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Hi
A complicated question. As a buffer, fluo-4 will affect Ca cycling and Ca cycling is implicated in SA nodal discharge rate (See Lakatta group papers for a start)(etc.). Focussed laser light can damage cells as well. In experiments one tries to minimise such effects… So the problem boils down to if you don’t affect the system in some way you can’t measure anything.
Not sure if this helps.
Regards Mark
On 6/02/2014, at 1:39 pm, Louis Villeneuve <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi everyone,
>
> Does Fluo 4 and/or the 488 nm Argon Laser line could contribute to slow the
> rate of the spontaneous transient in heart myocyte (ventricular, atrial, SAN)?
>
> Does anyone have read or notice that event in their own experiment?
> References would be appreciated also!!!!
>
> Thanks to all for your help!!!!
>
> Louis
>
>
> Louis Villeneuve
> Confocal microscopy manager
> Montreal Heart Institute
Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology & Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK
[hidden email]
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