please note: NO commercial interest. >> >> Lauran >> -------------------------------------------------------------- >> Lauran Oomen >> Manager Digital Microscopy Facility (H003) >> NKI-AVL >> Plesmanlaan 121 >> PO Box 90203 >> 1006 BE Amsterdam >> The Netherlands >> >> phone +31 205126080 >> [hidden email] >> >> -----Original Message----- >> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier >> Sent: maandag 23 maart 2009 11:42 >> To: [hidden email] >> Subject: Re: Immersion Oil - 37oC >> >> I would like to point out that Cargille has a new oil for fluorescence >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It >> is not on their website, but I could get the product pdf sent to me by >> a Cargille person, so I can send it if someone is interested. I'm >> trying to get some here in France, but none of the french Cargille >> distributors has heard about it yet... It has been found to be quite >> good for fluorescence imaging of live cells at 37°C, according to this >> paper in J Microscopy : >> >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: >> optical and physical characteristics. >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. >> J Microsc. 2008 Nov;232(2):353-61. >> >> >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> wrote: >>> >>> Hi all, >>> >>> >>> >>> Using immersion oil at 37oC. >>> >>> >>> >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, 250 and 500 ml, unfortunately all filled with the same 518F oil. According to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little extra information is offered on the Zeiss website. >>> >>> >>> >>> The only info I can gleam from the web [other than the ubiquitous 518F safety data Sheet] is the following >>> >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, n(e)=1.518 (23°C), halogen-free) >>> >>> >>> >>> So not much comment about 37oC use. PeCon's site [who supply most of Zeiss's decent incubator stuff] have no opinion on the matter either. >>> >>> >>> >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: For application information, consult the processing instructions. Processing instructions or technical >>> >>> information sheet available on request.". I'll ask our knowledgeable local Zeiss Confocal rep for more details, as we are just installing a Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead confocal. >>> >>> Cargilee's site in comparison is passionate about all things immersion oil: >>> http://www.cargille.com/immeroil.shtml >>> http://www.cargille.com/immeroilselection.shtml >>> and their excellent, if elderly, 'primer' >>> http://www.cargille.com/immersionoilmicroscope.shtml >>> >>> From postings to this server back in 2006, and Cargille's site not mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 probably autofluoresces, so it's likely better for live cell phase-contrast [halogen lamp] transmission microscopy [but then often so are dry objectives]. I'll stick with standard 518F immersion oil at 37oC though, it's always imaged well enough on Zeiss microscopes at that temperature [and at least at 37oC it never became cloudy due to crystal precipitation]. Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick ruthlessly with the same brand on each microscope. A lot of our live cell work is/was done with low power [20x Phase] air objectives though. >>> >>> I do notice that immersion oil often ingresses into oil immersion objective internal optics with time, although strangely it rarely notices when imaging [like dirt on much of the internal optics, you must mostly focus through it]. One microscopy core manager even commented to me that he considers oil objectives 'consumables'. The cost of 'repair' [often the same price as a new objective] is so high we frequently have to live with it [I advise users that our say 40x objective image quality is suspect for this reason]. In truth we don't notice any drop in image quality when it must have happened, although you can see it 'smeared' on the optics when looking through the removed objective, using a magnifying glass, bright room light and a 'cleaned' objective top lens. >>> >>> Old listerver posting on Cargille 37 attached below. >>> >>> Regards >>> >>> Keith >>> >>> PS missed this link on the last post: >>> >>> Dissolving crystals in Zeiss 518F oil >>> http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf >>> >>> --------------------------------------------------------------------------- >>> Dr Keith J. Morris, >>> Molecular Cytogenetics and Microscopy Core, >>> Laboratory 00/069 and 00/070, >>> The Wellcome Trust Centre for Human Genetics, >>> Roosevelt Drive, >>> Oxford OX3 7BN, >>> United Kingdom. >>> >>> Telephone: +44 (0)1865 287568 >>> Email: [hidden email] >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ >>> >>> >>> >>> Subject: >>> >>> Re: Fluorescence from Type 37 Immersion Oil >>> >>> From: >>> >>> Vitaly Boyko <[hidden email]> >>> >>> Reply-To: >>> >>> Vitaly Boyko <[hidden email]> >>> >>> Date: >>> >>> Wed, 16 Aug 2006 10:23:20 -0400 >>> >>> Content-Type: >>> >>> text/plain >>> >>> >>> >>> Search the CONFOCAL archive at >>> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>> >>> >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) with >>> >>> the Nikon type A or Cargille type DF. >>> >>> >>> >>> >>> >>> >>> >>> Hi Jason, >>> >>> >>> >>> I wonder why you have tested the Cargille FF imm. oil, as the refractive >>> >>> index of FF oil is too far off for the standard glass (1.518) ?????!!!!! >>> >>> >>> >>> Actually, I haven't noticed the "very bad" autofluorescence of the Cargille >>> >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The difference is >>> >>> less than 10%. >>> >>> >>> >>> The problem with the Nikon oil is its dispenser and/or viscosity - it is >>> >>> often annoying "fighting" with the air bubbles. >>> >>> >>> >>> What is about