Janelia conference for imaging cores - some pointers

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Sylvie Le Guyader Sylvie Le Guyader
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Re: Janelia conference for imaging cores - some pointers

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Dear list

I fully agree that the ELMI, Eurobioimaging and all the network opportunities that exist at a country or continent level are fantastic and I strongly recommend sending facility staff for job shadowing to learn alternative ways to manage facilities.

However for staff of a small facility who feel that external input would be useful more than once per year, it can be a little difficult to know where to get practical help when a problem shows up.

Inspired by our Singaporean colleagues, we have found an easy way to start a local network: we contacted all other imaging facilities in the Stockholm/Uppsala region and suggested we should meet every second month for 2h.

All the facilities take turn in hosting the meeting. This allows everyone to be aware of the type of equipment the others have, see what they do when they want to upgrade, what type of imaging their users do...

Each host decides on the format, some like it very informal and some have it very structured with agenda and presentation. Missing a meeting is not a big deal as we often share some notes and there is another meeting soon after.

Taking turn means that there is no heavy organization for just one facility. It only takes sending a Doodle to find a date that works for most, booking a room then sending an email to confirm date and venue. And of course buying 'fika' (coffee bread) because we are in Sweden so we love our fika!! :-D

All one needs is a shared cloud storage (Dropbox style) with the shared mailing list that is updated as each facility adds new people. Our list of participants has only grown from 15 to 33 facility staff since we started, just one year ago.

We talk about very practical things, from funding to should we be listed authors on papers or not, how we deal with all the data, sharing teaching/training resources... Meeting regularly means that you know exactly who might be able to help you solve that problem that just popped up at your facility. Inviting everyone for a particularly interesting seminar is no hassle with a ready-to-use mailing list.

I personally find global/national networks very inspiring and local networks super useful! :)

Just thought I would share with you all.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: +46 (0) 73 733 5008
office: +46 (0) 8 524 811 72
LCI website



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of WHEELER Ann
Sent: den 1 november 2017 20:41
To: [hidden email]
Subject: Re: Janelia conference for imaging cores - some pointers

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Kate:


I would echo what Alison says, putting together the UKBioimaging network has been no small task. We're definitely not as well documented as our colleagues in Germany. However the UK funding network has undergone a sea-change in the past decade. Our funders are now looking for value for money in equipment grants, and seeking evidence they would benefit many projects and the resources will have an impact regionally, nationally as well as locally.


While I won't understate the amount of work a dedicated group of us (many of which were also committed ELMI attendees and EuroBioimaging-philes) put in I would say interms of continuation of funding, particularly for our more high cost imaging technologies, of it has been a well worthwhile exercise for us. Furthermore our BioImaging UK facility database, now kindly hosted by the RMS https://www.rms.org.uk/network-collaborate/facilities-database.html has been an invaluable resource. It enables us to quickly and easily identify niche technologies and specific expertise - the list has made our facility agile which has been welcomed.


I often send my staff to other cores to learn and we host visits as well. This is beneficial to all. The German BioImaging have a more formal process for this which certainly has advantages. Since European countries are relatively nearby we've also been to visit colleagues from ELMI as well.


The UK network is staffed by a group of volunteers, we have an annual meeting which is free for facility staff to attend - kindly supported by our industrial partners. We discuss matters which have been raised by others earlier on the thread. Uk Imaging facilities can be very variable - some are large and host several technologies and skilled staff scientists. Others are smaller affairs - depending on research need of the institute. Best practise in the UK would mean meeting and supporting research need and this can look different. But sharing experiences and ideas is invaluable for all.


This sounds like an interesting opportunity for the US!


Best


Ann


Dr Ann Wheeler

Head of IGMM Advanced Imaging Resource

IGMM, Edinburgh EH4 2XU, UK

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Roland Nitschke <[hidden email]>
Sent: 01 November 2017 18:34:51
To: [hidden email]
Subject: Re: Janelia conference for imaging cores - some pointers

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

The German BioImaging Network  is one of those Europian national networks mentioned by Alison.

