Input about image cell boundaries detection during image analysis

[Jean-Pierre CLAMME-2]

Hello,

I have GFP fluorescent cells and I would like to detect the cell limites based on the GFP fluorescence. Right now I just choose a treshold based on what I see or based on the ration between the fluorescence and the background.
So I was just curious to hear about your methods to choose a treshold for cell boudaries detection. I would like to somehow normalize the detection and make it a liitle bit less arbitrary.

Thank you,

JP

[Paulucci, Adriana A.]

Hi Leoncio.
I just want to double check with you if you are coming tomorrow morning at 9.
I really appreaciate if you can come at least to tell me a little bit about the stuff we have here, and also to tell me what to do with some folders left here, so I can get little bit organized.
We can start the trainnig, then, on the other week. Did Dr. Motamedi contacted you?

Sincerely,

Adriana Paulucci-Holthauzen, Ph.D.
Manager, Optical Imaging Laboratory (OIL)
University of Texas Medical Branch at Galveston
10.120 MRB
Phone: [phone]
________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Jean-Pierre CLAMME [[hidden email]]
Sent: Friday, March 27, 2009 4:10 PM
To: [hidden email]
Subject: Input about image cell boundaries detection during image analysis


Hello,

I have GFP fluorescent cells and I would like to detect the cell limites based on the GFP fluorescence. Right now I just choose a treshold based on what I see or based on the ration between the fluorescence and the background.
So I was just curious to hear about your methods to choose a treshold for cell boudaries detection. I would like to somehow normalize the detection and make it a liitle bit less arbitrary.

Thank you,

JP