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Kohler equivalent

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Monique Vasseur Monique Vasseur
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Kohler equivalent

Hi everyone,

 

To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?   

 

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC    H3C 3J7   Canada

tél. (514) 343-6111 poste 5148
Goodhouse, Joseph G. Goodhouse, Joseph G.
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Re: Kohler equivalent

                I suppose you could do this. When the condenser is focused it will be where you have the brightest illumination. With  the iris closed down you could determine whether or not he condenser is on axis with the objective lens.

 

Joe Goodhouse
Confocal Core Lab Manager
Dept. of Molecular Biology
Princeton University

Washington Road

Princeton, NJ. 08544-1014
609-258-5432

Visit us at http://www.molbio1.princeton.edu/facility/confocal/

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vasseur Monique
Sent: Thursday, January 21, 2010 12:41 PM
To: [hidden email]
Subject: Kohler equivalent

 

Hi everyone,

 

To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?   

 

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC    H3C 3J7   Canada

tél. (514) 343-6111 poste 5148
Dale Callaham Dale Callaham
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Re: Kohler equivalent

In reply to this post by Monique Vasseur
You will need to establish the back conjugate of the specimen plane and
adjust the condenser focus in the normal Kohler alignment method; but is
it accessible and how do you define it in an unobtrusive way without an
actual field diaphragm?

If you need precise Kohler illumination you just might need a better
microscope......

Dale

Vasseur Monique wrote:

> Hi everyone,
>
>
>
> To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?
>
>
>
> Monique Vasseur
>
> Microscopie et imagerie
>
> Département de biochimie
>
> Université de Montréal
>
> C.P. 6128, succursale Centre-ville
>
> Montréal QC    H3C 3J7   Canada
>
> tél. (514) 343-6111 poste 5148
>
Guy Cox Guy Cox
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Re: Kohler equivalent

In reply to this post by Monique Vasseur

The other responses were a bit gloomy – in fact it’s quite easy.   Look down the tube (without an eyepiece) and adjust the condenser height until the condenser iris does what it’s supposed to do.   That is, when  you open it fully the whole of the bfp is illuminated and as you close it the illuminated area decreases smoothly, with a sharp edge, down to a little spot.  (You could use a phase telescope for this but it can be a bit fiddly knowing where to focus it.)  You will find that if the condenser isn’t focussed  correctly you will not be able to fill the bfp, and you will see strange effects such a dark patch in the middle as you close it.   It’s really easier to do than to explain!

 

                                                                                                Guy

 

Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Electron Microscope Unit, Madsen Building F09,

University of Sydney, NSW 2006

 

Phone +61 2 9351 3176     Fax +61 2 9351 7682

             Mobile 0413 281 861

______________________________________________

      http://www.guycox.net

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vasseur Monique
Sent: Friday, 22 January 2010 4:41 AM
To: [hidden email]
Subject: Kohler equivalent

 

Hi everyone,

 

To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?   

 

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC    H3C 3J7   Canada

tél. (514) 343-6111 poste 5148

No virus found in this incoming message.
Checked by AVG - www.avg.com
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Michael Chua Michael Chua
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Re: Kohler equivalent

And of course an elegant way of focussing the condensor aperture is to use a Bertrand lens, which tends to be included with many good quality scope setups.   Removing the eye piece as Guy describes is a cheep alternative to a Bertrand lens!

Michael
919-943-3268
[hidden email]
http://microscopy.unc.edu
Michael Hooker Microscopy Facility



On Jan 21, 2010, at 7:54 PM, Guy Cox <[hidden email]> wrote:

The other responses were a bit gloomy – in fact it’s quite easy.   Look down the tube (without an eyepiece) and adjust the condenser height until the condenser iris does what it’s supposed to do.   That is, when  you open it fully the whole of the bfp is illuminated and as you close it the illuminated area decreases smoothly, with a sharp edge, down to a little spot.  (You could use a phase telescope for this but it can be a bit fiddly knowing where to focus it.)  You will find that if the condenser isn’t focussed  correctly you will not be able to fill the bfp, and you will see strange effects such a dark patch in the middle as you close it.   It’s really easier to do than to explain!