the Invitrogen/Mol.Probes mounting media autofluorescence? >>> >>> >>> >>> I am fed up with the irreproducibility of the background autofluorescence >>> >>> using the ProlongGold mounting media!!! That is a serious problem compared >>> >>> to the issue of the immersion oils. >>> >>> >>> >>> Cheers, >>> >>> >>> >>> Vitaly >>> >>> >>> >>> NCI-Frederick >>> >>> >>> >>> >>> >>> ----- Original Message ----- >>> >>> From: "Kilgore, Jason" <[hidden email]> >>> >>> To: <[hidden email]> >>> >>> Sent: Tuesday, August 15, 2006 7:26 PM >>> >>> Subject: Re: Fluorescence from Type 37 Immersion Oil >>> >>> >>> >>> >>> >>>> Search the CONFOCAL archive at >>> >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>>> >>> >>>> Back in 2000 I performed a comparison of four different immersion oils >>> >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for >>> >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter >>> >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test >>> >>>> Cargille 37. >>> >>>> >>> >>>> Green autofluorescence tended to be worst for all four, but was least >>> >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) >>> >>>> Cargille DF. However, the resolution was unacceptable for Cargille FF. >>> >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to >>> >>>> very low (acridine orange), with the same comparative trend applying. >>> >>>> >>> >>>> Thus, ever since we have been using Nikon Type A with no complaints for >>> >>>> most of our imaging. >>> >>>> >>> >>>> Feel free to contact me personally and I can send you the data. >>> >>>> >>> >>>> Jason >>> >>>> >>> >>>> Jason A. Kilgore >>> >>>> Cell Biology / Histology >>> >>>> Molecular Probes/Invitrogen >>> >>>> [hidden email] >>> >>>> >>> >>>> >>> >>>> -----Original Message----- >>> >>>> From: Confocal Microscopy List [mailto:[hidden email]] On >>> >>>> Behalf Of S. Brunet >>> >>>> Sent: Tuesday, August 15, 2006 11:19 AM >>> >>>> To: [hidden email] >>> >>>> Subject: Fluorescence from Type 37 Immersion Oil >>> >>>> >>> >>>> Search the CONFOCAL archive at >>> >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>> >>>> >>> >>>> Hello to all, >>> >>>> >>> >>>> We recently began using the Cargille 37 oil with the temperature chamber >>> >>>> on the >>> >>>> microscope. I found significant fluorescence from the oil when exposed >>> >>>> to the >>> >>>> HBO lamp (I could not see the eYFP because of the blue background). >>> >>>> >>> >>>> So I used the 20x air objective and I was able to obtain an image from >>> >>>> the >>> >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample >>> >>>> (excitation at 488 or 458nm and pinhole at the usual confocal setting). >>> >>>> >>> >>>> Is this the norm? >>> >>>> >>> >>>> Regards, >>> >>>> Sophie >>> >>>> _______________________________________ >>> >>>> Sophie M.K. Brunet, Ph.D. >>> >>>> Research Officer >>> >>>> Optical Spectroscopist, Laser Systems and Applications >>> >>>> [hidden email] >>> >>>> 306-966-1719 (office) 306-966-1702 (fax) >>> >>>> _______________________________________ >>> >>>> Saskatchewan Structural Sciences Centre >>> >>>> University of Saskatchewan >>> >>>> Thorvaldson Bldg. >>> >>>> 110 Science Place >>> >>>> Saskatoon, SK S7N 5C9 >>> >>>> >>> >>> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** When they let you know about the replacement, would you mind posting it on the list? I'm glad to see they're not just dropping the product but are taking the time to reformulate it. Thanks, Craig On Tue, Mar 22, 2011 at 10:33 AM, McDonald, David L <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I spoke with one of the Cargille engineers a few weeks ago about this. He > said one of the ingredients they use for the type DF oil is no longer > available. They are currently reformulating the oil and hope to have it > ready soon. I asked to be put on a notification list so we can order it > ASAP. > > Dave > > > Dave McDonald > Scientific Imaging Lab > Fred Hutchinson Cancer Research Center > 1100 Fairview Avenue North, DE-512 > Seattle, WA 98109 > 206-667-4205 > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of John Oreopoulos > Sent: Monday, March 21, 2011 3:33 PM > To: [hidden email] > Subject: Re: Immersion Oil - 37oC > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Adrian, just curious, did they give a reason for discontinuing these types > of oils? > > John Oreopoulos > Research Assistant > Spectral Applied Research > Richmond Hill, Ontario > Canada > > > On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi all, > > > > I refer back to a thread from two years ago when 37C immersion oils were > discussed. > > > > We have been using the Cargille 37DF oil since then for live cell > imaging. > > > > However we have just been informed by the local supplier that Cargille > has discontinued DF oils. > > > > Does anyone know if there is a suitable replacement for use at 37C on a > confocal. > > > > Regards, > > > > Adrian > > > > ______________________________________________ > > Dr Adrian Smith > > Manager, Cytometry & Imaging Facilities > > Centenary Institute > > http://www.centenary.org.au > > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > > > >> Dear all, > >> > >> As someone who is so directly involved (being the first author of the > publication mentioned below), at first I resisted reacting to this thread. > Now I would like to mention that information about the 37DF immersion oil > can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) > and secondly, that those of you who might be interested in a pdf of our > publication on this oil can write directly to me. > >> > >> Please note: NO commercial interest. > >> > >> Lauran > >> -------------------------------------------------------------- > >> Lauran Oomen > >> Manager Digital Microscopy Facility (H003) > >> NKI-AVL > >> Plesmanlaan 