[Job Posting] Bitplane Regional Sales Engineer
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Daniel McRitchie |
[Job Posting] Bitplane Regional Sales Engineer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Sales of Imaris software within France, Switzerland, BeNeLux. KEY TASKS AND DUTIES: 1. Meet/Exceed agreed team and individual sales targets 2. Customer visits and analysis of customers imaging needs 3. Acquisition of new customers 4. Organisation of exhibitions and workshops 5. Support and strengthen existing distributors relationships & highlight potential new distributors within the defined territory 6. Any other duties as may be reasonably required from time to time by your line manager Full details available at http://www.bitplane.com/go/bitplane/jobs |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Bonjour à tous, I would like to have some suggestions in order to buy a power meter for checking the LASERs intensities at the objective level. Thanks for your help! Louis Louis Villeneuve Research Associate - Confocal Microscopy Montreal Heart Institute- Research Center 5000 East Belanger Montreal (Qc), Canada H1T 1C8 514-376-3330 ext 3511 514-376-1355 (Fax) [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Bonjour Louis, No commercial association but I have been very happy with Lumen Dynamics' XP750 (http://www.ldgi-xcite.com/products-xr2100-xp750.php) for that exact purpose. Bonne chance, - Damir On 5/24/2012 7:22 AM, [hidden email] wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Bonjour à tous, > > I would like to have some suggestions in order to buy a power meter for > checking the LASERs intensities at the objective level. > > Thanks for your help! > > Louis > > Louis Villeneuve > Research Associate - Confocal Microscopy > Montreal Heart Institute- Research Center > 5000 East Belanger > Montreal (Qc), Canada > H1T 1C8 > > 514-376-3330 ext 3511 > 514-376-1355 (Fax) > > [hidden email] -- Damir Sudar - Staff Scientist and Deputy for Technology Lawrence Berkeley Laboratory / Life Sciences Division One Cyclotron Road, MS 977, Berkeley, CA 94720, USA T: 510/486-5346 - F: 510/486-5586 - E: [hidden email] WWW: http://www.lbl.gov/lsd/People_&_Organization/Scientific_Staff_Directory/Sudar_Lab.html |
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In reply to this post by Louis Villeneuve
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Louis, you might consider this product from Lumen dynamics (no commercial interest): http://www.ldgi-xcite.com/products-xr2100-xp750.php A regular power meter will work as well provided you can hold it in a fixed position above your objective. Just be aware that power meters like these have a certain acceptance angle and won't work with high NA or immersion objectives. You're better off measuring the power at the nosepiece after removing the objective of interest and then calculating the resulting power that gets through the objective based on it's wavelength transmission curve in those cases. Also be aware that these devices don't tell over what area your power is projected in or extending beyond your field of view. There other ways and devices you can use to determine that. Cheers John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada www.spectral.ca On 2012-05-24, at 10:22 AM, [hidden email] wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Bonjour à tous, > > I would like to have some suggestions in order to buy a power meter for > checking the LASERs intensities at the objective level. > > Thanks for your help! > > Louis > > Louis Villeneuve > Research Associate - Confocal Microscopy > Montreal Heart Institute- Research Center > 5000 East Belanger > Montreal (Qc), Canada > H1T 1C8 > > 514-376-3330 ext 3511 > 514-376-1355 (Fax) > > [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >***** > >Hi Louis, you might consider this product from >Lumen dynamics (no commercial interest): > >http://www.ldgi-xcite.com/products-xr2100-xp750.php > >A regular power meter will work as well provided >you can hold it in a fixed position above your >objective. > >Just be aware that power meters like these have >a certain acceptance angle and won't work with >high NA or immersion objectives. You're better >off measuring the power at the nosepiece after >removing the objective of interest and then >calculating the resulting power that gets >through the objective based on it's wavelength >transmission curve in those cases. >Also be aware that these devices don't tell over >what area your power is projected in or >extending beyond your field of view. There other >ways and devices you can use to determine that. > >Cheers > > >John Oreopoulos >Research Assistant >Spectral Applied Research >Richmond Hill, Ontario >Canada >www.spectral.ca I agree with John about the problems of "getting the light out of high-NA immersion lenses and into the sensitive area of the power meter", but we need to add that not all of the light getting to the nosepiece will actually enter the entrance pupil of the objective. So you need to compensate for the NA and magnification of the objective as well as its transmission. If the illumination optics have been designed to "fill" (Fuzzy definition here: How uniforml or peaked is this light bundle?) a 40x 1.2, then you switch to a 100x 1.2, the area of the pupil of the latter will be 6.25x smaller. Depending on how uniform (or peaked) the laser light is in the BFP, this may mean that the light striking the glass part of the 40x will represent about 5x more photons than that striking the glass part of the 100x. This should be remembered when setting the Laser power level. And also when comparing the overall performance of 40x, 63x and 100x lenses (The differences you thought that you saw may be mostly due to more power striking the specimen at 40x), Cheers, Jim Pawley > > >On 2012-05-24, at 10:22 AM, [hidden email] wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Bonjour à tous, >> >> I would like to have some suggestions in order to buy a power meter for >> checking the LASERs intensities at the objective level. >> >> Thanks for your help! >> >> Louis >> >> Louis Villeneuve >> Research Associate - Confocal Microscopy >> Montreal Heart Institute- Research Center >> 5000 East Belanger >> Montreal (Qc), Canada >> H1T 1C8 >> >> 514-376-3330 ext 3511 >> 514-376-1355 (Fax) >> >> [hidden email] -- James and Christine Pawley, 21 N. Prospect Ave. Madison, WI, 53726 Phone: 608-238-3953 <[hidden email]> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** To get the full power at the back aperture I check the Laser power using a 25mm diameter F100mm lens mounted in a 1" SMS tube with an SMS-RMS thread adapter (Thor Labs). I place the nose piece in the lowest position, replace my objective with the F100mm lens assembly and use the X-Cite power meter (XR2100) with the XP750 slide mounted sensor for my measurements. I repeat this with the objective of interest after I focus on a specimen. Make sure the brightfield condenser is centered so that you can align the sensor over the objective. I have no commercial interest in Thor Labs or X-Cite Eric Marino Senior Imaging Specialist Immune Disease Institute Harvard Medical School 200 Longwood Ave WAB 133D Boston, MA 02115 Lab: 617 713-8885 [hidden email] On May 24, 2012, at 6:08 PM, James Pawley wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi Louis, you might consider this product from Lumen dynamics (no commercial interest): >> >> http://www.ldgi-xcite.com/products-xr2100-xp750.php >> >> A regular power meter will work as well provided you can hold it in a fixed position above your objective. >> >> Just be aware that power meters like these have a certain acceptance angle and won't work with high NA or immersion objectives. You're better off measuring the power at the nosepiece after removing the objective of interest and then calculating the resulting power that gets through the objective based on it's wavelength transmission curve in those cases. >> Also be aware that these devices don't tell over what area your power is projected in or extending beyond your field of view. There other ways and devices you can use to determine that. >> >> Cheers >> >> >> John Oreopoulos >> Research Assistant >> Spectral Applied Research >> Richmond Hill, Ontario >> Canada >> www.spectral.ca > > > I agree with John about the problems of "getting the light out of high-NA immersion lenses and into the sensitive area of the power meter", but we need to add that not all of the light getting to the nosepiece will actually enter the entrance pupil of the objective. > > So you need to compensate for the NA and magnification of the objective as well as its transmission. > > If the illumination optics have been designed to "fill" (Fuzzy definition here: How uniforml or peaked is this light bundle?) a 40x 1.2, then you switch to a 100x 1.2, the area of the pupil of the latter will be 6.25x smaller. Depending on how uniform (or peaked) the laser light is in the BFP, this may mean that the light striking the glass part of the 40x will represent about 5x more photons than that striking the glass part of the 100x. > > This should be remembered when setting the Laser power level. And also when comparing the overall performance of 40x, 63x and 100x lenses (The