 

                                                                                                Guy

 

Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Electron Microscope Unit, Madsen Building F09,

University of Sydney, NSW 2006

 

Phone +61 2 9351 3176     Fax +61 2 9351 7682

             Mobile 0413 281 861

______________________________________________

      http://www.guycox.net

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vasseur Monique
Sent: Friday, 22 January 2010 4:41 AM
To: [hidden email]
Subject: Kohler equivalent

 

Hi everyone,

 

To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?   

 

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC    H3C 3J7   Canada

tél. (514) 343-6111 poste 5148

No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date: 01/21/10 06:18:00
Guy Cox Guy Cox
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Re: Kohler equivalent

I was assuming that a microscope which was basic enough not to have a field iris was unlikely to have a Bertrand lens!

                                     Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006

Phone +61 2 9351 3176     Fax +61 2 9351 7682
             Mobile 0413 281 861
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MChua
Sent: Friday, 22 January 2010 12:51 PM
To: [hidden email]
Subject: Re: Kohler equivalent

And of course an elegant way of focussing the condensor aperture is to  
use a Bertrand lens, which tends to be included with many good quality  
scope setups.   Removing the eye piece as Guy describes is a cheep  
alternative to a Bertrand lens!

Michael
919-943-3268
[hidden email]
http://microscopy.unc.edu
Michael Hooker Microscopy Facility



On Jan 21, 2010, at 7:54 PM, Guy Cox <[hidden email]> wrote:

> The other responses were a bit gloomy – in fact it’s quite  
> easy.   Look down the tube (without an eyepiece) and adjust the cond
> enser height until the condenser iris does what it’s supposed to do.
>    That is, when  you open it fully the whole of the bfp is illumina
> ted and as you close it the illuminated area decreases smoothly, wit
> h a sharp edge, down to a little spot.  (You could use a phase teles
> cope for this but it can be a bit fiddly knowing where to focus it.)
>   You will find that if the condenser isn’t focussed  correctly you  
> will not be able to fill the bfp, and you will see strange effects s
> uch a dark patch in the middle as you close it.   It’s really easier
>  to do than to explain!
>
>
>
>    
>    
>    
>    
>    
>    
>    
>    
>    
>                                                                      
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
>
> by Guy Cox    CRC Press / Taylor & Francis
>
>      http://www.guycox.com/optical.htm
>
> ______________________________________________
>
> Associate Professor Guy Cox, MA, DPhil(Oxon)
>
> Electron Microscope Unit, Madsen Building F09,
>
> University of Sydney, NSW 2006
>
>
>
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>
>              Mobile 0413 281 861
>
> ______________________________________________
>
>       http://www.guycox.net
>
>
>
>
>
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On Behalf Of Vasseur Monique
> Sent: Friday, 22 January 2010 4:41 AM
> To: [hidden email]
> Subject: Kohler equivalent
>
>
>
> Hi everyone,
>
>
>
> To do Kohler illumination alignment, we need a field diaphragm and a  
> condenser on the microscope.  What about the ones that have only a  
> condenser with adjustable aperture, and no field diaphragm;  is  
> there a kind of standard method to properly adjust the height of the  
> condenser, for upright and inverted microscopes?
>
>
>
> Monique Vasseur
>
> Microscopie et imagerie
>
> Département de biochimie
>
> Université de Montréal
>
> C.P. 6128, succursale Centre-ville
>
> Montréal QC    H3C 3J7   Canada
>
> tél. (514) 343-6111 poste 5148
>
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
> Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date:  
> 01/21/10 06:18:00

No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 9.0.730 / Virus Database: 271.1.1/2637 - Release Date: 01/22/10 06:34:00
Monique Vasseur Monique Vasseur
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Re: Kohler equivalent

In reply to this post by Guy Cox

Thanks a lot, I appreciate your answers.

 

Monique Vasseur

De : Confocal Microscopy List [mailto:[hidden email]] De la part de Guy Cox
Envoyé : 21 janvier 2010 19:54
À : [hidden email]
Objet : Re: Kohler equivalent

 

The other responses were a bit gloomy – in fact it’s quite easy.   Look down the tube (without an eyepiece) and adjust the condenser height until the condenser iris does what it’s supposed to do.   That is, when  you open it fully the whole of the bfp is illuminated and as you close it the illuminated area decreases smoothly, with a sharp edge, down to a little spot.  (You could use a phase telescope for this but it can be a bit fiddly knowing where to focus it.)  You will find that if the condenser isn’t focussed  correctly you will not be able to fill the bfp, and you will see strange effects such a dark patch in the middle as you close it.   It’s really easier to do than to explain!

