Installing a Blue laser for DAPI

[ugez323]

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Dear All

I was wondering if anybody had experience of installing a blue laser for
DAPI into a BioRad Radiance 2100 system and whether this is relatively
easily done and also some rough figures for cost.

Thanks

Jonathan


Professor J.C. Knowles BSc(hons), PhD, FIMMM, CEng, FRSC, CSci
Professor of Biomaterials Science
Head of Division of Biomaterials and Tissue Engineering,
UCL Eastman Dental Institute,
University College London,
256 Gray's Inn Road,
London WC1X 8LD. UK.
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Editor, Journal of Biomaterials Applications
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[Craig Brideau]

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Depending on how 'blue' it needs to be (wavelength?) your laser can run from $10-25k (assuming you can stick with diode, or maybe argon-ion).  In terms of getting it into the microscope; if the existing lasers are already fiber-coupled then you can have a fiber splitter rigged up or just replace one of the existing lasers.  This will probably cost another couple $k to have the fiber assembly constructed, less if you can find something 'off-the-shelf'.  If they are just fired in freespace via a port then you will have to put a dichroic or the like somewhere in the system to guide in the beam from the new laser along the path of one of the existing lasers.  This again will probably cost a couple $k or more, depending on if you have to have any custom machining done to fit the parts into the existing mechanism.

Hope this helps!

Craig

On Thu, Aug 28, 2008 at 8:04 AM, ugez323 <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear All

I was wondering if anybody had experience of installing a blue laser for
DAPI into a BioRad Radiance 2100 system and whether this is relatively
easily done and also some rough figures for cost.

Thanks

Jonathan


Professor J.C. Knowles BSc(hons), PhD, FIMMM, CEng, FRSC, CSci
Professor of Biomaterials Science
Head of Division of Biomaterials and Tissue Engineering,
UCL Eastman Dental Institute,
University College London,
256 Gray's Inn Road,
London WC1X 8LD. UK.
Tel [phone]
Fax [phone]
Mobile [phone]
Skype Me
Editor, Journal of Biomaterials Applications
Email [hidden email]
Eastman website; http://www.eastman.ucl.ac.uk
Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck/
This email represents the views of the sender alone and must not be
construed as representing the views of the Eastman Dental Institute. It may
contain confidential information and may be protected by law as a legally
privileged document and copyright work. Its content should not be disclosed
and it should not be given or copied to anyone other than the person(s)
named or referenced above. If you have received this email in error, please
contact the sender.