You can find extensive information about us here:
http://www.germanbioimaging.org

We have published in 2016 an open access paper about: Advanced light microscopy core facilities: Balancing service, science and career , which covers many of the important aspects raised before by others.

You can download the paper at:
http://onlinelibrary.wiley.com/doi/10.1002/jemt.22648/abstract

I am one of the founders and coordinators of this German network and Elisa Ferrando-May is an invited speaker at the Janelia meeting.

I might also come over to the meeting if I manage to put together an attractive abstract about the local trinational imaging network
(https://www.miap.eu)  that I have set-up here in Freiburg.

Thanks Alison also to mention the ELMI meeting, which is clearly a great place for exchange beetween core facilities in Europe.

All the best

Roland





___________________________

Albert-Ludwigs-University Freiburg
Microscopy and Image Analysis Platform (MIAP) Life Imaging Center (LIC) in ZBSA Dr. Roland Nitschke
Habsburgerstr.49
D-79104 Freiburg
Germany
___________________________
E-mail: [hidden email]
phone: 49-761-2032934 or 2902
fax: 49-761-2032941
https://miap.eu/miap-units/life-imaging-center-lic/

Am 01.11.2017 um 19:12 schrieb Alison North:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Kate,
>
> I think you have raised a number of excellent suggestions here.
> Regarding your last one in particular, I feel the need to point out
> that we in the USA are WAY behind many other countries in the area of
> networking, particularly when it comes to sharing equipment.  I don't
> know whether you are familiar with the Euro-BioImaging project:
>
> http://www.eurobioimaging.eu/
>
> but this is a very interesting and laudable attempt to solve exactly
> the problem you refer to in accessing uncommon equipment. Many of the
> European countries (also, I believe, Australia) have also established
> their own country-wide networks, long before the Euro-BioImaging
> network was established.  These matters are discussed every year at
> the wonderful ELMI meeting (European Light Microscopy Initiative),
> which, in my opinion, is the most useful meeting out there for
> microscopy facility managers.  (Also jolly good fun and held in a
> fantastic location every year - thank you ELMI meeting organisers past
> and present!)  Personally, I would like to see a similar network
> established in the USA, but the sheer enormity of this task is rather
> daunting.  Maybe we could first approach it on a state or regional
> level?  This is certainly something worth discussing.
>
> All the best,
> Alison
>
>
> On 11/1/2017 2:00 PM, Kate Luby-Phelps wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi
>> -2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFaQ&c=JeTkUgVztGMmhKYjxsy2rf
>> oWYibK1YmxXez1G3oNStg&r=y3Gg5KwygGNfgoACnrKLvtQcoFinbJ43lW3fx-6okTk&m
>> =E4i8HsilKoimSALTZ1UgskIXVL1QntXVMrJIhE7Dpdc&s=FURRfkajYc71p3ruAh01np
>> 19tBddU9Xv0hQc6C7xzQI&e=
>>
>> Post images on
>> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=D
>> wIFaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=y3Gg5KwygGNfgoA
>> CnrKLvtQcoFinbJ43lW3fx-6okTk&m=E4i8HsilKoimSALTZ1UgskIXVL1QntXVMrJIhE
>> 7Dpdc&s=EQ9Y7WWaHhVJYiO1Yr5US_JJj00ThprUfqWDdkByJ48&e=
>> and include the link in your posting.
>> *****
>>
>> I had not really thought much about networking with other core
>> facilities until I saw the announcement of the conference. For me,
>> the confocal listserv essentially fills this niche as we get to pick
>> each others' brains. However, in thinking about it more, it seems to
>> me there are several ways in which a formal networking effort might
>> be helpful: 1) Since all cores large and small will share some of the
>> same challenges, perhaps identification of these areas and formation
>> of focused subnetworks or topical working groups could be useful. For
>> example, I have made some stabs at solving the need for deconvolution
>> of large volumes from slide scanners and tiled confocal z stacks but
>> have not had the time or the resources to pursue this to an endpoint.
>> If others are in the same boat, perhaps we each have different pieces
>> of the puzzle and sharing them could speed up the process of
>> eliminating what doesn't work or at least defining what the
>> roadblocks are and then having group members take on well-defined,
>> manageable tasks to try to move things forward for everyone. As
>> already mentioned, another example of a challenge we all face is
>> finding money to replace aging instrumentation and to acquire cutting
>> edge technology. Here a network for mentoring people who want to
>> apply for S10 grants could be helpful. A third possibility is a
>> network that could serve as a consortium for sharing of resources.
>> For software, this is not too difficult, as witnessed by the success
>> of ImageJ/FiJi and CellProfiler. Would it be possible to facilitate
>> access to instrumentation as well? For example, if I have an
>> occasional need for STED but do not own one and do not have a large
>> enough user group to apply for an S10, can I locate a facility that
>> has it and somehow arrange to have my sample viewed? I realize this
>> idea raises all kinds of logistical issues, including recharge costs,
>> travel costs, shipping of samples, etc., but perhaps its feasibility
>> is worth discussing.
>>
>> A conference that attempts to promote and formalize these types of
>> interactions would be worth attending.
>>
>> Kate Luby-Phelps
>> Director, Live Cell Imaging Facility and Electron Microscopy Core
>> Facility UT Southwestern Medical Center Dallas, TX
>
Kurt Anderson Kurt Anderson
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Re: Janelia conference for imaging cores - some pointers