 

                                                                                                Guy

 

Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Electron Microscope Unit, Madsen Building F09,

University of Sydney, NSW 2006

 

Phone +61 2 9351 3176     Fax +61 2 9351 7682

             Mobile 0413 281 861

______________________________________________

      http://www.guycox.net

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vasseur Monique
Sent: Friday, 22 January 2010 4:41 AM
To: [hidden email]
Subject: Kohler equivalent

 

Hi everyone,

 

To do Kohler illumination alignment, we need a field diaphragm and a condenser on the microscope.  What about the ones that have only a condenser with adjustable aperture, and no field diaphragm;  is there a kind of standard method to properly adjust the height of the condenser, for upright and inverted microscopes?   

 

Monique Vasseur

Microscopie et imagerie

Département de biochimie

Université de Montréal

C.P. 6128, succursale Centre-ville

Montréal QC    H3C 3J7   Canada

tél. (514) 343-6111 poste 5148

No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date: 01/21/10 06:18:00
Peter Gabriel Pitrone Peter Gabriel Pitrone
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Re: Kohler equivalent

In reply to this post by Guy Cox
Hello Guy,

Another "cheap" way to do this with out a Bertrand lanes is to punch a pinhole through an eyepiece cover that comes with the scope. it serves as a "poor mans" telescope. Have you ever heard of anybody doing this?

Pete

On Jan 22, 2010, at 02:58 AM, Guy Cox wrote:

> I was assuming that a microscope which was basic enough not to have a field iris was unlikely to have a Bertrand lens!
>
>                                     Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>     http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>             Mobile 0413 281 861
> ______________________________________________
>      http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MChua
> Sent: Friday, 22 January 2010 12:51 PM
> To: [hidden email]
> Subject: Re: Kohler equivalent
>
> And of course an elegant way of focussing the condensor aperture is to  
> use a Bertrand lens, which tends to be included with many good quality  
> scope setups.   Removing the eye piece as Guy describes is a cheep  
> alternative to a Bertrand lens!
>
> Michael
> 919-943-3268
> [hidden email]
> http://microscopy.unc.edu
> Michael Hooker Microscopy Facility
>
>
>
> On Jan 21, 2010, at 7:54 PM, Guy Cox <[hidden email]> wrote:
>
>> The other responses were a bit gloomy – in fact it’s quite  
>> easy.   Look down the tube (without an eyepiece) and adjust the cond
>> enser height until the condenser iris does what it’s supposed to do.
>>   That is, when  you open it fully the whole of the bfp is illumina
>> ted and as you close it the illuminated area decreases smoothly, wit
>> h a sharp edge, down to a little spot.  (You could use a phase teles
>> cope for this but it can be a bit fiddly knowing where to focus it.)
>>  You will find that if the condenser isn’t focussed  correctly you  
>> will not be able to fill the bfp, and you will see strange effects s
>> uch a dark patch in the middle as you close it.   It’s really easier
>> to do than to explain!
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>>
>> by Guy Cox    CRC Press / Taylor & Francis
>>
>>     http://www.guycox.com/optical.htm
>>
>> ______________________________________________
>>
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>
>> Electron Microscope Unit, Madsen Building F09,
>>
>> University of Sydney, NSW 2006
>>
>>
>>
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>>
>>             Mobile 0413 281 861
>>
>> ______________________________________________
>>
>>      http://www.guycox.net
>>
>>
>>
>>
>>
>> From: Confocal Microscopy List  
>> [mailto:[hidden email]] On Behalf Of Vasseur Monique
>> Sent: Friday, 22 January 2010 4:41 AM
>> To: [hidden email]
>> Subject: Kohler equivalent
>>
>>
>>
>> Hi everyone,
>>
>>
>>
>> To do Kohler illumination alignment, we need a field diaphragm and a  
>> condenser on the microscope.  What about the ones that have only a  
>> condenser with adjustable aperture, and no field diaphragm;  is  
>> there a kind of standard method to properly adjust the height of the  
>> condenser, for upright and inverted microscopes?
>>
>>
>>
>> Monique Vasseur
>>
>> Microscopie et imagerie
>>
>> Département de biochimie
>>
>> Université de Montréal
>>
>> C.P. 6128, succursale Centre-ville
>>
>> Montréal QC    H3C 3J7   Canada
>>
>> tél. (514) 343-6111 poste 5148
>>
>> No virus found in this incoming message.
>> Checked by AVG - www.avg.com
>> Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date:  
>> 01/21/10 06:18:00
>
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
> Version: 9.0.730 / Virus Database: 271.1.1/2637 - Release Date: 01/22/10 06:34:00
Guy Cox-2 Guy Cox-2
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Re: Kohler equivalent

That's a new one to me.  Optically, I can understand that it could work, but I'll have to give it a try!

