Lasermicrodissection comparison

[Ralf Palmisano]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear community,

It is slightly off-topic (only just). I am currently trying to get an
overview for a l laser microdissection system.
As no around here as any experience with the different approaches by
commercial suppliers (Leica, Zeiss, Nikon, ArcturusXT e.g.) I would be
more than grateful if some of you are willing to share the pro and con
of the various techniques (gravity dependent or laser burst etc.) as
well as your experience with a given system. Also input of the
utilisation in a heavy multi-user environment would be appreciated.

Looking forward to your comments!

Cheers Ralph

--
Ralph Palmisano
Head - Optical Imaging Centre Erlangen

Fellow Royal Microscopical Society
Member Royal Society of Medicine

Speaker Scientific Advisory Board "German Society for Microscopy and Image Analysis"
Board of Directors "Core Technologies for Life Sciences"

Cauerstr. 3
91058 Erlangen, Germany

[phone] (Office)
[phone] (Secretary)

www.oice.uni-erlangen.de

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Ralph

We have an MMI microdissection system. Picking the tissue works with charged silicon in the Eppendorf tube cap which comes in contact with a membrane of the opposite charge. The sample is on the other side of the membrane so it is lifted with the membrane. That can be done in RNAse-free conditions for RNA sequencing.

It works very well and the best is that we never loose any tissue, regardless of whether we cut a tiny or a large area. As I understood it this is one of the disadvantages of the catapult system but I have never tried.

However the software is not always stable. Our system is a bit special because it has 2 cameras (1 B/W and 1 colour) but it is not rare that suddenly the software doesn’t recognize the B/W camera. We have found tricks around this though.

As a whole we are happy with it. We also use it as a slide scanner and a cell picker.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Ralf Palmisano OICE
Sent: 30 October 2019 11:47
To: [hidden email]
Subject: Lasermicrodissection comparison

*****
To join, leave or search the confocal microscopy listserv, go to:
https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C29f3417940294c443b3d08d75d271b61%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637080294867703161&amp;sdata=z91%2B8lZmklYsSeEeKXOxzFufqmCKlDvoVYAd0epsGC0%3D&amp;reserved=0
Post images on https://eur01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C29f3417940294c443b3d08d75d271b61%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637080294867703161&amp;sdata=WUBeKrMBVuq2ZpjMEQLpJU4njZLZRofgGJEqYcuwhTI%3D&amp;reserved=0 and include the link in your posting.
*****

Dear community,

It is slightly off-topic (only just). I am currently trying to get an overview for a l laser microdissection system.
As no around here as any experience with the different approaches by commercial suppliers (Leica, Zeiss, Nikon, ArcturusXT e.g.) I would be more than grateful if some of you are willing to share the pro and con of the various techniques (gravity dependent or laser burst etc.) as well as your experience with a given system. Also input of the utilisation in a heavy multi-user environment would be appreciated.

Looking forward to your comments!

Cheers Ralph

--
Ralph Palmisano
Head - Optical Imaging Centre Erlangen

Fellow Royal Microscopical Society
Member Royal Society of Medicine

Speaker Scientific Advisory Board "German Society for Microscopy and Image Analysis"
Board of Directors "Core Technologies for Life Sciences"

Cauerstr. 3
91058 Erlangen, Germany

[phone] (Office)
[phone] (Secretary)

https://eur01.safelinks.protection.outlook.com/?url=www.oice.uni-erlangen.de&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C29f3417940294c443b3d08d75d271b61%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637080294867703161&amp;sdata=QUOgSmT1bT5iEuO5ZxSwsKg%2BV5YT1MU%2BqvWGIrGYt3U%3D&amp;reserved=0


När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.