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*****


howdy,

i think the sort of bottom-up, grass-roots approach outlined by sylvie may be the way forward and am not convinced that all networks are equally fantastic and useful. we have to evaluate what they cost in time, money, and distraction compared to the actual (not claimed) benefits they deliver.

the UK light microscopy facilities (UKLMF) meeting, which we will host this january at the francis crick institute, is a case in point. UKLMF started small just over ten years ago and has grown from the bottom up. it has helped to strengthen the UK imaging community, similar to the way that ELMI has helped build the european imaging community. both networks are largely self-funding and i think most people would agree they get more out of them than they are asked to put in. neither network aims to solve every problem we face with a committee or work package.

my suspicion is that such local (limited?) networks may provide more bang for the buck than larger, less efficient, less responsive organisations. its easier to experiment with a smaller network and figure out how to address the most pressing needs of a community, which will vary from place to place and field to field. perhaps we need to more carefully consider what things networks deliver well at different scales.

we will address this topic in a modest way at the UKLMF meeting in january, and i hope the discussion will continue at janelia and elsewhere. in a world of limited resources its crucial to make the most of what we’ve got.

happy trails,

kurt

.



On 2 Nov 2017, at 09:57, Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear list

I fully agree that the ELMI, Eurobioimaging and all the network opportunities that exist at a country or continent level are fantastic and I strongly recommend sending facility staff for job shadowing to learn alternative ways to manage facilities.

However for staff of a small facility who feel that external input would be useful more than once per year, it can be a little difficult to know where to get practical help when a problem shows up.

Inspired by our Singaporean colleagues, we have found an easy way to start a local network: we contacted all other imaging facilities in the Stockholm/Uppsala region and suggested we should meet every second month for 2h.

All the facilities take turn in hosting the meeting. This allows everyone to be aware of the type of equipment the others have, see what they do when they want to upgrade, what type of imaging their users do...

Each host decides on the format, some like it very informal and some have it very structured with agenda and presentation. Missing a meeting is not a big deal as we often share some notes and there is another meeting soon after.

Taking turn means that there is no heavy organization for just one facility. It only takes sending a Doodle to find a date that works for most, booking a room then sending an email to confirm date and venue. And of course buying 'fika' (coffee bread) because we are in Sweden so we love our fika!! :-D

All one needs is a shared cloud storage (Dropbox style) with the shared mailing list that is updated as each facility adds new people. Our list of participants has only grown from 15 to 33 facility staff since we started, just one year ago.

