LSM 510 VS LSM 710

[Matiar Jafari]

Hey,

im imaging pyramidal neurons(CA1) and was wondering what are the pros
and cons of using a LSM 710 vs the LSM 510.

just a side note
dont know if this is important but id be imaging at the Nyquist sampling rate


Thank You
--
Matiar Jafari

[Boswell, Carl A - (cboswell)]

Without any intention to single out one individual, I'd like to address a
worsening issue regarding electronic communication.  This is not a diatribe
about "artistic license", the difficulties of English as a second language,
regional differences in slang or syntax or the subtleties of hanging
participles.  What I would like to see is less casual and more appropriate
use of the fundamentals of English by those who should know better.

Professional communications should reflect not only the expertise of the
individual, but their intellectual acuity as well.  We are not Twittering
each other, so we should be writing in complete sentences with proper
capitalization and punctuation.  While I may be a Luddite in this regard,
doesn't it take more effort to purposely write without these attributes than
with them, as all our years of formal training would dictate?

We have enough trouble with the bastardization of the current lexicon.  For
instance, when was the last time you "migrated" a chair from one room to
another, or you asked yourself what "impacted" your decision to buy a
particular car?  In my opinion we should eschew the tendency to accept
trendy but lazy language and work to maintain some semblance discipline, if
only to slow the progression toward the use of "like" three to five times in
every sentence.  (Is that just in America, or has this disheartening trend
spread to other English speaking countries?)

I would propose that a given writing or language style should be appropriate
for the forum in which it is used.  In the case of this forum, where
intelligent and highly educated scientists predominate, we should stick to
that level of discourse.

Rantingly yours,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]

[anuj sharma]

 I truely appreciate...

thats a nice thought and we all should bring it in regular practice.

 

With best wishes

 

Anuj

On Tue, May 19, 2009 at 12:12 AM, Carl Boswell <[hidden email]> wrote:

Without any intention to single out one individual, I'd like to address a worsening issue regarding electronic communication.  This is not a diatribe about "artistic license", the difficulties of English as a second language, regional differences in slang or syntax or the subtleties of hanging participles.  What I would like to see is less casual and more appropriate use of the fundamentals of English by those who should know better.

Professional communications should reflect not only the expertise of the individual, but their intellectual acuity as well.  We are not Twittering each other, so we should be writing in complete sentences with proper capitalization and punctuation.  While I may be a Luddite in this regard, doesn't it take more effort to purposely write without these attributes than with them, as all our years of formal training would dictate?

We have enough trouble with the bastardization of the current lexicon.  For instance, when was the last time you "migrated" a chair from one room to another, or you asked yourself what "impacted" your decision to buy a particular car?  In my opinion we should eschew the tendency to accept trendy but lazy language and work to maintain some semblance discipline, if only to slow the progression toward the use of "like" three to five times in every sentence.  (Is that just in America, or has this disheartening trend spread to other English speaking countries?)

I would propose that a given writing or language style should be appropriate for the forum in which it is used.  In the case of this forum, where intelligent and highly educated scientists predominate, we should stick to that level of discourse.

Rantingly yours,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]

--
Anuj Kumar Sharma
C/o Prof. Uttam Pati,
Lab No-120,
School of Biotechnology,
Jawaharlal Nehru University,
New Delhi-110067 (INDIA)
Mob.- [phone]

[Oshel, Philip Eugene]

Thank you. Nice to know that there is at least one other person
howling from the lonely cliff edge.

Phil

>Without any intention to single out one individual, I'd like to
>address a worsening issue regarding electronic communication.  This
>is not a diatribe about "artistic license", the difficulties of
>English as a second language, regional differences in slang or
>syntax or the subtleties of hanging participles.  What I would like
>to see is less casual and more appropriate use of the fundamentals
>of English by those who should know better.
>
>Professional communications should reflect not only the expertise of
>the individual, but their intellectual acuity as well.  We are not
>Twittering each other, so we should be writing in complete sentences
>with proper capitalization and punctuation.  While I may be a
>Luddite in this regard, doesn't it take more effort to purposely
>write without these attributes than with them, as all our years of
>formal training would dictate?
>
>We have enough trouble with the bastardization of the current
>lexicon.  For instance, when was the last time you "migrated" a
>chair from one room to another, or you asked yourself what
>"impacted" your decision to buy a particular car?  In my opinion we
>should eschew the tendency to accept trendy but lazy language and
>work to maintain some semblance discipline, if only to slow the
>progression toward the use of "like" three to five times in every
>sentence.  (Is that just in America, or has this disheartening trend
>spread to other English speaking countries?)
>
>I would propose that a given writing or language style should be
>appropriate for the forum in which it is used.  In the case of this
>forum, where intelligent and highly educated scientists predominate,
>we should stick to that level of discourse.
>
>Rantingly yours,
>Carl
>
>Carl A. Boswell, Ph.D.
>Molecular and Cellular Biology
>University of Arizona
>520-954-7053
>FAX [phone]

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]

[Dan Focht]

Carl

I agree, It is hard to reinforce proper communication to the younger  
generation with todays social pressures.
Maintaining a good example especially in science and engineering  
can't be understated.
Here is some support from the other side, commerce!

