Laser Safety Issues
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:%[hidden email]>> |
 
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Periasamy, Ammasi (ap3t) |
Re: Laser Safety Issues
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If it is a commercial unit the company will follow the FDA laser safety rules. According to the Federal laser safety rules you should display a bright sign board the "laser is on or in use" at the entrance of the room. This sign board is available in the market. If you have Mira like 2p lasers where one can easily open the box for alignment, you should have the protective goggles for that particular wavelength range. There is no need to have a laser protective goggles for the commercial confocal unit, because the laser beam shows up only when yu click the scan button. This procedure is acceptable for our university biosafety/laser safety people. Moreover, I teach in my class on laser safety too. Here is the web site you can see obtain more information on the classification of lasers and laser safety. https://www.osha.gov/dts/osta/otm/otm_iii/otm_iii_6.html It may be a good idea to have a user group meeting to explain the basic laser safety issues, so that the users will not expose their eyes to the laser beam over the objective lens while the scan button is on for live imaging mode. Also, you can list about the basic laser safety on the entrance door of the each confocal room. Hope this helps. Dr. Ammasi Periasamy Professor & Center Director W.M. Keck Center for Cellular Imaging (KCCI) (University of Virginia Imaging Center) Mail or FedEx or UPS: Keck Center for Cellular Imaging University of Virginia Biology, Gilmer Hall, 485 McCormick Rd. Charlottesville, VA 22904, USA Office Location: Physical and Life Sciences Building (PLSB-B005), White Head Rd., Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http://www.kcci.virginia.edu/contact/peri.php ************************ 13th Annual Workshop on FRET & FLIM Microscopy, March 10-14, 2014 http://www.kcci.virginia.edu/workshop/workshop2014/index.php ************************* -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Thursday, October 24, 2013 3:12 PM To: [hidden email] Subject: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:%[hidden email]>> |
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Elberger, Andrea June-2 |
Re: Laser Safety Issues
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** AMMASI - This is helpful. Thanks. ANDREA -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Periasamy, Ammasi (ap3t) Sent: Thursday, October 24, 2013 4:00 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If it is a commercial unit the company will follow the FDA laser safety rules. According to the Federal laser safety rules you should display a bright sign board the "laser is on or in use" at the entrance of the room. This sign board is available in the market. If you have Mira like 2p lasers where one can easily open the box for alignment, you should have the protective goggles for that particular wavelength range. There is no need to have a laser protective goggles for the commercial confocal unit, because the laser beam shows up only when yu click the scan button. This procedure is acceptable for our university biosafety/laser safety people. Moreover, I teach in my class on laser safety too. Here is the web site you can see obtain more information on the classification of lasers and laser safety. https://www.osha.gov/dts/osta/otm/otm_iii/otm_iii_6.html It may be a good idea to have a user group meeting to explain the basic laser safety issues, so that the users will not expose their eyes to the laser beam over the objective lens while the scan button is on for live imaging mode. Also, you can list about the basic laser safety on the entrance door of the each confocal room. Hope this helps. Dr. Ammasi Periasamy Professor & Center Director W.M. Keck Center for Cellular Imaging (KCCI) (University of Virginia Imaging Center) Mail or FedEx or UPS: Keck Center for Cellular Imaging University of Virginia Biology, Gilmer Hall, 485 McCormick Rd. Charlottesville, VA 22904, USA Office Location: Physical and Life Sciences Building (PLSB-B005), White Head Rd., Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http://www.kcci.virginia.edu/contact/peri.php ************************ 13th Annual Workshop on FRET & FLIM Microscopy, March 10-14, 2014 http://www.kcci.virginia.edu/workshop/workshop2014/index.php ************************* -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Thursday, October 24, 2013 3:12 PM To: [hidden email] Subject: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:%[hidden email]>> |
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In reply to this post by Elberger, Andrea June-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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In reply to this post by Elberger, Andrea June-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, 1. "Do not look at laser(s) with remaining eye." 2. light tight box, laser safety interlock. 3. No. Murphy's Law dictates they would remove the eyeware before #1. 4. No. The really good service people share the measurements they make at the laser output and/or back focal plane. You're welcome and stay safe, George On 10/24/2013 2:12 PM, Elberger, Andrea June wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > TO THE CONFOCAL LIST MEMBERS: > > I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. > I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. > For example: > > 1) What training requirements/procedures do you have for trainers and end-users? > > 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? > > 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? > > 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? > Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. > > Thanks for your time. > > ANDREA > > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility > The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]<mailto:%[hidden email]>> > > |