differences you thought that you saw may be mostly due to more power striking the specimen at 40x), > > Cheers, > > Jim Pawley > > >> >> >> On 2012-05-24, at 10:22 AM, [hidden email] wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> ***** >>> >>> Bonjour à tous, >>> >>> I would like to have some suggestions in order to buy a power meter for >>> checking the LASERs intensities at the objective level. >>> >>> Thanks for your help! >>> >>> Louis >>> >>> Louis Villeneuve >>> Research Associate - Confocal Microscopy >>> Montreal Heart Institute- Research Center >>> 5000 East Belanger >>> Montreal (Qc), Canada >>> H1T 1C8 >>> >>> 514-376-3330 ext 3511 >>> 514-376-1355 (Fax) >>> >>> [hidden email] > > > -- > James and Christine Pawley, 21 N. Prospect Ave. Madison, WI, 53726 Phone: 608-238-3953 > <[hidden email]> >
Eric Marino
Senior Imaging Specialist Program in Cellular and Molecular Medicine Boston Children's Hospital |
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In reply to this post by Louis Villeneuve
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Bonjour Louis A few points that might be of interest: Which meter you use depends on what you are interested in measuring: do you want to compare laser power with different objectives, check if the performance is stable from let's say one month to the next, see if you can trust your overnight data or measure usec variations for laser studies? If you have an upright microscope make sure you have enough space between the stage and the objective to insert the probe. They are often quite high. The one from Lumen isn't. You also need to decide if you want to record the data or if it is enough for you to see the fluctuations on the display. The Lumen Dynamics power meter only records data when they come out of their ExCite lamp (using a separate input) so if you want to record data from lasers using the stage probe, you need to be there to press a button at every time point. Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of > [hidden email] > Sent: 24 May 2012 16:22 > To: [hidden email] > Subject: Power Meter > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Bonjour à tous, > > I would like to have some suggestions in order to buy a power meter for checking > the LASERs intensities at the objective level. > > Thanks for your help! > > Louis > > Louis Villeneuve > Research Associate - Confocal Microscopy Montreal Heart Institute- Research > Center > 5000 East Belanger > Montreal (Qc), Canada > H1T 1C8 > > 514-376-3330 ext 3511 > 514-376-1355 (Fax) > > [hidden email] |
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In reply to this post by Louis Villeneuve
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** For power measurements at the nosepiece, you could measure the entrance pupil of the particular objective, then punch a hole of the same diameter in a piece of black cardboard or plastic and tape this over the mounting hole in the nosepiece, making sure it is well centered. This way, your meter is getting about the same amount of light as would enter the objective. As suggested earlier in this thread, one would then correct this measurement according tho the objective's transmission curve for the transmission losses. Stan Vitha Microscopy and Imaging Center Texas A&M University On Thu, 24 May 2012 17:08:20 -0500, James Pawley <[hidden email]> wrote: > >I agree with John about the problems of "getting >the light out of high-NA immersion lenses and >into the sensitive area of the power meter", but >we need to add that not all of the light getting >to the nosepiece will actually enter the entrance >pupil of the objective. > >So you need to compensate for the NA and >magnification of the objective as well as its >transmission. > >If the illumination optics have been designed to >"fill" (Fuzzy definition here: How uniforml or >peaked is this light bundle?) a 40x 1.2, then you >switch to a 100x 1.2, the area of the pupil of >the latter will be 6.25x smaller. Depending on >how uniform (or peaked) the laser light is in the >BFP, this may mean that the light striking the >glass part of the 40x will represent about 5x >more photons than that striking the glass part of >the 100x. > >This should be remembered when setting the Laser >power level. And also when comparing the overall >performance of 40x, 63x and 100x lenses (The >differences you thought that you saw may be >mostly due to more power striking the specimen at >40x), > >Cheers, > >Jim Pawley > > > >-- >James and Christine Pawley, 21 N. Prospect Ave. >Madison, WI, 53726 Phone: 608-238-3953 ><[hidden email]> |
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