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Intravital Imaging

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Stephen Cody

Intravital Imaging

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Intravital Imaging

Dear List,

We are setting up a new multiphoton instrument for intravital imaging. One of the proposals is to monitor the growth of tumours in mice over several months. This will require anaesthesia, surgery, imaging, and then stitching the animal back up and moving it back to an animal holding facility.

- There is no possibility of moving the instrument into the animal house.

- The mice will be immune compromised.

- Our animal house through quarantine rules will not accept the animals back.

- Our confocal instrument is in a PC2 facility

- The approach to the multiphoton dark room is via a public lift, open office space for scientists, and a very busy PC2 general laboratory.

- We have access to a quarantine animal housing facility.

We are concerned naturally for the welfare of the animal, but also exposing numerous scientists (not involved with the project) to mouse allergens.

What we propose:

- To house the mice in micro-isolators in a quarantine facility.

- Transport the mouse in a micro-isolator box via a public lift and through the laboratory to the imaging facility.

- Place the micro-isolator box in a Class II BioHazard hood to reduce allergens. And to help protect the immune deficient animal from infection.

- Anaesthetise the mouse.

- Any surgery would be done in the Class II BioHazard hood.

- This hood is located within the imaging facilities preparation laboratory, and immediately outside the door of the Multiphoton dark room.

- Image the animal on an upright microscope with a dipping lens.

- Return the animal to the Class II hood for stitches and returned to the micro-isolator box for transport back to the quarantine facility.

In addition or perhaps instead of the Class II hood we are considering an “AiroCide” photocatalytic air purifying system http://www.airocide.com/ . The AiroCide system could be placed in the multiphoton dark room itself, as an addition to the Class II hood in the preparation area.

Could anyone please comment on our proposal? Are there simpler solutions?

No commercial affiliation with AiroCide.

Thanks

Stephen H. Cody

Microscopy Manager

Central Resource for Advanced Microscopy
Ludwig Institute for Cancer Research

PO Box 2008 Royal Melbourne Hospital

Victoria, 3050

Australia

Tel: 61 3 9341 3155 Fax: 61 3 9341 3104

email: [hidden email]

www.ludwig.edu.au/labs/confocal.html

www.ludwig.edu.au/confocal

Tip: Learn how to receive reminders about you microscope booking:

www.ludwig.edu.au/confocal/Local/Booking_Hint.htm

This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research does not waiver any rights if you have received this communication in error.
The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research.

Adrian Smith-6

Re: Intravital Imaging

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Stephen,

We have a very similar SOP for moving mice between our multi-photon and IVIS imaging rooms.

We've been trialling an air-purifying unit (UV and filters) but it hasn't be tried in the imaging rooms yet.

Regards,

Adrian Smith

Centenary Institute, Sydney, Australia

On 06/06/2008, at 11:24 AM, Stephen Cody wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear List,
We are setting up a new multiphoton instrument for intravital imaging. One of the proposals is to monitor the growth of tumours in mice over several months. This will require anaesthesia, surgery, imaging, and then stitching the animal back up and moving it back to an animal holding facility.
- There is no possibility of moving the instrument into the animal house.
- The mice will be immune compromised.
- Our animal house through quarantine rules will not accept the animals back.
- Our confocal instrument is in a PC2 facility
- The approach to the multiphoton dark room is via a public lift, open office space for scientists, and a very busy PC2 general laboratory.
- We have access to a quarantine animal housing facility.
We are concerned naturally for the welfare of the animal, but also exposing numerous scientists (not involved with the project) to mouse allergens.
What we propose:
- To house the mice in micro-isolators in a quarantine facility.
- Transport the mouse in a micro-isolator box via a public lift and through the laboratory to the imaging facility.
- Place the micro-isolator box in a Class II BioHazard hood to reduce allergens. And to help protect the immune deficient animal from infection.
- Anaesthetise the mouse.
- Any surgery would be done in the Class II BioHazard hood.
- This hood is located within the imaging facilities preparation laboratory, and immediately outside the door of the Multiphoton dark room.
- Image the animal on an upright microscope with a dipping lens.
- Return the animal to the Class II hood for stitches and returned to the micro-isolator box for transport back to the quarantine facility.
In addition or perhaps instead of the Class II hood we are considering an “AiroCide” photocatalytic air purifying system http://www.airocide.com/ . The AiroCide system could be placed in the multiphoton dark room itself, as an addition to the Class II hood in the preparation area.
Could anyone please comment on our proposal? Are there simpler solutions?
No commercial affiliation with AiroCide.
Thanks
Stephen H. Cody
Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008 Royal Melbourne Hospital
Victoria, 3050
Australia
Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
email: [hidden email]
www.ludwig.edu.au/labs/confocal.html
www.ludwig.edu.au/confocal
Tip: Learn how to receive reminders about you microscope booking:
www.ludwig.edu.au/confocal/Local/Booking_Hint.htm

This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research does not waiver any rights if you have received this communication in error.
The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research.