Installation of dichroics for the Yokogawa CSU-10

[Stephen Bunnell]

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Hi all,

        Has anyone had any experience with installing a dichroic into the static mount of a Yokogawa CSU-10 spinning disc unit? I have three dichroic mounts and need to swap out one dichroic for an updated version. Yokogawa no longer supports the CSU-10, so any advice would be appreciated.

        Best regards,

        Steve
___

Stephen C. Bunnell, Ph.D.
Associate Professor
Tufts University Medical School
Department of Integrative
    Physiology and Pathobiology
M&V Bldg., Room 702
136 Harrison Ave.
Boston, MA 02111
___

[Alison J. North]

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Hi Steve,

We ordered and installed new dichroics through Spectral Applied (now
part of Andor) when they improved our CSU-10 system with the Borealis
upgrade and new lasers.  I believe the dichroics themselves were from
Chroma.  Hope that helps....

Best,

Alison


On 5/24/2016 1:10 PM, Bunnell, Stephen C. wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> Has anyone had any experience with installing a dichroic into the static mount of a Yokogawa CSU-10 spinning disc unit? I have three dichroic mounts and need to swap out one dichroic for an updated version. Yokogawa no longer supports the CSU-10, so any advice would be appreciated.
>
> Best regards,
>
> Steve
> ___
>
> Stephen C. Bunnell, Ph.D.
> Associate Professor
> Tufts University Medical School
> Department of Integrative
>      Physiology and Pathobiology
> M&V Bldg., Room 702
> 136 Harrison Ave.
> Boston, MA 02111
> ___

--
Alison J. North, Ph.D.,
Research Associate Professor and
Senior Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office ++ 212 327 7488
Tel: lab     ++ 212 327 7486
Fax:         ++ 212 327 7489

[George Peeters-2]

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Hi Steve:
We do it for labs when we visit, or you can try it yourself.

It can be done in about 15 minutes. The thing to be careful with is not to remove the screws completely and not to scratch the light absorbing surface of the retaining spring and light shield.
Having the right screw driver helps;  PH000 x 40 (Wiha) works. The shields are available but expensive and delicate. The newer model CSUX and W1 systems are easier.

Be careful to insert the dichroic all the way up to the seat limit and have the dichroic oriented correctly with the coated side facing the microscope. Also avoid doing this in dusty rooms and do not use canned air to blow off dust,... better done with squeeze bulb with filtered air intake.

Not rocket science, but does take some care.

Best

George


George Peeters, M.D., M.S.
Pres. Solamere Technology Group inc.
1515 Military Way
Salt Lake City, UT.  84103

Cell    801 232-6911





On May 24, 2016, at 11:10 AM, Bunnell, Stephen C. <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> Has anyone had any experience with installing a dichroic into the static mount of a Yokogawa CSU-10 spinning disc unit? I have three dichroic mounts and need to swap out one dichroic for an updated version. Yokogawa no longer supports the CSU-10, so any advice would be appreciated.
>
> Best regards,
>
> Steve
> ___
>
> Stephen C. Bunnell, Ph.D.
> Associate Professor
> Tufts University Medical School
> Department of Integrative
>    Physiology and Pathobiology
> M&V Bldg., Room 702
> 136 Harrison Ave.
> Boston, MA 02111
> ___

[John Oreopoulos]

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Hi Stephen,

For this type of job, I'm going to recommend that you have someone else who's done this before help you out. These dichroics are extremely thin and fragile, and I have seen the results when they are not installed properly or carefully - shattered glass and/or scratched disks which lead to even more expensive problems. The exchange should also be done in a clean environment to prevent dust from landing on the disks.

Please contact your local Andor representative for a quote for this kind of work, or let me know if need help locating a representative.

Cheers,

John Oreopoulos
Staff Scientist
Spectral Applied Research
A Division of Andor Technology
www.spectral.ca


On 2016-05-25, at 10:36 AM, Alison North wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Steve,
>
> We ordered and installed new dichroics through Spectral Applied (now part of Andor) when they improved our CSU-10 system with the Borealis upgrade and new lasers.  I believe the dichroics themselves were from Chroma.  Hope that helps....
>
> Best,
>
> Alison
>
>
> On 5/24/2016 1:10 PM, Bunnell, Stephen C. wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi all,
>>
>> Has anyone had any experience with installing a dichroic into the static mount of a Yokogawa CSU-10 spinning disc unit? I have three dichroic mounts and need to swap out one dichroic for an updated version. Yokogawa no longer supports the CSU-10, so any advice would be appreciated.
>>
>> Best regards,
>>
>> Steve
>> ___
>>
>> Stephen C. Bunnell, Ph.D.
>> Associate Professor
>> Tufts University Medical School
>> Department of Integrative
>>     Physiology and Pathobiology
>> M&V Bldg., Room 702
>> 136 Harrison Ave.
>> Boston, MA 02111
>> ___
>
> --
> Alison J. North, Ph.D.,
> Research Associate Professor and
> Senior Director of the Bio-Imaging Resource Center,
> The Rockefeller University,
> 1230 York Avenue,
> New York,
> NY 10065.
> Tel: office ++ 212 327 7488
> Tel: lab     ++ 212 327 7486
> Fax:         ++ 212 327 7489