We talk about very practical things, from funding to should we be listed authors on papers or not, how we deal with all the data, sharing teaching/training resources... Meeting regularly means that you know exactly who might be able to help you solve that problem that just popped up at your facility. Inviting everyone for a particularly interesting seminar is no hassle with a ready-to-use mailing list.

I personally find global/national networks very inspiring and local networks super useful! :)

Just thought I would share with you all.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157 Huddinge
Sweden
mobile: +46 (0) 73 733 5008
office: +46 (0) 8 524 811 72
LCI website



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of WHEELER Ann
Sent: den 1 november 2017 20:41
To: [hidden email]<mailto:[hidden email]>
Subject: Re: Janelia conference for imaging cores - some pointers

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Kate:


I would echo what Alison says, putting together the UKBioimaging network has been no small task. We're definitely not as well documented as our colleagues in Germany. However the UK funding network has undergone a sea-change in the past decade. Our funders are now looking for value for money in equipment grants, and seeking evidence they would benefit many projects and the resources will have an impact regionally, nationally as well as locally.


While I won't understate the amount of work a dedicated group of us (many of which were also committed ELMI attendees and EuroBioimaging-philes) put in I would say interms of continuation of funding, particularly for our more high cost imaging technologies, of it has been a well worthwhile exercise for us. Furthermore our BioImaging UK facility database, now kindly hosted by the RMS https://www.rms.org.uk/network-collaborate/facilities-database.html has been an invaluable resource. It enables us to quickly and easily identify niche technologies and specific expertise - the list has made our facility agile which has been welcomed.


I often send my staff to other cores to learn and we host visits as well. This is beneficial to all. The German BioImaging have a more formal process for this which certainly has advantages. Since European countries are relatively nearby we've also been to visit colleagues from ELMI as well.


The UK network is staffed by a group of volunteers, we have an annual meeting which is free for facility staff to attend - kindly supported by our industrial partners. We discuss matters which have been raised by others earlier on the thread. Uk Imaging facilities can be very variable - some are large and host several technologies and skilled staff scientists. Others are smaller affairs - depending on research need of the institute. Best practise in the UK would mean meeting and supporting research need and this can look different. But sharing experiences and ideas is invaluable for all.


This sounds like an interesting opportunity for the US!


Best


Ann


Dr Ann Wheeler

Head of IGMM Advanced Imaging Resource

IGMM, Edinburgh EH4 2XU, UK

________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Roland Nitschke <[hidden email]>
Sent: 01 November 2017 18:34:51
To: [hidden email]
Subject: Re: Janelia conference for imaging cores - some pointers

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

The German BioImaging Network  is one of those Europian national networks mentioned by Alison.

You can find extensive information about us here:
http://www.germanbioimaging.org

We have published in 2016 an open access paper about: Advanced light microscopy core facilities: Balancing service, science and career , which covers many of the important aspects raised before by others.

You can download the paper at:
http://onlinelibrary.wiley.com/doi/10.1002/jemt.22648/abstract

I am one of the founders and coordinators of this German network and Elisa Ferrando-May is an invited speaker at the Janelia meeting.

I might also come over to the meeting if I manage to put together an attractive abstract about the local trinational imaging network
(https://www.miap.eu)  that I have set-up here in Freiburg.

Thanks Alison also to mention the ELMI meeting, which is clearly a great place for exchange beetween core facilities in Europe.

All the best

Roland





___________________________

Albert-Ludwigs-University Freiburg
Microscopy and Image Analysis Platform (MIAP) Life Imaging Center (LIC) in ZBSA Dr. Roland Nitschke
Habsburgerstr.49
D-79104 Freiburg
Germany
___________________________
E-mail: [hidden email]
phone: 49-761-2032934 or 2902
fax: 49-761-2032941
https://miap.eu/miap-units/life-imaging-center-lic/

Am 01.11.2017 um 19:12 schrieb Alison North:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Kate,