121 > >> PO Box 90203 > >> 1006 BE Amsterdam > >> The Netherlands > >> > >> phone +31 205126080 > >> [hidden email] > >> > >> -----Original Message----- > >> From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Christophe Leterrier > >> Sent: maandag 23 maart 2009 11:42 > >> To: [hidden email] > >> Subject: Re: Immersion Oil - 37oC > >> > >> I would like to point out that Cargille has a new oil for fluorescence > >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It > >> is not on their website, but I could get the product pdf sent to me by > >> a Cargille person, so I can send it if someone is interested. I'm > >> trying to get some here in France, but none of the french Cargille > >> distributors has heard about it yet... It has been found to be quite > >> good for fluorescence imaging of live cells at 37°C, according to this > >> paper in J Microscopy : > >> > >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: > >> optical and physical characteristics. > >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. > >> J Microsc. 2008 Nov;232(2):353-61. > >> > >> > >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> > wrote: > >>> > >>> Hi all, > >>> > >>> > >>> > >>> Using immersion oil at 37oC. > >>> > >>> > >>> > >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, > 250 and 500 ml, unfortunately all filled with the same 518F oil. According > to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little > extra information is offered on the Zeiss website. > >>> > >>> > >>> > >>> The only info I can gleam from the web [other than the ubiquitous 518F > safety data Sheet] is the following > >>> > >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, > n(e)=1.518 (23°C), halogen-free) > >>> > >>> > >>> > >>> So not much comment about 37oC use. PeCon's site [who supply most of > Zeiss's decent incubator stuff] have no opinion on the matter either. > >>> > >>> > >>> > >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: > For application information, consult the processing instructions. Processing > instructions or technical > >>> > >>> information sheet available on request.". I'll ask our knowledgeable > local Zeiss Confocal rep for more details, as we are just installing a > Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead > confocal. > >>> > >>> Cargilee's site in comparison is passionate about all things immersion > oil: > >>> http://www.cargille.com/immeroil.shtml > >>> http://www.cargille.com/immeroilselection.shtml > >>> and their excellent, if elderly, 'primer' > >>> http://www.cargille.com/immersionoilmicroscope.shtml > >>> > >>> From postings to this server back in 2006, and Cargille's site not > mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 > probably autofluoresces, so it's likely better for live cell phase-contrast > [halogen lamp] transmission microscopy [but then often so are dry > objectives]. I'll stick with standard 518F immersion oil at 37oC though, > it's always imaged well enough on Zeiss microscopes at that temperature [and > at least at 37oC it never became cloudy due to crystal precipitation]. > Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick > ruthlessly with the same brand on each microscope. A lot of our live cell > work is/was done with low power [20x Phase] air objectives though. > >>> > >>> I do notice that immersion oil often ingresses into oil immersion > objective internal optics with time, although strangely it rarely notices > when imaging [like dirt on much of the internal optics, you must mostly > focus through it]. One microscopy core manager even commented to me that he > considers oil objectives 'consumables'. The cost of 'repair' [often the same > price as a new objective] is so high we frequently have to live with it [I > advise users that our say 40x objective image quality is suspect for this > reason]. In truth we don't notice any drop in image quality when it must > have happened, although you can see it 'smeared' on the optics when looking > through the removed objective, using a magnifying glass, bright room light > and a 'cleaned' objective top lens. > >>> > >>> Old listerver posting on Cargille 37 attached below. > >>> > >>> Regards > >>> > >>> Keith > >>> > >>> PS missed this link on the last post: > >>> > >>> Dissolving crystals in Zeiss 518F oil > >>> > http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf > >>> > >>> > --------------------------------------------------------------------------- > >>> Dr Keith J. Morris, > >>> Molecular Cytogenetics and Microscopy Core, > >>> Laboratory 00/069 and 00/070, > >>> The Wellcome Trust Centre for Human Genetics, > >>> Roosevelt Drive, > >>> Oxford OX3 7BN, > >>> United Kingdom. > >>> > >>> Telephone: +44 (0)1865 287568 > >>> Email: [hidden email] > >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ > >>> > >>> > >>> > >>> Subject: > >>> > >>> Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> From: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Reply-To: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Date: > >>> > >>> Wed, 16 Aug 2006 10:23:20 -0400 > >>> > >>> Content-Type: > >>> > >>> text/plain > >>> > >>> > >>> > >>> Search the CONFOCAL archive at > >>> > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>> > >>> > >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) > with > >>> > >>> the Nikon type A or Cargille type DF. > >>> > >>> > >>> > >>> > >>> > >>> > >>> > >>> Hi Jason, > >>> > >>> > >>> > >>> I wonder why you have tested the Cargille FF imm. oil, as the > refractive > >>> > >>> index of FF oil is too far off for the standard glass (1.518) > ?????!!!!! > >>> > >>> > >>> > >>> Actually, I haven't noticed the "very bad" autofluorescence of the > Cargille > >>> > >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The > difference is > >>> > >>> less than 