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Elberger, Andrea June-2 |
Re: Laser Safety Issues
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In reply to this post by Neeraj Gohad-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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Indig, Fred (NIH/NIA/IRP) [E] |
Re: Laser Safety Issues
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I haven't read anything specific to confocal lasers, but a quick search revealed several papers documenting retinal damage due to class 3b lasers, such as http://link.springer.com/article/10.1007%2Fs10384-011-0031-5 Judging from the literature, 3a lasers seem to cause recoverable blindness even with relatively long exposure times (several seconds), while 3b lasers can cause long-term retinal damage even with short exposure times (under one second). I'm guessing there wouldn't be very many confocal microscopy specific eye injuries, because users who have access to such equipment also tend to be well aware of the potential hazards of laser light. Benjamin E. Smith, Ph.D. Samuel Roberts Noble Microscopy Laboratory Research Scientist II University of Oklahoma Norman, OK 73019 E-mail: [hidden email] Voice 405-325-4391 FAX 405-325-7619 http://www.microscopy.ou.edu/ ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Indig, Fred (NIH/NIA/IRP) [E] [[hidden email]] Sent: Friday, October 25, 2013 2:37 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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In reply to this post by Indig, Fred (NIH/NIA/IRP) [E]
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Not with confocal, but yes with multiphoton with an open beam path. Wavelengths somewhere in the range of 820 to 960 nm. Both an injury and black spots on the wall. According to a quick google search, commercial CMOS cameras are susceptible to laser damage too. =========================================================================== Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Lab: 212-263-3208 http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Indig, Fred (NIH/NIA/IRP) [E] Sent: Friday, October 25, 2013 3:37 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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Elberger, Andrea June-2 |
Re: Laser Safety Issues
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In reply to this post by Neeraj Gohad-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the heads up on the LIA. I did not know of their existence, nor that they had training courses. So this is very useful. Best wishes, ANDREA -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Friday, October 25, 2013 2:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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Elberger, Andrea June-2 |
Re: Laser Safety Issues
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In reply to this post by Indig, Fred (NIH/NIA/IRP) [E]
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** FRED - We have had no injuries on our campus. Our first confocal LSM was operation in 1993. ANDREA ELBERGER Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Indig, Fred (NIH/NIA/IRP) [E] Sent: Friday, October 25, 2013 2:37 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Eye injury or skin burn? Craig On Fri, Oct 25, 2013 at 2:30 PM, Cammer, Michael <[hidden email] > wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Not with confocal, but yes with multiphoton with an open beam path. > Wavelengths somewhere in the range of 820 to 960 nm. Both an injury and > black spots on the wall. > > According to a quick google search, commercial CMOS cameras are > susceptible to laser damage too. > > =========================================================================== > Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU > Langone Medical Center > Cell: 914-309-3270 Lab: 212-263-3208 > http://ocs.med.nyu.edu/microscopy & > http://www.med.nyu.edu/skirball-lab/dustinlab/ > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Indig, Fred (NIH/NIA/IRP) [E] > Sent: Friday, October 25, 2013 3:37 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Just out of curiosity, is anyone aware of an accident or injury involving > the laser of a confocal LSM? > > > Fred E. Indig, Ph.D. > Head, Confocal Imaging Facility > > Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH > 251 Bayview Bld. > Baltimore, MD 21224-6825 > > Tel. 410-558-8173 > Fax 410-558-8236 > [hidden email] > ________________________________________ > From: Neeraj Gohad [[hidden email]] > Sent: Friday, October 25, 2013 3:23 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > You are welcome Andrea, it's a good resource and our laser safety officer > was quite happy that I followed the recommendations in it. If there are > extensive custom setups, our Univ. also has access to Laser Safety in > Educational Institutions Training -online course from LIA. > > Best Regards, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological > Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > http://www.clemson.edu/sigmaxi/index.html > > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Elberger, Andrea June > Sent: Friday, October 25, 2013 3:13 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > NEERAJ - Thanks for the link to the ANSI site, it will be a useful > reference point for us. > > ANDREA > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of > Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]> > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Neeraj Gohad > Sent: Thursday, October 24, 2013 6:57 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Andrea, > > Might also want to check out the link below. The ANSI standard has some > good pointers as well. > > https://www.lia.org/store/ANSI/114 > > ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) > > Best, > > Neeraj. > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological > Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > > Sent from my iPhone > > On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email] > <mailto:[hidden email]>> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > TO THE CONFOCAL LIST MEMBERS: > > I am a member of a campus-wide Laser Safety Subcommittee, and we are > trying to establish policies and standards for numerous systems (more than > microscopes) that use lasers for research. In the case of Confocal Laser > Scanning Microscope systems, all those on our campus are commercially > available systems. > I am seeking information on what CLSM facilities have done, or tried to > do, related to laser safety policies and issues. > For example: > > 1) What training requirements/procedures do you have for trainers and > end-users? > > 2) Do you have any modifications to your existing commercial systems, > e.g. a screen around the microscope or stage where the laser beam is > visible? > > 3) Do you require users to wear protective eyewear at any time during > operation of the CLSM? > > 4) Do you have the manufacturer's service people carry out any > measurements that would be used in laser safety calculations? > Any information on other procedures or issues related to use of the lasers > in CLSMs would be greatly appreciated. > > Thanks for your time. > > ANDREA > > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of > Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]<mailto:[hidden email]><mailto:% > [hidden email]<mailto:[hidden email]>>> > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check > this email and any attachments for the presence of viruses. The > organization accepts no liability for any damage caused by any virus > transmitted by this email. > ================================= > |
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*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Eye injury. =========================================================================== Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Lab: 212-263-3208 http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau Sent: Friday, October 25, 2013 5:14 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Eye injury or skin burn? Craig On Fri, Oct 25, 2013 at 2:30 PM, Cammer, Michael <[hidden email] > wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Not with confocal, but yes with multiphoton with an open beam path. > Wavelengths somewhere in the range of 820 to 960 nm. Both an injury > and black spots on the wall. > > According to a quick google search, commercial CMOS cameras are > susceptible to laser damage too. > > ====================================================================== > ===== Michael Cammer, Microscopy Core & Dustin Lab , Skirball > Institute, NYU Langone Medical Center > Cell: 914-309-3270 Lab: 212-263-3208 > http://ocs.med.nyu.edu/microscopy & > http://www.med.nyu.edu/skirball-lab/dustinlab/ > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] > On Behalf Of Indig, Fred (NIH/NIA/IRP) [E] > Sent: Friday, October 25, 2013 3:37 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Just out of curiosity, is anyone aware of an accident or injury > involving the laser of a confocal LSM? > > > Fred E. Indig, Ph.D. > Head, Confocal Imaging Facility > > Biomedical Research Center (BRC) Rm 8B135 National Institute on > Aging/NIH > 251 Bayview Bld. > Baltimore, MD 21224-6825 > > Tel. 410-558-8173 > Fax 410-558-8236 > [hidden email] > ________________________________________ > From: Neeraj Gohad [[hidden email]] > Sent: Friday, October 25, 2013 3:23 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > You are welcome Andrea, it's a good resource and our laser safety > officer was quite happy that I followed the recommendations in it. If > there are extensive custom setups, our Univ. also has access to Laser > Safety in Educational Institutions Training -online course from LIA. > > Best Regards, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of > Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n. > html > > http://www.clemson.edu/sigmaxi/index.html > > > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] > On Behalf Of Elberger, Andrea June > Sent: Friday, October 25, 2013 3:13 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > NEERAJ - Thanks for the link to the ANSI site, it will be a useful > reference point for us. > > ANDREA > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University > of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]> > > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] > On Behalf Of Neeraj Gohad > Sent: Thursday, October 24, 2013 6:57 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Andrea, > > Might also want to check out the link below. The ANSI standard has > some good pointers as