I think you have raised a number of excellent suggestions here.
Regarding your last one in particular, I feel the need to point out
that we in the USA are WAY behind many other countries in the area of
networking, particularly when it comes to sharing equipment.  I don't
know whether you are familiar with the Euro-BioImaging project:

http://www.eurobioimaging.eu/

but this is a very interesting and laudable attempt to solve exactly
the problem you refer to in accessing uncommon equipment. Many of the
European countries (also, I believe, Australia) have also established
their own country-wide networks, long before the Euro-BioImaging
network was established.  These matters are discussed every year at
the wonderful ELMI meeting (European Light Microscopy Initiative),
which, in my opinion, is the most useful meeting out there for
microscopy facility managers.  (Also jolly good fun and held in a
fantastic location every year - thank you ELMI meeting organisers past
and present!)  Personally, I would like to see a similar network
established in the USA, but the sheer enormity of this task is rather
daunting.  Maybe we could first approach it on a state or regional
level?  This is certainly something worth discussing.

All the best,
Alison


On 11/1/2017 2:00 PM, Kate Luby-Phelps wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi
-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIFaQ&c=JeTkUgVztGMmhKYjxsy2rf
oWYibK1YmxXez1G3oNStg&r=y3Gg5KwygGNfgoACnrKLvtQcoFinbJ43lW3fx-6okTk&m
=E4i8HsilKoimSALTZ1UgskIXVL1QntXVMrJIhE7Dpdc&s=FURRfkajYc71p3ruAh01np
19tBddU9Xv0hQc6C7xzQI&e=

Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=D
wIFaQ&c=JeTkUgVztGMmhKYjxsy2rfoWYibK1YmxXez1G3oNStg&r=y3Gg5KwygGNfgoA
CnrKLvtQcoFinbJ43lW3fx-6okTk&m=E4i8HsilKoimSALTZ1UgskIXVL1QntXVMrJIhE
7Dpdc&s=EQ9Y7WWaHhVJYiO1Yr5US_JJj00ThprUfqWDdkByJ48&e=
and include the link in your posting.
*****

I had not really thought much about networking with other core
facilities until I saw the announcement of the conference. For me,
the confocal listserv essentially fills this niche as we get to pick
each others' brains. However, in thinking about it more, it seems to
me there are several ways in which a formal networking effort might
be helpful: 1) Since all cores large and small will share some of the
same challenges, perhaps identification of these areas and formation
of focused subnetworks or topical working groups could be useful. For
example, I have made some stabs at solving the need for deconvolution
of large volumes from slide scanners and tiled confocal z stacks but
have not had the time or the resources to pursue this to an endpoint.
If others are in the same boat, perhaps we each have different pieces
of the puzzle and sharing them could speed up the process of
eliminating what doesn't work or at least defining what the
roadblocks are and then having group members take on well-defined,
manageable tasks to try to move things forward for everyone. As
already mentioned, another example of a challenge we all face is
finding money to replace aging instrumentation and to acquire cutting
edge technology. Here a network for mentoring people who want to
apply for S10 grants could be helpful. A third possibility is a
network that could serve as a consortium for sharing of resources.
For software, this is not too difficult, as witnessed by the success
of ImageJ/FiJi and CellProfiler. Would it be possible to facilitate
access to instrumentation as well? For example, if I have an
occasional need for STED but do not own one and do not have a large
enough user group to apply for an S10, can I locate a facility that
has it and somehow arrange to have my sample viewed? I realize this
idea raises all kinds of logistical issues, including recharge costs,
travel costs, shipping of samples, etc., but perhaps its feasibility
is worth discussing.

A conference that attempts to promote and formalize these types of
interactions would be worth attending.

Kate Luby-Phelps
Director, Live Cell Imaging Facility and Electron Microscopy Core
Facility UT Southwestern Medical Center Dallas, TX




Kurt I. Anderson
CALM Head
Crick Advanced Light Microscopy Facility
The Francis Crick Institute
1 Midland Road
London
NW1 1AT

T: +442037962949 (desk)
T: +442037960914 (lab)
M: +447894134257 (personal)
E: [hidden email]<mailto:email%[hidden email]>
W: www.crick.ac.uk<http://www.crick.ac.uk>
R: L3-2783 (office)


The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT
mcammer mcammer
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Re: Janelia conference for imaging cores - some pointers

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

It is unlikely that cores will be able to assure the funding or release time to attend networking events.