10%. > >>> > >>> > >>> > >>> The problem with the Nikon oil is its dispenser and/or viscosity - it > is > >>> > >>> often annoying "fighting" with the air bubbles. > >>> > >>> > >>> > >>> What is about the Invitrogen/Mol.Probes mounting media > autofluorescence? > >>> > >>> > >>> > >>> I am fed up with the irreproducibility of the background > autofluorescence > >>> > >>> using the ProlongGold mounting media!!! That is a serious problem > compared > >>> > >>> to the issue of the immersion oils. > >>> > >>> > >>> > >>> Cheers, > >>> > >>> > >>> > >>> Vitaly > >>> > >>> > >>> > >>> NCI-Frederick > >>> > >>> > >>> > >>> > >>> > >>> ----- Original Message ----- > >>> > >>> From: "Kilgore, Jason" <[hidden email]> > >>> > >>> To: <[hidden email]> > >>> > >>> Sent: Tuesday, August 15, 2006 7:26 PM > >>> > >>> Subject: Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> > >>> > >>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Back in 2000 I performed a comparison of four different immersion oils > >>> > >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for > >>> > >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter > >>> > >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test > >>> > >>>> Cargille 37. > >>> > >>>> > >>> > >>>> Green autofluorescence tended to be worst for all four, but was least > >>> > >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) > >>> > >>>> Cargille DF. However, the resolution was unacceptable for Cargille > FF. > >>> > >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to > >>> > >>>> very low (acridine orange), with the same comparative trend applying. > >>> > >>>> > >>> > >>>> Thus, ever since we have been using Nikon Type A with no complaints > for > >>> > >>>> most of our imaging. > >>> > >>>> > >>> > >>>> Feel free to contact me personally and I can send you the data. > >>> > >>>> > >>> > >>>> Jason > >>> > >>>> > >>> > >>>> Jason A. Kilgore > >>> > >>>> Cell Biology / Histology > >>> > >>>> Molecular Probes/Invitrogen > >>> > >>>> [hidden email] > >>> > >>>> > >>> > >>>> > >>> > >>>> -----Original Message----- > >>> > >>>> From: Confocal Microscopy List [mailto:[hidden email]] > On > >>> > >>>> Behalf Of S. Brunet > >>> > >>>> Sent: Tuesday, August 15, 2006 11:19 AM > >>> > >>>> To: [hidden email] > >>> > >>>> Subject: Fluorescence from Type 37 Immersion Oil > >>> > >>>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Hello to all, > >>> > >>>> > >>> > >>>> We recently began using the Cargille 37 oil with the temperature > chamber > >>> > >>>> on the > >>> > >>>> microscope. I found significant fluorescence from the oil when > exposed > >>> > >>>> to the > >>> > >>>> HBO lamp (I could not see the eYFP because of the blue background). > >>> > >>>> > >>> > >>>> So I used the 20x air objective and I was able to obtain an image from > >>> > >>>> the > >>> > >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample > >>> > >>>> (excitation at 488 or 458nm and pinhole at the usual confocal > setting). > >>> > >>>> > >>> > >>>> Is this the norm? > >>> > >>>> > >>> > >>>> Regards, > >>> > >>>> Sophie > >>> > >>>> _______________________________________ > >>> > >>>> Sophie M.K. Brunet, Ph.D. > >>> > >>>> Research Officer > >>> > >>>> Optical Spectroscopist, Laser Systems and Applications > >>> > >>>> [hidden email] > >>> > >>>> 306-966-1719 (office) 306-966-1702 (fax) > >>> > >>>> _______________________________________ > >>> > >>>> Saskatchewan Structural Sciences Centre > >>> > >>>> University of Saskatchewan > >>> > >>>> Thorvaldson Bldg. > >>> > >>>> 110 Science Place > >>> > >>>> Saskatoon, SK S7N 5C9 > >>> > >>>> > >>> > >>> > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Sure Craig, will do... Dave -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Tuesday, March 22, 2011 9:42 AM To: [hidden email] Subject: Re: Immersion Oil - 37oC ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** When they let you know about the replacement, would you mind posting it on the list? I'm glad to see they're not just dropping the product but are taking the time to reformulate it. Thanks, Craig On Tue, Mar 22, 2011 at 10:33 AM, McDonald, David L <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I spoke with one of the Cargille engineers a few weeks ago about this. He > said one of the ingredients they use for the type DF oil is no longer > available. They are currently reformulating the oil and hope to have it > ready soon. I asked to be put on a notification list so we can order it > ASAP. > > Dave > > > Dave McDonald > Scientific Imaging Lab > Fred Hutchinson Cancer Research Center > 1100 Fairview Avenue North, DE-512 > Seattle, WA 98109 > 206-667-4205 > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of John Oreopoulos > Sent: Monday, March 21, 2011 3:33 PM > To: [hidden email] > Subject: Re: Immersion Oil - 37oC > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Adrian, just curious, did they give a reason for discontinuing these types > of oils? > > John Oreopoulos > Research Assistant > Spectral Applied Research > Richmond Hill, Ontario > Canada > > > On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi all, > > > > I refer back to a thread from two years ago when 37C immersion oils were > discussed. > > > > We have been using the Cargille 37DF oil since then for live cell > imaging. > > > > However we have just been informed by the local supplier that Cargille > has discontinued DF oils. > > > > Does anyone know if there is a suitable replacement for use at 37C on a > confocal. > > > > Regards, > > > > Adrian > > > > ______________________________________________ > > Dr Adrian Smith > > Manager, Cytometry & Imaging Facilities > > Centenary Institute > > http://www.centenary.org.au > > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > > > >> Dear all, > >> > >> As