Job Opening: Northwestern Univ Cell Imaging Facility and Nikon Imaging Center

[Teng-Leong Chew]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear colleagues,

I am pleased to announce we are looking for a microscopy
specialist to help manage the Cell Imaging Facility and
Nikon Imaging Center at Northwestern University Medical
School.

Catering to the microscopy needs of nearly 250 labs from
all three NU campuses, the rapidly expanding facility is
a scientifically stimulating place to work. Specifically
we are looking for a highly qualified individual with
good knowledge in Cell Biology, optics, software and
hardware, and at least 1-2 years of laboratory experience
in light/confocal/electron microscopy. Minimum requirement:
B.S. in biological science; Master's or Ph.D. preferred.

We offer a competitive salary and comprehensive benefits
package. For immediate consideration, send your resume
and cover to Northwestern University HR Office.

-------
You can access the Northwestern University Careers site by
going to:

http://www.northwestern.edu/hr/jobs

If you are an Internal Applicant, click on the link
"Northwestern Community Job Opportunities"

If you are a new or returning External Applicant, click on
the link "New or Returning External Applicants Job
Opportunities"

Once you are on the Careers Home page, please follow the
below steps to view the Microscopy & Imaging Spec position:
1. Click on the "Advanced Search" link located in the Basic
Job Search box.

2. Type: Microscopy & Imaging Spec  into the Keywords text
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3. Click on the Search button to view the search results.


Teng-Leong Chew, Ph.D.
Director,
Cell Imaging Facility and Nikon Imaging Center
Robert H. Lurie Comprehensive Cancer Center
Northwestern University Feinberg School of Medicine
303 E. Chicago Avenue
Chicago, IL 60611

[Liu, Hao-Yang]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

 

 

In a paper, Biophysical Journal, vol 86, p2517, 2004, the work mentioned several factors needed to be corrected when perform FRET in laser scanning confocal, by sensitized emission. One of them is to correct misfocusing deviation (page 2521). This error comes from two main sources. One is chromatic aberration, which we are quite familiar. The other is the slight differences in the collimation of the laser beams.

 

Any comments?

Are there ways to test the performance of the system?

Thanks

 

Hao-Yang Liu

[Craig Brideau]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We had a similar problem with a CARS microscope we built.  We needed two lasers to arrive at the exact same focal point and their divergences were slightly different.  We addressed the problem by installing a telescope in the path of one of the lasers.  By tweaking the focus of the telescope (just move one of the lenses in and out on the order of millimeters) you can adjust the divergence of the beam.  If you don't have any free-space access to the laser though, this could be problematic.

Craig

On Thu, May 1, 2008 at 9:44 AM, Liu, Hao-Yang <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal





In a paper, Biophysical Journal, vol 86, p2517, 2004, the work mentioned several factors needed to be corrected when perform FRET in laser scanning confocal, by sensitized emission. One of them is to correct misfocusing deviation (page 2521). This error comes from two main sources. One is chromatic aberration, which we are quite familiar. The other is the slight differences in the collimation of the laser beams.



Any comments?

Are there ways to test the performance of the system?

Thanks



Hao-Yang Liu

[Michael Cammer]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Suggest testing the system with fine Z series of 0.1 um multicolor beads.

>
> In a paper, Biophysical Journal, vol 86, p2517, 2004, the work mentioned
> several factors needed to be corrected when perform FRET in laser scanning
> confocal, by sensitized emission. One of them is to correct misfocusing
> deviation (page 2521). This error comes from two main sources. One is
> chromatic aberration, which we are quite familiar. The other is the slight
> differences in the collimation of the laser beams.
>
>
>
> Any comments?
>
> Are there ways to test the performance of the system?
>
> Thanks
>
>
>
> Hao-Yang Liu
>

_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/

[david anchel]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi there,

I too have the same problem with aligning the Z focus of my NIR and VIS lasers.  Where can we get these .1um multicolour beads?

Dave Anchel,
Toronto

<html><DIV>
<DIV></DIV></DIV></html>

> Date: Thu, 1 May 2008 17:27:43 -0400
> From: [hidden email]
> Subject: Re: Correcting misfocusing deviation in confocal FRET imaging.
> To: [hidden email]
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Suggest testing the system with fine Z series of 0.1 um multicolor beads.
>
> >
> > In a paper, Biophysical Journal, vol 86, p2517, 2004, the work mentioned
> > several factors needed to be corrected when perform FRET in laser scanning
> > confocal, by sensitized emission. One of them is to correct misfocusing
> > deviation (page 2521). This error comes from two main sources. One is
> > chromatic aberration, which we are quite familiar. The other is the slight
> > differences in the collimation of the laser beams.
> >
> >
> >
> > Any comments?
> >
> > Are there ways to test the performance of the system?
> >
> > Thanks
> >
> >
> >
> > Hao-Yang Liu
> >
>
>
> _________________________________________
> Michael Cammer http://www.aecom.yu.edu/aif/

---

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[Michael Weber-4]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

David,

you can get 0.17 µm multicolor beads from Invitrogen. There size is
below the common resolution limit of light microscopy, so you can use
them to test and calibrate your setup. Get them in solution and make
your own slides, the prepared ones are quite expensive.

Michael


david anchel wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi there,
>
> I too have the same problem with aligning the Z focus of my NIR and VIS
> lasers.  Where can we get these .1um multicolour beads?
>
> Dave Anchel,
> Toronto
>
> <html><DIV>
> <DIV></DIV></DIV></html>
>
>  > Date: Thu, 1 May 2008 17:27:43 -0400
>  > From: [hidden email]
>  > Subject: Re: Correcting misfocusing deviation in confocal FRET imaging.
>  > To: [hidden email]
>  >
>  > Search the CONFOCAL archive at
>  > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>  >
>  > Suggest testing the system with fine Z series of 0.1 um multicolor beads.
>  >
>  > >
>  > > In a paper, Biophysical Journal, vol 86, p2517, 2004, the work
> mentioned
>  > > several factors needed to be corrected when perform FRET in laser
> scanning
>  > > confocal, by sensitized emission. One of them is to correct misfocusing
>  > > deviation (page 2521). This error comes from two main sources. One is
>  > > chromatic aberration, which we are quite familiar. The other is the
> slight
>  > > differences in the collimation of the laser beams.
>  > >
>  > >
>  > >
>  > > Any comments?
>  > >
>  > > Are there ways to test the performance of the system?
>  > >
>  > > Thanks
>  > >
>  > >
>  > >
>  > > Hao-Yang Liu
>  > >
>  >
>  >
>  > _________________________________________
>  > Michael Cammer http://www.aecom.yu.edu/aif/
>
> ------------------------------------------------------------------------
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