Secondly, I used to be a big fan of the Internet for hashing out issues, but now am wary of any discussions regarding administration, client issues, or vender (especially repair problems) as the people being discussed may be reading too.  For instance, the new administrative regime here would probably label the wonderful list of poster don'ts as "inappropriate" or "unprofessional" in tone.  Speech by public figures and on social media may be increasingly reckless, but most research institutions are becoming more staidly corporate and core staff are increasingly treated as guns for hire and not protected by an expectation of academic freedom.

So because 1.) of funding constraints for face-to-face meetings and 2.) loss of safety in candid speech via the web, I'm not sanguine about these calls for more sharing.


Michael Cammer, Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]    http://microscopynotes.com/ 
https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kurt Anderson
Sent: Thursday, November 02, 2017 9:05 AM
To: [hidden email]
Subject: Re: Janelia conference for imaging cores - some pointers

*****

To join, leave or search the confocal microscopy listserv, go to:

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=J0ZJkDZkbg4Dq_oHAZ2QYZAXGmMxuGDbxY-Ti45M54g&e= 

Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=APDn0cSDLkHARNzshKHf7tjCouVOwPukQG5Quhsgl64&e=  and include the link in your posting.

*****





howdy,



i think the sort of bottom-up, grass-roots approach outlined by sylvie may be the way forward and am not convinced that all networks are equally fantastic and useful. we have to evaluate what they cost in time, money, and distraction compared to the actual (not claimed) benefits they deliver.



the UK light microscopy facilities (UKLMF) meeting, which we will host this january at the francis crick institute, is a case in point. UKLMF started small just over ten years ago and has grown from the bottom up. it has helped to strengthen the UK imaging community, similar to the way that ELMI has helped build the european imaging community. both networks are largely self-funding and i think most people would agree they get more out of them than they are asked to put in. neither network aims to solve every problem we face with a committee or work package.



my suspicion is that such local (limited?) networks may provide more bang for the buck than larger, less efficient, less responsive organisations. its easier to experiment with a smaller network and figure out how to address the most pressing needs of a community, which will vary from place to place and field to field. perhaps we need to more carefully consider what things networks deliver well at different scales.



we will address this topic in a modest way at the UKLMF meeting in january, and i hope the discussion will continue at janelia and elsewhere. in a world of limited resources its crucial to make the most of what we’ve got.



happy trails,



kurt



.







On 2 Nov 2017, at 09:57, Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> wrote:



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Dear list



I fully agree that the ELMI, Eurobioimaging and all the network opportunities that exist at a country or continent level are fantastic and I strongly recommend sending facility staff for job shadowing to learn alternative ways to manage facilities.



However for staff of a small facility who feel that external input would be useful more than once per year, it can be a little difficult to know where to get practical help when a problem shows up.



Inspired by our Singaporean colleagues, we have found an easy way to start a local network: we contacted all other imaging facilities in the Stockholm/Uppsala region and suggested we should meet every second month for 2h.



All the facilities take turn in hosting the meeting. This allows everyone to be aware of the type of equipment the others have, see what they do when they want to upgrade, what type of imaging their users do...



Each host decides on the format, some like it very informal and some have it very structured with agenda and presentation. Missing a meeting is not a big deal as we often share some notes and there is another meeting soon after.



Taking turn means that there is no heavy organization for just one facility. It only takes sending a Doodle to find a date that works for most, booking a room then sending an email to confirm date and venue. And of course buying 'fika' (coffee bread) because we are in Sweden so we love our fika!! :-D



All one needs is a shared cloud storage (Dropbox style) with the shared mailing list that is updated as each facility adds new people. Our list of participants has only grown from 15 to 33 facility staff since we started, just one year ago.