someone who is so directly involved (being the first author of the > publication mentioned below), at first I resisted reacting to this thread. > Now I would like to mention that information about the 37DF immersion oil > can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) > and secondly, that those of you who might be interested in a pdf of our > publication on this oil can write directly to me. > >> > >> Please note: NO commercial interest. > >> > >> Lauran > >> -------------------------------------------------------------- > >> Lauran Oomen > >> Manager Digital Microscopy Facility (H003) > >> NKI-AVL > >> Plesmanlaan 121 > >> PO Box 90203 > >> 1006 BE Amsterdam > >> The Netherlands > >> > >> phone +31 205126080 > >> [hidden email] > >> > >> -----Original Message----- > >> From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Christophe Leterrier > >> Sent: maandag 23 maart 2009 11:42 > >> To: [hidden email] > >> Subject: Re: Immersion Oil - 37oC > >> > >> I would like to point out that Cargille has a new oil for fluorescence > >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It > >> is not on their website, but I could get the product pdf sent to me by > >> a Cargille person, so I can send it if someone is interested. I'm > >> trying to get some here in France, but none of the french Cargille > >> distributors has heard about it yet... It has been found to be quite > >> good for fluorescence imaging of live cells at 37°C, according to this > >> paper in J Microscopy : > >> > >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: > >> optical and physical characteristics. > >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. > >> J Microsc. 2008 Nov;232(2):353-61. > >> > >> > >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> > wrote: > >>> > >>> Hi all, > >>> > >>> > >>> > >>> Using immersion oil at 37oC. > >>> > >>> > >>> > >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, > 250 and 500 ml, unfortunately all filled with the same 518F oil. According > to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little > extra information is offered on the Zeiss website. > >>> > >>> > >>> > >>> The only info I can gleam from the web [other than the ubiquitous 518F > safety data Sheet] is the following > >>> > >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, > n(e)=1.518 (23°C), halogen-free) > >>> > >>> > >>> > >>> So not much comment about 37oC use. PeCon's site [who supply most of > Zeiss's decent incubator stuff] have no opinion on the matter either. > >>> > >>> > >>> > >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: > For application information, consult the processing instructions. Processing > instructions or technical > >>> > >>> information sheet available on request.". I'll ask our knowledgeable > local Zeiss Confocal rep for more details, as we are just installing a > Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead > confocal. > >>> > >>> Cargilee's site in comparison is passionate about all things immersion > oil: > >>> http://www.cargille.com/immeroil.shtml > >>> http://www.cargille.com/immeroilselection.shtml > >>> and their excellent, if elderly, 'primer' > >>> http://www.cargille.com/immersionoilmicroscope.shtml > >>> > >>> From postings to this server back in 2006, and Cargille's site not > mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 > probably autofluoresces, so it's likely better for live cell phase-contrast > [halogen lamp] transmission microscopy [but then often so are dry > objectives]. I'll stick with standard 518F immersion oil at 37oC though, > it's always imaged well enough on Zeiss microscopes at that temperature [and > at least at 37oC it never became cloudy due to crystal precipitation]. > Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick > ruthlessly with the same brand on each microscope. A lot of our live cell > work is/was done with low power [20x Phase] air objectives though. > >>> > >>> I do notice that immersion oil often ingresses into oil immersion > objective internal optics with time, although strangely it rarely notices > when imaging [like dirt on much of the internal optics, you must mostly > focus through it]. One microscopy core manager even commented to me that he > considers oil objectives 'consumables'. The cost of 'repair' [often the same > price as a new objective] is so high we frequently have to live with it [I > advise users that our say 40x objective image quality is suspect for this > reason]. In truth we don't notice any drop in image quality when it must > have happened, although you can see it 'smeared' on the optics when looking > through the removed objective, using a magnifying glass, bright room light > and a 'cleaned' objective top lens. > >>> > >>> Old listerver posting on Cargille 37 attached below. > >>> > >>> Regards > >>> > >>> Keith > >>> > >>> PS missed this link on the last post: > >>> > >>> Dissolving crystals in Zeiss 518F oil > >>> > http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf > >>> > >>> > --------------------------------------------------------------------------- > >>> Dr Keith J. Morris, > >>> Molecular Cytogenetics and Microscopy Core, > >>> Laboratory 00/069 and 00/070, > >>> The Wellcome Trust Centre for Human Genetics, > >>> Roosevelt Drive, > >>> Oxford OX3 7BN, > >>> United Kingdom. > >>> > >>> Telephone: +44 (0)1865 287568 > >>> Email: [hidden email] > >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ > >>> > >>> > >>> > >>> Subject: > >>> > >>> Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> From: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Reply-To: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Date: > >>> > >>> Wed, 16 Aug 2006 10:23:20 -0400 > >>> > >>> Content-Type: > >>> > >>> text/plain > >>> > >>> > >>> > >>> Search the CONFOCAL archive at > >>> > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>> > >>> > >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) > with > >>> > >>> the Nikon type A or Cargille type DF. > >>> > >>> > >>> > >>> > >>> > >>> > >>> > >>> Hi Jason, > >>> > >>> > >>> > >>> I wonder why you have tested the Cargille FF imm. oil, as the > refractive > >>> > >>> index of FF oil is too far off for the standard glass (1.518) > ?????!!!!! > >>> > >>> > >>> > >>> Actually, I haven't noticed the "very bad" autofluorescence of the > Cargille > >>> > >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The > difference is > >>> > >>> less than 10%. > >>> > >>> > >>> > >>> The problem with the Nikon oil is its dispenser and/or viscosity - it > is > >>> > >>> often annoying "fighting" with the air bubbles. > >>> > >>> > >>> > >>> What is about the Invitrogen/Mol.Probes mounting media > autofluorescence? > >>> > >>> > >>> > >>> I am fed up with the irreproducibility of the background > autofluorescence > >>> > >>> using the ProlongGold mounting media!!! That is a serious problem > compared > >>> > >>> to the issue of the immersion oils. > >>> > >>> > >>> > >>> Cheers, > >>> > >>> > >>> > >>> Vitaly > >>> > >>> > >>> > >>> NCI-Frederick > >>> > >>> > >>> > >>> > >>> > >>> ----- Original Message ----- > >>> > >>> From: "Kilgore, Jason" <[hidden email]> > >>> > >>> To: <[hidden email]> > >>> > >>> Sent: Tuesday, August 15, 2006 7:26 PM > >>> > >>> Subject: Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> > >>> > >>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Back in 2000 I performed a comparison of four different immersion oils > >>> > >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for > >>> > >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter > >>> > >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test > >>> > >>>> Cargille 37. > >>> > >>>> > >>> > >>>> Green autofluorescence tended to be worst for all four, but was least > >>> > >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) > >>> > >>>> Cargille DF. However, the resolution was unacceptable for Cargille > FF. > >>> > >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to > >>> > >>>> very low (acridine orange), with the same comparative trend applying. > >>> > >>>> > >>> > >>>> Thus, ever since we have been using Nikon Type A with no complaints > for > >>> > >>>> most of our imaging. > >>> > >>>> > >>> > >>>> Feel free to contact me personally and I can send you the data. > >>> > >>>> > >>> > >>>> Jason > >>> > >>>> > >>> > >>>> Jason A. Kilgore > >>> > >>>> Cell Biology / Histology > >>> > >>>> Molecular Probes/Invitrogen > >>> > >>>> [hidden email] > >>> > >>>> > >>> > >>>> > >>> > >>>> -----Original Message----- > >>> > >>>> From: Confocal Microscopy List [mailto:[hidden email]] > On > >>> > >>>> Behalf Of S. Brunet > >>> > >>>> Sent: Tuesday, August 15, 2006 11:19 AM > >>> > >>>> To: [hidden email] > >>> > >>>> Subject: Fluorescence from Type 37 Immersion Oil > >>> > >>>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Hello to all, > >>> > >>>> > >>> > >>>> We recently began using the Cargille 37 oil with the temperature > chamber > >>> > >>>> on the > >>> > >>>> microscope. I found significant fluorescence from the oil when > exposed > >>> > >>>> to the > >>> > >>>> HBO lamp (I could not see the eYFP because of the blue background). > >>> > >>>> > >>> > >>>> So I used the 20x air objective and I was able to obtain an image from > >>> > >>>> the > >>> > >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample > >>> > >>>> (excitation at 488 or 458nm and pinhole at the usual confocal > setting). > >>> > >>>> > >>> > >>>> Is this the norm? > >>> > >>>> > >>> > >>>> Regards, > >>> > >>>> Sophie > >>> > >>>> _______________________________________ > >>> > >>>> Sophie M.K. Brunet, Ph.D. > >>> > >>>> Research Officer > >>> > >>>> Optical Spectroscopist, Laser Systems and Applications > >>> > >>>> [hidden email] > >>> > >>>> 306-966-1719 (office) 306-966-1702 (fax) > >>> > >>>> _______________________________________ > >>> > >>>> Saskatchewan Structural Sciences Centre > >>> > >>>> University of Saskatchewan > >>> > >>>> Thorvaldson Bldg. > >>> > >>>> 110 Science Place > >>> > >>>> Saskatoon, SK S7N 5C9 > >>> > >>>> > >>> > >>> > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The Cargille new formulation, called Type LDF, is now available for evaluation....received my sample today. I don't believe it can be ordered yet but you can contact them for a free evaluation sample. The one spec they mentioned to me that was different from the old Type DF is the viscosity index which is 500 vs. 330 for DF. Dave -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Tuesday, March 22, 2011 9:42 AM To: [hidden email] Subject: Re: Immersion Oil - 37oC ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** When they let you know about the replacement, would you mind posting it on the list? I'm glad to see they're not just dropping the product but are taking the time to reformulate it. Thanks, Craig On Tue, Mar 22, 2011 at 10:33 AM, McDonald, David L <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I spoke with one of the Cargille engineers a few weeks ago about this. He > said one of the ingredients they use for the type DF oil is no longer > available. They are currently reformulating the oil and hope to have it > ready soon. I asked to be put on a notification list so we can order it > ASAP. > > Dave > > > Dave McDonald > Scientific Imaging Lab > Fred Hutchinson Cancer Research Center > 1100 Fairview Avenue North, DE-512 > Seattle, WA 98109 > 206-667-4205 > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of John Oreopoulos > Sent: Monday, March 21, 2011 3:33 PM > To: [hidden email] > Subject: Re: Immersion Oil - 37oC > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Adrian, just