Dan



On May 19, 2009, at 8:33 AM, Philip Oshel wrote:

Thank you. Nice to know that there is at least one other person  
howling from the lonely cliff edge.

Phil

> Without any intention to single out one individual, I'd like to  
> address a worsening issue regarding electronic communication.  This  
> is not a diatribe about "artistic license", the difficulties of  
> English as a second language, regional differences in slang or  
> syntax or the subtleties of hanging participles.  What I would like  
> to see is less casual and more appropriate use of the fundamentals  
> of English by those who should know better.
>
> Professional communications should reflect not only the expertise  
> of the individual, but their intellectual acuity as well.  We are  
> not Twittering each other, so we should be writing in complete  
> sentences with proper capitalization and punctuation.  While I may  
> be a Luddite in this regard, doesn't it take more effort to  
> purposely write without these attributes than with them, as all our  
> years of formal training would dictate?
>
> We have enough trouble with the bastardization of the current  
> lexicon.  For instance, when was the last time you "migrated" a  
> chair from one room to another, or you asked yourself what  
> "impacted" your decision to buy a particular car?  In my opinion we  
> should eschew the tendency to accept trendy but lazy language and  
> work to maintain some semblance discipline, if only to slow the  
> progression toward the use of "like" three to five times in every  
> sentence.  (Is that just in America, or has this disheartening  
> trend spread to other English speaking countries?)
>
> I would propose that a given writing or language style should be  
> appropriate for the forum in which it is used.  In the case of this  
> forum, where intelligent and highly educated scientists  
> predominate, we should stick to that level of discourse.
>
> Rantingly yours,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]



Dan Focht
Bioptechs
3560 Beck Rd.
Butler, PA 16002
V724-282-7145
F724-282-0745
Micro-Environmental Control Systems
www.bioptechs.com

[Ron Anderson-4]

Yes, I saw that and forwarded it to Charlie.

Carl echoes my sentiments exactly

Ron

Philip Oshel wrote:

> Thank you. Nice to know that there is at least one other person
> howling from the lonely cliff edge.
>
> Phil
>
>> Without any intention to single out one individual, I'd like to
>> address a worsening issue regarding electronic communication.  This
>> is not a diatribe about "artistic license", the difficulties of
>> English as a second language, regional differences in slang or syntax
>> or the subtleties of hanging participles.  What I would like to see
>> is less casual and more appropriate use of the fundamentals of
>> English by those who should know better.
>>
>> Professional communications should reflect not only the expertise of
>> the individual, but their intellectual acuity as well.  We are not
>> Twittering each other, so we should be writing in complete sentences
>> with proper capitalization and punctuation.  While I may be a Luddite
>> in this regard, doesn't it take more effort to purposely write
>> without these attributes than with them, as all our years of formal
>> training would dictate?
>>
>> We have enough trouble with the bastardization of the current
>> lexicon.  For instance, when was the last time you "migrated" a chair
>> from one room to another, or you asked yourself what "impacted" your
>> decision to buy a particular car?  In my opinion we should eschew the
>> tendency to accept trendy but lazy language and work to maintain some
>> semblance discipline, if only to slow the progression toward the use
>> of "like" three to five times in every sentence.  (Is that just in
>> America, or has this disheartening trend spread to other English
>> speaking countries?)
>>
>> I would propose that a given writing or language style should be
>> appropriate for the forum in which it is used.  In the case of this
>> forum, where intelligent and highly educated scientists predominate,
>> we should stick to that level of discourse.
>>
>> Rantingly yours,
>> Carl
>>
>> Carl A. Boswell, Ph.D.
>> Molecular and Cellular Biology
>> University of Arizona
>> 520-954-7053
>> FAX [phone]
>

[Christian-103]

I agree that verbal shorthand is a hindrance.  What is the "in jargon" now will not survive five years, and as we've seen just this week, we have valuable references from the 1990's.  "Verbal" short hand cheapens the value of having archives and it makes international communication much more difficult.

I moderate a couple of forum based discussion groups, one with about 5000 members, and I do not tolerate it there, and that only a hobby group, not a collection of professionals.  I always remind people that what they have to say shouldn't be cheapened by laziness.