                                   Guy





-----Original Message-----


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter Pitrone
Sent: Wednesday, 3 March 2010 9:03 PM
To: [hidden email]
Subject: Re: Kohler equivalent

Hello Guy,

Another "cheap" way to do this with out a Bertrand lanes is to punch a pinhole through an eyepiece cover that comes with the scope. it serves as a "poor mans" telescope. Have you ever heard of anybody doing this?

Pete

On Jan 22, 2010, at 02:58 AM, Guy Cox wrote:

> I was assuming that a microscope which was basic enough not to have a field iris was unlikely to have a Bertrand lens!
>
>                                     Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>     http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>             Mobile 0413 281 861
> ______________________________________________
>      http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MChua
> Sent: Friday, 22 January 2010 12:51 PM
> To: [hidden email]
> Subject: Re: Kohler equivalent
>
> And of course an elegant way of focussing the condensor aperture is to  
> use a Bertrand lens, which tends to be included with many good quality  
> scope setups.   Removing the eye piece as Guy describes is a cheep  
> alternative to a Bertrand lens!
>
> Michael
> 919-943-3268
> [hidden email]
> http://microscopy.unc.edu
> Michael Hooker Microscopy Facility
>
>
>
> On Jan 21, 2010, at 7:54 PM, Guy Cox <[hidden email]> wrote:
>
>> The other responses were a bit gloomy - in fact it's quite  
>> easy.   Look down the tube (without an eyepiece) and adjust the cond
>> enser height until the condenser iris does what it's supposed to do.
>>   That is, when  you open it fully the whole of the bfp is illumina
>> ted and as you close it the illuminated area decreases smoothly, wit
>> h a sharp edge, down to a little spot.  (You could use a phase teles
>> cope for this but it can be a bit fiddly knowing where to focus it.)
>>  You will find that if the condenser isn't focussed  correctly you  
>> will not be able to fill the bfp, and you will see strange effects s
>> uch a dark patch in the middle as you close it.   It's really easier
>> to do than to explain!
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>>
>> by Guy Cox    CRC Press / Taylor & Francis
>>
>>     http://www.guycox.com/optical.htm
>>
>> ______________________________________________
>>
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>
>> Electron Microscope Unit, Madsen Building F09,
>>
>> University of Sydney, NSW 2006
>>
>>
>>
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>>
>>             Mobile 0413 281 861
>>
>> ______________________________________________
>>
>>      http://www.guycox.net
>>
>>
>>
>>
>>
>> From: Confocal Microscopy List  
>> [mailto:[hidden email]] On Behalf Of Vasseur Monique
>> Sent: Friday, 22 January 2010 4:41 AM
>> To: [hidden email]
>> Subject: Kohler equivalent
>>
>>
>>
>> Hi everyone,
>>
>>
>>
>> To do Kohler illumination alignment, we need a field diaphragm and a  
>> condenser on the microscope.  What about the ones that have only a  
>> condenser with adjustable aperture, and no field diaphragm;  is  
>> there a kind of standard method to properly adjust the height of the  
>> condenser, for upright and inverted microscopes?
>>
>>
>>
>> Monique Vasseur
>>
>> Microscopie et imagerie
>>
>> Département de biochimie
>>
>> Université de Montréal
>>
>> C.P. 6128, succursale Centre-ville
>>
>> Montréal QC    H3C 3J7   Canada
>>
>> tél. (514) 343-6111 poste 5148
>>
>> No virus found in this incoming message.
>> Checked by AVG - www.avg.com
>> Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date:  
>> 01/21/10 06:18:00
>
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
> Version: 9.0.730 / Virus Database: 271.1.1/2637 - Release Date: 01/22/10 06:34:00

No virus found in this incoming message.
Checked by AVG - www.avg.com
Version: 9.0.733 / Virus Database: 271.1.1/2710 - Release Date: 03/03/10 06:34:00
Julian Smith III Julian Smith III
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Re: Kohler equivalent