well. > > https://www.lia.org/store/ANSI/114 > > ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) > > Best, > > Neeraj. > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of > Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n. > html > > > Sent from my iPhone > > On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" > <[hidden email] <mailto:[hidden email]>> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > TO THE CONFOCAL LIST MEMBERS: > > I am a member of a campus-wide Laser Safety Subcommittee, and we are > trying to establish policies and standards for numerous systems (more > than > microscopes) that use lasers for research. In the case of Confocal > Laser Scanning Microscope systems, all those on our campus are > commercially available systems. > I am seeking information on what CLSM facilities have done, or tried > to do, related to laser safety policies and issues. > For example: > > 1) What training requirements/procedures do you have for trainers and > end-users? > > 2) Do you have any modifications to your existing commercial systems, > e.g. a screen around the microscope or stage where the laser beam is > visible? > > 3) Do you require users to wear protective eyewear at any time during > operation of the CLSM? > > 4) Do you have the manufacturer's service people carry out any > measurements that would be used in laser safety calculations? > Any information on other procedures or issues related to use of the > lasers in CLSMs would be greatly appreciated. > > Thanks for your time. > > ANDREA > > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University > of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]<mailto:[hidden email]><mailto:% > [hidden email]<mailto:[hidden email]>>> > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of > the intended recipient(s) and may contain information that is > proprietary, confidential, and exempt from disclosure under applicable > law. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you have received this email in error please notify the > sender by return email and delete the original message. Please note, > the recipient should check this email and any attachments for the > presence of viruses. The organization accepts no liability for any > damage caused by any virus transmitted by this email. > ================================= > ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** With respect to, "According to a quick google search, commercial CMOS cameras are susceptible to laser damage too." Do not look at laser with remaining pixels. On 10/25/2013 3:30 PM, Cammer, Michael wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Not with confocal, but yes with multiphoton with an open beam path. Wavelengths somewhere in the range of 820 to 960 nm. Both an injury and black spots on the wall. > > According to a quick google search, commercial CMOS cameras are susceptible to laser damage too. > > =========================================================================== > Michael Cammer, Microscopy Core& Dustin Lab , Skirball Institute, NYU Langone Medical Center > Cell: 914-309-3270 Lab: 212-263-3208 > http://ocs.med.nyu.edu/microscopy& http://www.med.nyu.edu/skirball-lab/dustinlab/ > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Indig, Fred (NIH/NIA/IRP) [E] > Sent: Friday, October 25, 2013 3:37 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? > > > Fred E. Indig, Ph.D. > Head, Confocal Imaging Facility > > Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH > 251 Bayview Bld. > Baltimore, MD 21224-6825 > > Tel. 410-558-8173 > Fax 410-558-8236 > [hidden email] > ________________________________________ > From: Neeraj Gohad [[hidden email]] > Sent: Friday, October 25, 2013 3:23 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. > > Best Regards, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > http://www.clemson.edu/sigmaxi/index.html > > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June > Sent: Friday, October 25, 2013 3:13 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. > > ANDREA > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]> > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad > Sent: Thursday, October 24, 2013 6:57 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Andrea, > > Might also want to check out the link below. The ANSI standard has some good pointers as well. > > https://www.lia.org/store/ANSI/114 > > ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) > > Best, > > Neeraj. > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > > Sent from my iPhone > > On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June"<[hidden email]<mailto:[hidden email]>> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > TO THE CONFOCAL LIST MEMBERS: > > I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. > I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. > For example: > > 1) What training requirements/procedures do you have for trainers and end-users? > > 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? > > 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? > > 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? > Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. > > Thanks for your time. > > ANDREA > > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. > ================================= > > -- George McNamara, Ph.D. Single Cells Analyst L.J.N. Cooper Lab University of Texas M.D. Anderson Cancer Center Houston, TX 77054 http://works.bepress.com/gmcnamara/26/ |
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In reply to this post by Smith, Benjamin E.
