LZW compressed tiff files

[bluebug]

Hello All,
Can anyone recommend a program to decompress LZW tiff files? I exported the images from Las AF Lite software for downstream processing in another program.

Thanks
C

[Michal Opas]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

C.,
I'd open them in Photoshop then "Save As" TIF compression: none.
I hope this helps.
Michal

On 25/11/2011 8:24 AM, bluebug wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
> Can anyone recommend a program to decompress LZW tiff files? I exported the
> images from Las AF Lite software for downstream processing in another
> program.
>
> Thanks
> C
>
> --
> View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/LZW-compressed-tiff-files-tp7031107p7031107.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>    

--

* Dr. Michal Opas
      Professor
      Department of Laboratory Medicine and Pathobiology
      University of Toronto
      1 King's College Circle
      Medical Sciences Building, room 6326
      Toronto, Ontario, M5S 1A8 Canada

**°°°°°°°°°°°°°**
  phone: [phone] (laboratory)
         [phone] (office)
    fax: [phone]
e*-*mail: **[hidden email] <mailto:[hidden email]>***

*WWW**:** **http://www.utoronto.ca/mocell
*

[bluebug]

Thanks Dr. Opas,
I am currently downloading a trial version of photoshop to save the images without LZW compression.

Thanks for the advice
C

[Mario Emmenlauer]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi,

the freeware and open source libtiff library brings a command-line tool
called "tiffcp", that is intended mainly for this purpose.

 From the website:
tiffcp combines one or more files created according to the Tag Image File
Format, Revision 6.0 into a single TIFF file. Because the output file may be
compressed using a different algorithm than the input files, tiffcp is most
often used to convert between different compression schemes.

http://www.libtiff.org/man/tiffcp.1.html

If you work on Linux, the tool is easily installed and works great for
whole batches of images. On Windows, you would need to look for a
compiled version.

Cheers,

     Mario



On 11/25/2011 04:06 PM, bluebug wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Thanks Dr. Opas,
> I am currently downloading a trial version of photoshop to save the images
> with LZW compression.
>
> Thanks for the advice
> C
>
> --
> View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/LZW-compressed-tiff-files-tp7031107p7031442.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>

[Christoph Gohlke]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Try ImageMagick convert <http://www.imagemagick.org>, which is free,
cross-platform, and scriptable:

     convert -compress None input.tif output.tif

To convert all TIFF files in the current directory on Windows:

     for %1 in (*.tif) do C:\ImageMagick\convert.exe -compress None %1
raw_%1

Christoph


On 11/25/2011 5:24 AM, bluebug wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
> Can anyone recommend a program to decompress LZW tiff files? I exported the
> images from Las AF Lite software for downstream processing in another
> program.
>
> Thanks
> C
>
> --
> View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/LZW-compressed-tiff-files-tp7031107p7031107.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.
>
>

[Nynne Meyn Christensen]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,
We are currently testing the objectives on our new confocal microscope and want to check how the different lasers align with the help of beads (um size range). However, I don't seem to be able to find a bead the 405 laser can excite. Has anyone got any ideas who might make one?

Thanks,
Nynne


Nynne Meyn Christensen
Center Manager

mobil: 30 58 60 91,
email: [hidden email]

Center for Advanced Bioimaging (CAB)
Department of Biology
University of Copenhagen
August Krogh, 6th floor, room 636
Universitetsparken 13
2200 København N

[Pascal Weber]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You should find them here.

http://www.spherotech.com/AlignmentParticles.htm

Regards

[tineke vendrig]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Did you try the beads of molecular probes? T14792

Good luck!

Regards,

Tineke Vendrig

2011/11/28 Nynne Meyn Christensen <[hidden email]>

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
> We are currently testing the objectives on our new confocal microscope and
> want to check how the different lasers align with the help of beads (um
> size range). However, I don't seem to be able to find a bead the 405 laser
> can excite. Has anyone got any ideas who might make one?
>
> Thanks,
> Nynne
>
>
> Nynne Meyn Christensen
> Center Manager
>
> mobil: 30 58 60 91,
> email: [hidden email]
>
> Center for Advanced Bioimaging (CAB)
> Department of Biology
> University of Copenhagen
> August Krogh, 6th floor, room 636
> Universitetsparken 13
> 2200 København N
>

--
Tineke Vendrig, ing
technical engineer optical microscopy
Delft University of Technology
Bionano Science
Kavli Institute of Nanoscience
Lorentzweg 1
2628LJ Delft
room F185
Tel: [phone]
Fax:[phone]
email: [hidden email]
mobile phone: [phone]

[Steffen Dietzel]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,

we are dismantling a Zeiss LSM 410. Nobody has used it in a while and we
need the space. We plan to keep the microscope proper and the table, but
we have to move out everything else.

If someone should be interested in the remains or parts of them, please
contact me off list. We'd be happy to give them away for free, as long
as you pick them up in Munich or arrange for transport.

As I said, the instrument was not used in a while (and never by me), and
rumor has it that at least one of the lasers didn't work any more. Other
than that there are no problems I heard of.

Steffen

------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

Mail room:
Marchioninistr. 15, D-81377 München

Building location:
Marchioninistr. 27,  München-Großhadern