We talk about very practical things, from funding to should we be listed authors on papers or not, how we deal with all the data, sharing teaching/training resources... Meeting regularly means that you know exactly who might be able to help you solve that problem that just popped up at your facility. Inviting everyone for a particularly interesting seminar is no hassle with a ready-to-use mailing list.



I personally find global/national networks very inspiring and local networks super useful! :)



Just thought I would share with you all.



Med vänlig hälsning / Best regards



Sylvie



@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD

Live Cell Imaging Facility Manager

Karolinska Institutet- Bionut Dpt

Hälsovägen 7,

Novum, G lift, floor 6

14157 Huddinge

Sweden

mobile: +46 (0) 73 733 5008

office: +46 (0) 8 524 811 72

LCI website







-----Original Message-----

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of WHEELER Ann

Sent: den 1 november 2017 20:41

To: [hidden email]<mailto:[hidden email]>

Subject: Re: Janelia conference for imaging cores - some pointers



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Kate:





I would echo what Alison says, putting together the UKBioimaging network has been no small task. We're definitely not as well documented as our colleagues in Germany. However the UK funding network has undergone a sea-change in the past decade. Our funders are now looking for value for money in equipment grants, and seeking evidence they would benefit many projects and the resources will have an impact regionally, nationally as well as locally.





While I won't understate the amount of work a dedicated group of us (many of which were also committed ELMI attendees and EuroBioimaging-philes) put in I would say interms of continuation of funding, particularly for our more high cost imaging technologies, of it has been a well worthwhile exercise for us. Furthermore our BioImaging UK facility database, now kindly hosted by the RMS https://urldefense.proofpoint.com/v2/url?u=https-3A__www.rms.org.uk_network-2Dcollaborate_facilities-2Ddatabase.html&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=SfYTdiL7cAzHO-42rgDXjvf-mFuaa65RO-IQcp5uPa4&e=  has been an invaluable resource. It enables us to quickly and easily identify niche technologies and specific expertise - the list has made our facility agile which has been welcomed.





I often send my staff to other cores to learn and we host visits as well. This is beneficial to all. The German BioImaging have a more formal process for this which certainly has advantages. Since European countries are relatively nearby we've also been to visit colleagues from ELMI as well.





The UK network is staffed by a group of volunteers, we have an annual meeting which is free for facility staff to attend - kindly supported by our industrial partners. We discuss matters which have been raised by others earlier on the thread. Uk Imaging facilities can be very variable - some are large and host several technologies and skilled staff scientists. Others are smaller affairs - depending on research need of the institute. Best practise in the UK would mean meeting and supporting research need and this can look different. But sharing experiences and ideas is invaluable for all.





This sounds like an interesting opportunity for the US!





Best





Ann





Dr Ann Wheeler



Head of IGMM Advanced Imaging Resource



IGMM, Edinburgh EH4 2XU, UK



________________________________

From: Confocal Microscopy List <[hidden email]> on behalf of Roland Nitschke <[hidden email]>

Sent: 01 November 2017 18:34:51

To: [hidden email]

Subject: Re: Janelia conference for imaging cores - some pointers



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The German BioImaging Network  is one of those Europian national networks mentioned by Alison.



You can find extensive information about us here:

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.germanbioimaging.org&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=Y0aj-wGSAoFLAqkZUZZntg0-pqFO92TeYI6l93cFeqs&e= 



We have published in 2016 an open access paper about: Advanced light microscopy core facilities: Balancing service, science and career , which covers many of the important aspects raised before by others.



You can download the paper at:

https://urldefense.proofpoint.com/v2/url?u=http-3A__onlinelibrary.wiley.com_doi_10.1002_jemt.22648_abstract&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=nRX9ododEJy8yE6KKXK873FL2qQFIl4wFxevOTpPtuQ&e= 



I am one of the founders and coordinators of this German network and Elisa Ferrando-May is an invited speaker at the Janelia meeting.