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Here is a link to DoE compiled laser safety report on documented injuries due to lasers. http://www.jlab.org/accel/inj_group/safety/DOE-LSOP/DOElaseraccidentsreport.pdf Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Smith, Benjamin E. Sent: Friday, October 25, 2013 4:10 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I haven't read anything specific to confocal lasers, but a quick search revealed several papers documenting retinal damage due to class 3b lasers, such as http://link.springer.com/article/10.1007%2Fs10384-011-0031-5 Judging from the literature, 3a lasers seem to cause recoverable blindness even with relatively long exposure times (several seconds), while 3b lasers can cause long-term retinal damage even with short exposure times (under one second). I'm guessing there wouldn't be very many confocal microscopy specific eye injuries, because users who have access to such equipment also tend to be well aware of the potential hazards of laser light. Benjamin E. Smith, Ph.D. Samuel Roberts Noble Microscopy Laboratory Research Scientist II University of Oklahoma Norman, OK 73019 E-mail: [hidden email] Voice 405-325-4391 FAX 405-325-7619 http://www.microscopy.ou.edu/ ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Indig, Fred (NIH/NIA/IRP) [E] [[hidden email]] Sent: Friday, October 25, 2013 2:37 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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Elberger, Andrea June-2 |
Re: Laser Safety Issues
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks very much. This will help us to focus in on where our problem areas might be. Best wishes, ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Monday, October 28, 2013 10:48 AM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Here is a link to DoE compiled laser safety report on documented injuries due to lasers. http://www.jlab.org/accel/inj_group/safety/DOE-LSOP/DOElaseraccidentsreport.pdf Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Smith, Benjamin E. Sent: Friday, October 25, 2013 4:10 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I haven't read anything specific to confocal lasers, but a quick search revealed several papers documenting retinal damage due to class 3b lasers, such as http://link.springer.com/article/10.1007%2Fs10384-011-0031-5 Judging from the literature, 3a lasers seem to cause recoverable blindness even with relatively long exposure times (several seconds), while 3b lasers can cause long-term retinal damage even with short exposure times (under one second). I'm guessing there wouldn't be very many confocal microscopy specific eye injuries, because users who have access to such equipment also tend to be well aware of the potential hazards of laser light. Benjamin E. Smith, Ph.D. Samuel Roberts Noble Microscopy Laboratory Research Scientist II University of Oklahoma Norman, OK 73019 E-mail: [hidden email] Voice 405-325-4391 FAX 405-325-7619 http://www.microscopy.ou.edu/ ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Indig, Fred (NIH/NIA/IRP) [E] [[hidden email]] Sent: Friday, October 25, 2013 2:37 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? Fred E. Indig, Ph.D. Head, Confocal Imaging Facility Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH 251 Bayview Bld. Baltimore, MD 21224-6825 Tel. 410-558-8173 Fax 410-558-8236 [hidden email] ________________________________________ From: Neeraj Gohad [[hidden email]] Sent: Friday, October 25, 2013 3:23 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. Best Regards, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html http://www.clemson.edu/sigmaxi/index.html -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June Sent: Friday, October 25, 2013 3:13 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]> -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad Sent: Thursday, October 24, 2013 6:57 PM To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Andrea, Might also want to check out the link below. The ANSI standard has some good pointers as well. https://www.lia.org/store/ANSI/114 ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) Best, Neeraj. Neeraj V. Gohad, Ph.D. Assistant Research Professor Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435 http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html Sent from my iPhone On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** TO THE CONFOCAL LIST MEMBERS: I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. For example: 1) What training requirements/procedures do you have for trainers and end-users? 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. Thanks for your time. ANDREA Andrea J. Elberger, Ph. D. Professor, Anatomy and Neurobiology Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center 855 Monroe Avenue Memphis, TN 38163 U.S.A. tel: 901-448-4101 FAX: 901-448-7193 <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** See also the Photonics Spectra article at http://photonics.com/Article.aspx?AID=55023 Laser safety: A university perspective Nearly every genre of scientific research has a niche where the laser is used. And, much as radioactive materials have transitioned from physics to biology, laser research techniques are invading arenas of science not traditionally seen as "laser territory." In addition to broader research applications, the increases in quality, variety, flexibility, efficiency, tuning and power have all improved, while costs have come down. These changes have