LM Facility Managers Meeting 2017 - Registration now open
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Mel Reedman-2 |
LM Facility Managers Meeting 2017 - Registration now open
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear All Registration for the 2017 LM Facility Managers Meeting, being held at the Royal York Hotel, York, UK, is now open. www.rms.orgk.uk/lm-facility-managers Scientific Organisers: Peter O'Toole and Jo Marrison 5 - 6 January 2017 ‘The next Facility Managers Meeting aimed at people running or working in light microscopy facilities will be held in York. This will be the 10th Facility Managers Meeting. From very humble beginnings, we have grown to a much more significant and influential group. Numbers of attendees have grown 10 fold over this time as more and more facilities have opened up. We now represent one of the best organised facility groupings in the UK if not indeed the world. At York, we there will be a session on the latest developments in UK Bioimaging and how we can feed in to wider international groups that are starting up. We will also discuss some of the basic elements (funding, impact measures) of running a core facility as well as the latest technological and application developments that effect ourselves and our users. We look forward to welcoming you all at York.’ – Peter O’Toole and Jo Marrison. This event regularly attracts around 100 facility managers and colleagues from all over the country and provides an opportunity for imaging managers to interact, discuss issues and share ideas. The meeting is informal and free discussion is encouraged. Participation from commercial organisations, microscope manufacturers and software developers is welcomed. The event is an ideal opportunity to exchange feedback and to hear about new technologies. Registration Registration to the 2 day meeting is FREE for academic attendees. The costs of running this meeting and your attendance is paid for by the generous support of the commercial representatives attending the meeting. To register for this meeting, please visit www.rms.org.uk/lm-facility-managers Accommodation at the Royal York Hotel We have secured a number of rooms at the Royal York Hotel, which has been recently refurbished to a high standard. Rooms are £100 per night including breakfast. The hotel is convenient to travel to, being located adjacent to York train station and also a number of pay and display car parks. The meeting is taking place fully within the hotel to encourage networking and maximise the opportunities for discussion. If you wish to book accommodation for the night of 5 January, please select the registration option, ‘Academic rate incl. accommodation’ on the registration page. To register and select accommodation, please visit www.rms.org.uk/lm-facility-managers. An outline programme is now available to view on the RMS event page www.rms.org.uk/lm-facility-managers This meeting often fills up quickly so we recommend booking your place well in advance. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! Tara Capece University of Rochester |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** You may use sub resolution latex beads (tetraspec) from molecular probes measure XY and Z resolution with the help of FWHM test. Reg Ganesh Sent from my iPhone > On 09-Nov-2016, at 7:22 PM, Capece, Tara <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! > > Tara Capece > University of Rochester |
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In reply to this post by Capece, Tara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** There is (unfortunately) a disparity between how often we do it and how often we should. Power meter readings at the objective. Imaging 0.1 um beads and checking for X, Y, Z registration. Looking at single scan unaveraged images to look for laser stability (reflectance works best for this). We try to look over the shoulders of our unassisted users. This is where the biggest quality control problems are. ========================================================================= Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Office: Skirball 2nd Floor main office, back right http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Capece, Tara Sent: Wednesday, November 09, 2016 8:52 AM To: [hidden email] Subject: Microscope QC Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! Tara Capece University of Rochester ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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In reply to this post by Capece, Tara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Tara, Extensive QC protocols and publications are available from Association of Biomolecular Resource Facilities Light Microscopy Research Group (ABRF LMRG) https://abrf.org/research-group/light-microscopy-research-group-lmrg Many of the authors are on the listserv. Most relevant for you is likely the Cole, Jindasa, Brown 2011 Nature Protocols, Measuring and interpreting point spread functions,to determine confocal microscope resolution and,ensure quality control, available from the LMRG web page, https://abrf.org/sites/default/files/temp/RGs/LMRG/coleetalnatureprotocols2011.pdf George On 11/9/2016 7:52 AM, Capece, Tara wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! > > Tara Capece > University of Rochester -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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In reply to this post by Capece, Tara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, there is an extensive list of quality control measurements in this publication here: http://onlinelibrary.wiley.com/doi/10.1002/jemt.22648/abstract How often you need to do these tests very much depends how much your instruments are used and how well the users are trained (= how careful they are with the instrument). For some instruments it might be necessary to test once per month for others it might be enough to test them every half year. One thing about the laser stability measurement in reflection mode mentioned by Michael Cammer: we have been told by several engineers from different manufacturers to not do this sort of test with any of the newer generation detectors like GaAsP or HyD, they will degrade much faster or you may even damage them if not very careful. With standard PMTs the reflectance test should be fine. Best, Christian On 09.11.2016 14:52, Capece, Tara wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! > > Tara Capece > University of Rochester -- ======================================== Christian Liebig, PhD Light Microscopy Facility Max Planck Institute for Developmental Biology Spemannstraße 35 72076 Tübingen Germany Tel: +497071601443 Fax: +4970716011353 [hidden email] |
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Am 09.11.2016 um 17:24 schrieb Cammer, Michael: > There is (unfortunately) a disparity between how often we do it and how often we should. So very true! Tara, concerning pollen slides, if it is too thin you probably use the wrong species. Hibiscus and Cucurbita are >100 µm, and there is everything in between. This German language pollen wiki page lists species with over 100 µm sized pollen with their botanic name, maybe you spot something you can find in your area: http://pollen.tstebler.ch/MediaWiki/index.php?title=Kategorie:SizeVeryLarge But for testing the microscope we also use beads to record the PSF. Recently we kind of dropped the fluorescent ones an use gold beads instead. It started on the STED for alignment tests, but now we also use them for the regular confocals: no bleaching! It can be quite revealing if you do a time series over 2 hours after switching the instrument on. Any thermal drift won't go unnoticed. Laser stability and intensity tests have already been described by others. In Stephen W. Paddock's "confocal microscopy" isbn 9 781588 293510, is a +50 page chapter by Robert Zucker 'Evaluating Confocal Microscopy System Performance' which I would like to recommend. As a community, we probably should publish our test results on the web, to give others a chance to compare. For example, if you start in this area, it might be difficult to decide whether a chromatic aberration of 100 nm is normal or not. Steffen > > Power meter readings at the objective. > Imaging 0.1 um beads and checking for X, Y, Z registration. > Looking at single scan unaveraged images to look for laser stability (reflectance works best for this). > > We try to look over the shoulders of our unassisted users. This is where the biggest quality control problems are. > > ========================================================================= > Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center > Cell: 914-309-3270 Office: Skirball 2nd Floor main office, back right > http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/ > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Capece, Tara > Sent: Wednesday, November 09, 2016 8:52 AM > To: [hidden email] > Subject: Microscope QC > > > > Does anyone perform quality control on their confocal or multiphoton microscope? If so, how often, on what standards, etc. We use pollen slides every so often, but have noticed some inconsistencies in the last few months. We were hoping to find a better QC protocol that would also account for depth, as the pollen slides are quite thin. Any input or literature is appreciated! > > Tara Capece > University of Rochester > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. > ================================= > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
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