JC-1 in real time?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear List - Has anyone tried to use JC-1 for real-time measurements? Is aggregation of the dye reversible? Mike Model |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It is, very much so. You can see it happening as you image and cells start showing distress. Anda Cornea -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Monday, January 31, 2011 3:43 PM To: [hidden email] Subject: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear List - Has anyone tried to use JC-1 for real-time measurements? Is aggregation of the dye reversible? Mike Model |
 
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Dr. Mark A. DeCoster |
Re: JC-1 in real time?- disaggregation possible?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** And so if you somehow save the cells (from stress) can disaggregation be observed or is that not possible? -most of the results I have seen show only one-way= more aggregation with more stress- Mark DeCoster Mark A. DeCoster, Ph.D. --James E. Wyche III Associate Professor in Engineering-- Biomedical Engineering and Institute for MicroManufacturing Louisiana Tech University http://www2.latech.edu/~decoster/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anda Cornea Sent: Monday, January 31, 2011 6:04 PM To: [hidden email] Subject: Re: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It is, very much so. You can see it happening as you image and cells start showing distress. Anda Cornea -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Monday, January 31, 2011 3:43 PM To: [hidden email] Subject: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear List - Has anyone tried to use JC-1 for real-time measurements? Is aggregation of the dye reversible? Mike Model |
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Anda Cornea |
Re: JC-1 in real time?- disaggregation possible?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This does not seem right to me if we are talking about aggregation of JC-1. The way I understand it is: aggregates show red fluorescence and indicate strong mitochondrial potential and little stress. Green mitochondria indicate lower potential and no aggregation. Diffuse green inside the cell indicates lack of aggregation but also release of compound from mitochondria due to loss of potential. What I see at the beginning of an experiment are nice green mitochondria with red dots, red disappears as I keep imaging the same field for a few seconds. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark A. DeCoster Sent: Monday, January 31, 2011 5:14 PM To: [hidden email] Subject: Re: JC-1 in real time?- disaggregation possible? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** And so if you somehow save the cells (from stress) can disaggregation be observed or is that not possible? -most of the results I have seen show only one-way= more aggregation with more stress- Mark DeCoster Mark A. DeCoster, Ph.D. --James E. Wyche III Associate Professor in Engineering-- Biomedical Engineering and Institute for MicroManufacturing Louisiana Tech University http://www2.latech.edu/~decoster/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anda Cornea Sent: Monday, January 31, 2011 6:04 PM To: [hidden email] Subject: Re: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It is, very much so. You can see it happening as you image and cells start showing distress. Anda Cornea -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Monday, January 31, 2011 3:43 PM To: [hidden email] Subject: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear List - Has anyone tried to use JC-1 for real-time measurements? Is aggregation of the dye reversible? Mike Model |
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Lemasters, John J. |
Re: JC-1 in real time?- disaggregation possible?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The J-aggregates of JC-1 are precipitates. When JC-1 reaches a critical concentration due to electrogenic uptake into the mitochondrial matrix, the dye precipitates. As this crystallization occurs, the JC-1 forms stacks of thousands (millions) of molecules that exhibit metachromsia due to interaction of the pi orbitals of adjacent molecules in the stacks. When mitochondria depolarize, JC-1 is released, dye concentration falls, the crystals dissolve, and metachromasia disappears. By confocal microscopy one can observe directly these precipitates. They typically fill only a portion of the matrix space and often marginate to the side, but of course it is incorrect to conclude that part of the matrix space has a different electrical potential than another part. Mr. Hoose, my high school chemistry teacher, used to refer to some of us students as insoluble residues, but for JC-1 it's not as bad as all that, and the reversible precipitation of JC-1 can sometimes be useful. John -- John J. Lemasters, MD, PhD Professor and GlaxoSmithKline Distinguished Endowed Chair Director, Center for Cell Death, Injury and Regeneration Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology Medical University of South Carolina QF213 Quadrangle Building 280 Calhoun Street, MSC 140 Charleston, SC 29425 Office: 843-792-2153 Lab: 843-792-3530 Fax: 843-792-8436 Email: [hidden email] http://academicdepartments.musc.edu/ccdir -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anda Cornea Sent: Monday, January 31, 2011 8:27 PM To: [hidden email] Subject: Re: JC-1 in real time?- disaggregation possible? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** This does not seem right to me if we are talking about aggregation of JC-1. The way I understand it is: aggregates show red fluorescence and indicate strong mitochondrial potential and little stress. Green mitochondria indicate lower potential and no aggregation. Diffuse green inside the cell indicates lack of aggregation but also release of compound from mitochondria due to loss of potential. What I see at the beginning of an experiment are nice green mitochondria with red dots, red disappears as I keep imaging the same field for a few seconds. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mark A. DeCoster Sent: Monday, January 31, 2011 5:14 PM To: [hidden email] Subject: Re: JC-1 in real time?- disaggregation possible? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** And so if you somehow save the cells (from stress) can disaggregation be observed or is that not possible? -most of the results I have seen show only one-way= more aggregation with more stress- Mark DeCoster Mark A. DeCoster, Ph.D. --James E. Wyche III Associate Professor in Engineering-- Biomedical Engineering and Institute for MicroManufacturing Louisiana Tech University http://www2.latech.edu/~decoster/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Anda Cornea Sent: Monday, January 31, 2011 6:04 PM To: [hidden email] Subject: Re: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** It is, very much so. You can see it happening as you image and cells start showing distress. Anda Cornea -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Monday, January 31, 2011 3:43 PM To: [hidden email] Subject: JC-1 in real time? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear List - Has anyone tried to use JC-1 for real-time measurements? Is aggregation of the dye reversible? Mike Model |
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