In reply to this post by Peter Gabriel Pitrone
Pinhole eyecap--poor-man's substitute for a Bertrand lens.
Described in Peter Gray's "Handbook of Basic Microtechnique", and the
old A/O Ortho-illuminator came with one handily screwed onto one of the
mounting bolts....
Julian

Peter Pitrone wrote:

> Hello Guy,
>
> Another "cheap" way to do this with out a Bertrand lanes is to punch a pinhole through an eyepiece cover that comes with the scope. it serves as a "poor mans" telescope. Have you ever heard of anybody doing this?
>
> Pete
>
> On Jan 22, 2010, at 02:58 AM, Guy Cox wrote:
>
>  
>> I was assuming that a microscope which was basic enough not to have a field iris was unlikely to have a Bertrand lens!
>>
>>                                     Guy
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox    CRC Press / Taylor & Francis
>>     http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>>
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>>             Mobile 0413 281 861
>> ______________________________________________
>>      http://www.guycox.net
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MChua
>> Sent: Friday, 22 January 2010 12:51 PM
>> To: [hidden email]
>> Subject: Re: Kohler equivalent
>>
>> And of course an elegant way of focussing the condensor aperture is to  
>> use a Bertrand lens, which tends to be included with many good quality  
>> scope setups.   Removing the eye piece as Guy describes is a cheep  
>> alternative to a Bertrand lens!
>>
>> Michael
>> 919-943-3268
>> [hidden email]
>> http://microscopy.unc.edu
>> Michael Hooker Microscopy Facility
>>
>>
>>
>> On Jan 21, 2010, at 7:54 PM, Guy Cox <[hidden email]> wrote:
>>
>>    
>>> The other responses were a bit gloomy – in fact it’s quite  
>>> easy.   Look down the tube (without an eyepiece) and adjust the cond
>>> enser height until the condenser iris does what it’s supposed to do.
>>>   That is, when  you open it fully the whole of the bfp is illumina
>>> ted and as you close it the illuminated area decreases smoothly, wit
>>> h a sharp edge, down to a little spot.  (You could use a phase teles
>>> cope for this but it can be a bit fiddly knowing where to focus it.)
>>>  You will find that if the condenser isn’t focussed  correctly you  
>>> will not be able to fill the bfp, and you will see strange effects s
>>> uch a dark patch in the middle as you close it.   It’s really easier
>>> to do than to explain!
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>> Guy
>>>
>>>
>>>
>>> Optical Imaging Techniques in Cell Biology
>>>
>>> by Guy Cox    CRC Press / Taylor & Francis
>>>
>>>     http://www.guycox.com/optical.htm
>>>
>>> ______________________________________________
>>>
>>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>>
>>> Electron Microscope Unit, Madsen Building F09,
>>>
>>> University of Sydney, NSW 2006
>>>
>>>
>>>
>>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>>>
>>>             Mobile 0413 281 861
>>>
>>> ______________________________________________
>>>
>>>      http://www.guycox.net
>>>
>>>
>>>
>>>
>>>
>>> From: Confocal Microscopy List  
>>> [mailto:[hidden email]] On Behalf Of Vasseur Monique
>>> Sent: Friday, 22 January 2010 4:41 AM
>>> To: [hidden email]
>>> Subject: Kohler equivalent
>>>
>>>
>>>
>>> Hi everyone,
>>>
>>>
>>>
>>> To do Kohler illumination alignment, we need a field diaphragm and a  
>>> condenser on the microscope.  What about the ones that have only a  
>>> condenser with adjustable aperture, and no field diaphragm;  is  
>>> there a kind of standard method to properly adjust the height of the  
>>> condenser, for upright and inverted microscopes?
>>>
>>>
>>>
>>> Monique Vasseur
>>>
>>> Microscopie et imagerie
>>>
>>> Département de biochimie
>>>
>>> Université de Montréal
>>>
>>> C.P. 6128, succursale Centre-ville
>>>
>>> Montréal QC    H3C 3J7   Canada
>>>
>>> tél. (514) 343-6111 poste 5148
>>>
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>>> Checked by AVG - www.avg.com
>>> Version: 9.0.730 / Virus Database: 270.14.122/2590 - Release Date:  
>>> 01/21/10 06:18:00
>>>      
>> No virus found in this incoming message.
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>>    
>
>  


--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
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