curious, did they give a reason for discontinuing these types > of oils? > > John Oreopoulos > Research Assistant > Spectral Applied Research > Richmond Hill, Ontario > Canada > > > On 2011-03-21, at 6:28 PM, Adrian Smith wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi all, > > > > I refer back to a thread from two years ago when 37C immersion oils were > discussed. > > > > We have been using the Cargille 37DF oil since then for live cell > imaging. > > > > However we have just been informed by the local supplier that Cargille > has discontinued DF oils. > > > > Does anyone know if there is a suitable replacement for use at 37C on a > confocal. > > > > Regards, > > > > Adrian > > > > ______________________________________________ > > Dr Adrian Smith > > Manager, Cytometry & Imaging Facilities > > Centenary Institute > > http://www.centenary.org.au > > Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. > > Ph: 61-2-9565-6189 Fax: 61-2-9565-6101 > > > > On 23/03/2009, at 11:58 PM, [hidden email] wrote: > > > >> Dear all, > >> > >> As someone who is so directly involved (being the first author of the > publication mentioned below), at first I resisted reacting to this thread. > Now I would like to mention that information about the 37DF immersion oil > can be found on Cargille's website (http://www.cargille.com/immeroil.shtml) > and secondly, that those of you who might be interested in a pdf of our > publication on this oil can write directly to me. > >> > >> Please note: NO commercial interest. > >> > >> Lauran > >> -------------------------------------------------------------- > >> Lauran Oomen > >> Manager Digital Microscopy Facility (H003) > >> NKI-AVL > >> Plesmanlaan 121 > >> PO Box 90203 > >> 1006 BE Amsterdam > >> The Netherlands > >> > >> phone +31 205126080 > >> [hidden email] > >> > >> -----Original Message----- > >> From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Christophe Leterrier > >> Sent: maandag 23 maart 2009 11:42 > >> To: [hidden email] > >> Subject: Re: Immersion Oil - 37oC > >> > >> I would like to point out that Cargille has a new oil for fluorescence > >> imaging at 37°C, called 37DF, that has very low auto fluorescence. It > >> is not on their website, but I could get the product pdf sent to me by > >> a Cargille person, so I can send it if someone is interested. I'm > >> trying to get some here in France, but none of the french Cargille > >> distributors has heard about it yet... It has been found to be quite > >> good for fluorescence imaging of live cells at 37°C, according to this > >> paper in J Microscopy : > >> > >> Immersion oil for high-resolution live-cell imaging at 37 degrees C: > >> optical and physical characteristics. > >> Oomen LC, Sacher R, Brocks HH, Zwier JM, Brakenhoff GJ, Jalink K. > >> J Microsc. 2008 Nov;232(2):353-61. > >> > >> > >> On Mon, Mar 23, 2009 at 11:18, Keith Morris <[hidden email]> > wrote: > >>> > >>> Hi all, > >>> > >>> > >>> > >>> Using immersion oil at 37oC. > >>> > >>> > >>> > >>> Zeiss sell a range of low fluorescence immersion oils: bottles of 20, > 250 and 500 ml, unfortunately all filled with the same 518F oil. According > to Zeiss it's apparently 'compatible' with our Zeiss microscopes, but little > extra information is offered on the Zeiss website. > >>> > >>> > >>> > >>> The only info I can gleam from the web [other than the ubiquitous 518F > safety data Sheet] is the following > >>> > >>> Immersion oil "Immersol" 518 F fluorescence free, (ISO 8036-1/2, > n(e)=1.518 (23°C), halogen-free) > >>> > >>> > >>> > >>> So not much comment about 37oC use. PeCon's site [who supply most of > Zeiss's decent incubator stuff] have no opinion on the matter either. > >>> > >>> > >>> > >>> The 518F oil safety sheet mentions "Use of the Substance / Preparation: > For application information, consult the processing instructions. Processing > instructions or technical > >>> > >>> information sheet available on request.". I'll ask our knowledgeable > local Zeiss Confocal rep for more details, as we are just installing a > Zeiss/PeCon 37oC live cell incubator here on our Zeiss 510 MetaHead > confocal. > >>> > >>> Cargilee's site in comparison is passionate about all things immersion > oil: > >>> http://www.cargille.com/immeroil.shtml > >>> http://www.cargille.com/immeroilselection.shtml > >>> and their excellent, if elderly, 'primer' > >>> http://www.cargille.com/immersionoilmicroscope.shtml > >>> > >>> From postings to this server back in 2006, and Cargille's site not > mentioning 'non-fluorescence' for type 37, it appears that Cargille 37 > probably autofluoresces, so it's likely better for live cell phase-contrast > [halogen lamp] transmission microscopy [but then often so are dry > objectives]. I'll stick with standard 518F immersion oil at 37oC though, > it's always imaged well enough on Zeiss microscopes at that temperature [and > at least at 37oC it never became cloudy due to crystal precipitation]. > Similarly the Cargille DF and FF seem fine to me at 37oC, and we stick > ruthlessly with the same brand on each microscope. A lot of our live cell > work is/was done with low power [20x Phase] air objectives though. > >>> > >>> I do notice that immersion oil often ingresses into oil immersion > objective internal optics with time, although strangely it rarely notices > when imaging [like dirt on much of the internal optics, you must mostly > focus through it]. One microscopy core manager even commented to me that he > considers oil objectives 'consumables'. The cost of 'repair' [often the same > price as a new objective] is so high we frequently have to live with it [I > advise users that our say 40x objective image quality is suspect for this > reason]. In truth we don't notice any drop in image quality when it must > have happened, although you