Claire-
In general the Swedish site, Human Protein Atlas, is the place to look for antibodies that work. I always find it by Googling.
They have checked many antibodies that are commercially available and they tell you on their site which are capable of localizing what appears to be the right protein.
Carol Heckman
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From: Confocal Microscopy List [
[hidden email]] On Behalf Of Claire Brown, Dr. [
[hidden email]]
Sent: Thursday, August 26, 2010 11:54 AM
To:
[hidden email]
Subject: Immunostaining of Tissue
I got this request from one of my facility users:
“We would like to localize endogenous CFTR in intracellular i.e. in ER, cis-Golgi, trans-Golgi and secretory granules in native tissue samples.
Is there a good reference that summarizes the best marker proteins and antibodies to use for such immunolocalizations?”
Does anyone know of a good reference for this?
Sincerely,
Claire
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Claire M. Brown, PhD
McGill University Imaging Facility
3649 Promenade Sir William Osler
Bellini Building, Rm137
Montreal, Quebec, H3G 0B1
514-398-4400 X00795 (Phone)
514-398-7452 (FAX)
[hidden email]
www.lifesciencescomplex.mcgill.ca/imaging
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