I might also come over to the meeting if I manage to put together an attractive abstract about the local trinational imaging network

(https://urldefense.proofpoint.com/v2/url?u=https-3A__www.miap.eu&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=8nD3_34aATkvbQl-w00iCX3W0fEveuNL6z_mJp5XCl8&e= )  that I have set-up here in Freiburg.



Thanks Alison also to mention the ELMI meeting, which is clearly a great place for exchange beetween core facilities in Europe.



All the best



Roland











___________________________



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Microscopy and Image Analysis Platform (MIAP) Life Imaging Center (LIC) in ZBSA Dr. Roland Nitschke

Habsburgerstr.49

D-79104 Freiburg

Germany

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Am 01.11.2017 um 19:12 schrieb Alison North:

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Dear Kate,



I think you have raised a number of excellent suggestions here.

Regarding your last one in particular, I feel the need to point out

that we in the USA are WAY behind many other countries in the area of

networking, particularly when it comes to sharing equipment.  I don't

know whether you are familiar with the Euro-BioImaging project:



https://urldefense.proofpoint.com/v2/url?u=http-3A__www.eurobioimaging.eu_&d=DQIGaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=61roJSaHo3Gu15ryZg6LiwWkzTAammqURKRsyhq-rHE&s=oCLHiEKSsM11iYQLoAnYmHXeVCEWR0Cyga_oR4qYmFY&e= 



but this is a very interesting and laudable attempt to solve exactly

the problem you refer to in accessing uncommon equipment. Many of the

European countries (also, I believe, Australia) have also established

their own country-wide networks, long before the Euro-BioImaging

network was established.  These matters are discussed every year at

the wonderful ELMI meeting (European Light Microscopy Initiative),

which, in my opinion, is the most useful meeting out there for

microscopy facility managers.  (Also jolly good fun and held in a

fantastic location every year - thank you ELMI meeting organisers past

and present!)  Personally, I would like to see a similar network

established in the USA, but the sheer enormity of this task is rather

daunting.  Maybe we could first approach it on a state or regional

level?  This is certainly something worth discussing.



All the best,

Alison





On 11/1/2017 2:00 PM, Kate Luby-Phelps wrote:

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I had not really thought much about networking with other core

facilities until I saw the announcement of the conference. For me,

the confocal listserv essentially fills this niche as we get to pick

each others' brains. However, in thinking about it more, it seems to

me there are several ways in which a formal networking effort might

be helpful: 1) Since all cores large and small will share some of the

same challenges, perhaps identification of these areas and formation

of focused subnetworks or topical working groups could be useful. For

example, I have made some stabs at solving the need for deconvolution

of large volumes from slide scanners and tiled confocal z stacks but

have not had the time or the resources to pursue this to an endpoint.

If others are in the same boat, perhaps we each have different pieces

of the puzzle and sharing them could speed up the process of

eliminating what doesn't work or at least defining what the

roadblocks are and then having group members take on well-defined,

manageable tasks to try to move things forward for everyone. As

already mentioned, another example of a challenge we all face is

finding money to replace aging instrumentation and to acquire cutting

edge technology. Here a network for mentoring people who want to

apply for S10 grants could be helpful. A third possibility is a

network that could serve as a consortium for sharing of resources.

For software, this is not too difficult, as witnessed by the success

of ImageJ/FiJi and CellProfiler. Would it be possible to facilitate

access to instrumentation as well? For example, if I have an

occasional need for STED but do not own one and do not have a large

enough user group to apply for an S10, can I locate a facility that

has it and somehow arrange to have my sample viewed? I realize this

idea raises all kinds of logistical issues, including recharge costs,

travel costs, shipping of samples, etc., but perhaps its feasibility

is worth discussing.



A conference that attempts to promote and formalize these types of

interactions would be worth attending.



Kate Luby-Phelps

Director, Live Cell Imaging Facility and Electron Microscopy Core

Facility UT Southwestern Medical Center Dallas, TX









Kurt I. Anderson

CALM Head

Crick Advanced Light Microscopy Facility

The Francis Crick Institute

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