can see it 'smeared' on the optics when looking > through the removed objective, using a magnifying glass, bright room light > and a 'cleaned' objective top lens. > >>> > >>> Old listerver posting on Cargille 37 attached below. > >>> > >>> Regards > >>> > >>> Keith > >>> > >>> PS missed this link on the last post: > >>> > >>> Dissolving crystals in Zeiss 518F oil > >>> > http://www.zeiss.com/C1256F8500454979/0/E8CD09DFA520787FC1256F86004B5FFB/$file/immersol-crystals.pdf > >>> > >>> > --------------------------------------------------------------------------- > >>> Dr Keith J. Morris, > >>> Molecular Cytogenetics and Microscopy Core, > >>> Laboratory 00/069 and 00/070, > >>> The Wellcome Trust Centre for Human Genetics, > >>> Roosevelt Drive, > >>> Oxford OX3 7BN, > >>> United Kingdom. > >>> > >>> Telephone: +44 (0)1865 287568 > >>> Email: [hidden email] > >>> Web-pages: http://www.well.ox.ac.uk/cytogenetics/ > >>> > >>> > >>> > >>> Subject: > >>> > >>> Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> From: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Reply-To: > >>> > >>> Vitaly Boyko <[hidden email]> > >>> > >>> Date: > >>> > >>> Wed, 16 Aug 2006 10:23:20 -0400 > >>> > >>> Content-Type: > >>> > >>> text/plain > >>> > >>> > >>> > >>> Search the CONFOCAL archive at > >>> > >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>> > >>> > >>> PS What would be more appropriate to compare is Zeiss 518F (not 518C!) > with > >>> > >>> the Nikon type A or Cargille type DF. > >>> > >>> > >>> > >>> > >>> > >>> > >>> > >>> Hi Jason, > >>> > >>> > >>> > >>> I wonder why you have tested the Cargille FF imm. oil, as the > refractive > >>> > >>> index of FF oil is too far off for the standard glass (1.518) > ?????!!!!! > >>> > >>> > >>> > >>> Actually, I haven't noticed the "very bad" autofluorescence of the > Cargille > >>> > >>> type DF versus Zeiss 518 in the CFP, YFP or FRET channels. The > difference is > >>> > >>> less than 10%. > >>> > >>> > >>> > >>> The problem with the Nikon oil is its dispenser and/or viscosity - it > is > >>> > >>> often annoying "fighting" with the air bubbles. > >>> > >>> > >>> > >>> What is about the Invitrogen/Mol.Probes mounting media > autofluorescence? > >>> > >>> > >>> > >>> I am fed up with the irreproducibility of the background > autofluorescence > >>> > >>> using the ProlongGold mounting media!!! That is a serious problem > compared > >>> > >>> to the issue of the immersion oils. > >>> > >>> > >>> > >>> Cheers, > >>> > >>> > >>> > >>> Vitaly > >>> > >>> > >>> > >>> NCI-Frederick > >>> > >>> > >>> > >>> > >>> > >>> ----- Original Message ----- > >>> > >>> From: "Kilgore, Jason" <[hidden email]> > >>> > >>> To: <[hidden email]> > >>> > >>> Sent: Tuesday, August 15, 2006 7:26 PM > >>> > >>> Subject: Re: Fluorescence from Type 37 Immersion Oil > >>> > >>> > >>> > >>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Back in 2000 I performed a comparison of four different immersion oils > >>> > >>>> (Zeiss 518C, Nikon Type A, Cargille Type DF, and Cargille Type FF) for > >>> > >>>> autofluorescence (using DAPI, FITC, acridine orange, and TRITC filter > >>> > >>>> sets) and general resolution at 60x on a Nikon E400. I didn't test > >>> > >>>> Cargille 37. > >>> > >>>> > >>> > >>>> Green autofluorescence tended to be worst for all four, but was least > >>> > >>>> for Cargille FF, followed by Nikon Type A, then Zeiss, then (very bad) > >>> > >>>> Cargille DF. However, the resolution was unacceptable for Cargille > FF. > >>> > >>>> Autofluorescence in the other channels was moderate (DAPI, TRITC) to > >>> > >>>> very low (acridine orange), with the same comparative trend applying. > >>> > >>>> > >>> > >>>> Thus, ever since we have been using Nikon Type A with no complaints > for > >>> > >>>> most of our imaging. > >>> > >>>> > >>> > >>>> Feel free to contact me personally and I can send you the data. > >>> > >>>> > >>> > >>>> Jason > >>> > >>>> > >>> > >>>> Jason A. Kilgore > >>> > >>>> Cell Biology / Histology > >>> > >>>> Molecular Probes/Invitrogen > >>> > >>>> [hidden email] > >>> > >>>> > >>> > >>>> > >>> > >>>> -----Original Message----- > >>> > >>>> From: Confocal Microscopy List [mailto:[hidden email]] > On > >>> > >>>> Behalf Of S. Brunet > >>> > >>>> Sent: Tuesday, August 15, 2006 11:19 AM > >>> > >>>> To: [hidden email] > >>> > >>>> Subject: Fluorescence from Type 37 Immersion Oil > >>> > >>>> > >>> > >>>> Search the CONFOCAL archive at > >>> > >>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >>> > >>>> > >>> > >>>> Hello to all, > >>> > >>>> > >>> > >>>> We recently began using the Cargille 37 oil with the temperature > chamber > >>> > >>>> on the > >>> > >>>> microscope. I found significant fluorescence from the oil when > exposed > >>> > >>>> to the > >>> > >>>> HBO lamp (I could not see the eYFP because of the blue background). > >>> > >>>> > >>> > >>>> So I used the 20x air objective and I was able to obtain an image from > >>> > >>>> the > >>> > >>>> fluorescence from a drop of 37 oil placed on a coverslip as a sample > >>> > >>>> (excitation at 488 or 458nm and pinhole at the usual confocal > setting). > >>> > >>>> > >>> > >>>> Is this the norm? > >>> > >>>> > >>> > >>>> Regards, > >>> > >>>> Sophie > >>> > >>>> _______________________________________ > >>> > >>>> Sophie M.K. Brunet, Ph.D. > >>> > >>>> Research Officer > >>> > >>>> Optical Spectroscopist, Laser Systems and Applications > >>> > >>>> [hidden email] > >>> > >>>> 306-966-1719 (office) 306-966-1702 (fax) > >>> > >>>> _______________________________________ > >>> > >>>> Saskatchewan Structural Sciences Centre > >>> > >>>> University of Saskatchewan > >>> > >>>> Thorvaldson Bldg. > >>> > >>>> 110 Science Place > >>> > >>>> Saskatoon, SK S7N 5C9 > >>> > >>>> > >>> > >>> > |
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