drastically reshaped the use of lasers at research universities. ... The PI must be aware of how work is done in the lab and cannot assume that his or her initial instructions are being carried out safely. The PI should periodically verify that the lab hazards are adequately controlled. Young research students attending graduate school require more guidance and greater oversight to ensure safe work conditions. The liability to the school and PI for a student injury is much greater than for a similar incident with a seasoned research scientist. ... In a case of injury or fatality, as with the accidental death of Sheri Sangji in a university chemistry lab, the university and the PI could be held criminally liable for lack of oversight in the operations of their labs. ... The lesson here is that all lab personnel, beginning with the PI, must supply the up-front effort to implement safety in research processes. ... As the PI incorporates laser safety into the research lab, the LSO must respond with professional guidance and prompt assistance. If the LSO is not able to perform these functions properly, the PI will avoid working with the LSO and implement his own controls, which may be insufficient. Management can assist the safety process by providing the LSO with resources to respond to the PI's needs effectively. /R. DeWayne Holcomb, CHP, CLSO/ /University of Texas at Austin/ // / ** I disagree with the author's statement: / /"/The liability to the school and PI for a student injury is much greater than for a similar incident with a seasoned research scientist./"/ // /and not just because I am a seasoned (possibly overcooked) research scientist. / I do encourage everyone on the listserv to read the entire article (go to the web page). Also keep in mind that even if your lab or core is perfect, there is no reason to think any of your colleagues are safe - whether lasers, chemicals, or biologicals. best wishes, George On 10/28/2013 11:02 AM, Elberger, Andrea June wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > NEERAJ - Thanks very much. This will help us to focus in on where our problem areas might be. > > Best wishes, > > ANDREA > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility > The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]> > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad > Sent: Monday, October 28, 2013 10:48 AM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Here is a link to DoE compiled laser safety report on documented injuries due to lasers. > > http://www.jlab.org/accel/inj_group/safety/DOE-LSOP/DOElaseraccidentsreport.pdf > > Best, > > Neeraj. > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > http://www.clemson.edu/sigmaxi/index.html > > > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Smith, Benjamin E. > Sent: Friday, October 25, 2013 4:10 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I haven't read anything specific to confocal lasers, but a quick search revealed several papers documenting retinal damage due to class 3b lasers, such as http://link.springer.com/article/10.1007%2Fs10384-011-0031-5 > > Judging from the literature, 3a lasers seem to cause recoverable blindness even with relatively long exposure times (several seconds), while 3b lasers can cause long-term retinal damage even with short exposure times (under one second). > > I'm guessing there wouldn't be very many confocal microscopy specific eye injuries, because users who have access to such equipment also tend to be well aware of the potential hazards of laser light. > > Benjamin E. Smith, Ph.D. > Samuel Roberts Noble Microscopy Laboratory Research Scientist II University of Oklahoma Norman, OK 73019 > E-mail: [hidden email] > Voice 405-325-4391 > FAX 405-325-7619 > http://www.microscopy.ou.edu/ > ________________________________________ > From: Confocal Microscopy List [[hidden email]] on behalf of Indig, Fred (NIH/NIA/IRP) [E] [[hidden email]] > Sent: Friday, October 25, 2013 2:37 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM? > > > Fred E. Indig, Ph.D. > Head, Confocal Imaging Facility > > Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH > 251 Bayview Bld. > Baltimore, MD 21224-6825 > > Tel. 410-558-8173 > Fax 410-558-8236 > [hidden email] > ________________________________________ > From: Neeraj Gohad [[hidden email]] > Sent: Friday, October 25, 2013 3:23 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA. > > Best Regards, > > Neeraj. > > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > http://www.clemson.edu/sigmaxi/index.html > > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elberger, Andrea June > Sent: Friday, October 25, 2013 3:13 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us. > > ANDREA > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]> > > > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neeraj Gohad > Sent: Thursday, October 24, 2013 6:57 PM > To: [hidden email] > Subject: Re: Laser Safety Issues > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Andrea, > > Might also want to check out the link below. The ANSI standard has some good pointers as well. > > https://www.lia.org/store/ANSI/114 > > ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009) > > Best, > > Neeraj. > > Neeraj V. Gohad, Ph.D. > Assistant Research Professor > Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences > 132 Long Hall > Clemson University > Clemson,SC-29634 > Phone: 864-656-3597 > Fax: 864-656-0435 > > http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html > > > Sent from my iPhone > > On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June"<[hidden email]<mailto:[hidden email]>> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > TO THE CONFOCAL LIST MEMBERS: > > I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems. > I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues. > For example: > > 1) What training requirements/procedures do you have for trainers and end-users? > > 2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible? > > 3) Do you require users to wear protective eyewear at any time during operation of the CLSM? > > 4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations? > Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated. > > Thanks for your time. > > ANDREA > > > > Andrea J. Elberger, Ph. D. > Professor, Anatomy and Neurobiology > Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center > 855 Monroe Avenue > Memphis, TN 38163 U.S.A. > tel: 901-448-4101 > FAX: 901-448-7193 > <mailto: [hidden email]<mailto:[hidden email]><mailto:%[hidden email]<mailto:[hidden email]>>> > > -- George McNamara, Ph.D. Single Cells Analyst L.J.N. Cooper Lab University of Texas M.D. Anderson Cancer Center Houston, TX 77054 Tattletales http://works.bepress.com/gmcnamara/26/ |
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In reply to this post by Elberger, Andrea June-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Since we are on the confocal list, let me ask the following: How much danger is there really from lasers in commercial instruments, provided the housing is intact. Let's put aside for a moment those devices with an open laser beam on the bench - that's obviously a different pair of shoes. In a typical commercial confocal, the laser comes out only after running through lenses, so whatever comes out of the objective (or out of the revolver, should the objective be screwed out) is not a parallel beam but a cone of light which increases in diameter rather fast. So I would think that unless someone manages to put his eye directly in front of a long distance, low NA objective, the whole thing would be probably less dangerous than a laser pointer held by the stereotypical distraught professor in front of his audience. Even if the confocal laser has >100 mW to start with, as in some FRAP-Designs. Did I miss something? Not that I would recommend messing around with the laser light, but so far I always thought this danger to be negligible. Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Steffen, I agree, in normal use the beam from the objective is divergent enough that it doesn't have most of the risks traditionally associated with laser systems. What makes lasers hazardous, from a Health & Safety perspective, is the parallel beam, and its potential of doing significant damage even with a reflection of a few percent at a distance of meters. Not that I recommend carelessness. But what always strike me in this debate is that Health & Safety departments tend to be extremely worried about lasers, but are much less concerned about lamps; while in the critical area at the top of the objective a 100W+ mercury or metal-halide fluorescence excitation lamp is probably as dangerous as a laser system. And e.g. the "hot" end of a liquid fiber, for liquid-fiber coupled lamp systems, is something you should be very careful with. I think it is a good idea to give users some basic safety training for microscopes regardless of whether they contain lasers or not - don't place your eyes directly above the objective lens, be careful with UV, beware of using cracked or delaminated filters, etc. From the safety perspective, what matters for normal users is the safety rating of the assembled system. A system can contain Class 3 lasers and be rated Class 1. But if you have your own maintenance people working on microscopes, who are probably dissassembling it and overriding safety interlocks, that brings with it other (and significant) responsibilities. Emmanuel -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steffen Dietzel Sent: Tuesday, 29 October, 2013 11:28 To: [hidden email] Subject: Re: Laser Safety Issues ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Since we are on the confocal list, let me ask the following: How much danger is there really from lasers in commercial instruments, provided the housing is intact. Let's put aside for a moment those devices with an open laser beam on the bench - that's obviously a different pair of shoes. In a typical commercial confocal, the laser comes out only after running through lenses, so whatever comes out of the objective (or out of the revolver, should the objective be screwed out) is not a parallel beam but a cone of light which increases in diameter rather fast. So I would think that unless someone manages to put his eye directly in front of a long distance, low NA objective, the whole thing would be probably less dangerous than a laser pointer held by the stereotypical distraught professor in front of his audience. Even if the confocal laser has >100 mW to start with, as in some FRAP-Designs. Did I miss something? Not that I would recommend messing around with the laser light, but so far I always thought this danger to be negligible. Steffen -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Walter-Brendel-Zentrum für experimentelle Medizin (WBex) Head of light microscopy Mail room: Marchioninistr. 15, D-81377 München Building location: Marchioninistr. 27, München-Großhadern |
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