Leica: 160x GSD objective in a conventional confocal ?

[Renato A. Mortara]

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Thanks Pedro, and yes, Leica has been rather slow in helping with this issue

Renato

-----Mensagem original-----
De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Pedro Almada
Enviada em: quarta-feira, 12 de novembro de 2014 11:50
Para: [hidden email]
Assunto: Re: 160x GSD objective in a conventional confocal ?

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The problem with Leica is they do some of the corrections outside of the objective, on the tube lens. Not sure if the GSD has a specific tube lens matching that objective as Leica is never forward with these things...

On 12 November 2014 10:51, Renato Mortara <[hidden email]> wrote:

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> Dear all
>
>
>
> Our institution has just acquired a GSD system from Leica that is
> based on a 160x 1.43 objective that sits in a ‘heavy’ stage they named
> ‘Sumo’. I have tried to approach Leica to test this objective in a
> conventional SP5 microscope but the only unsatisfying answer I got
> from Leica was ‘the GSD system is manufactured in a different unit
> from the confocals…’ and the GSD people are, understandably, reluctant
> to let me remove it and try on our confocal.
>
>
>
> I had previous excellent experience with a 150x TIRF on a spinning
> disk system. Has anyone tried the 160x on a confocal microscope? Any inputs?
>
>
>
> Thanks !
>
>
> Renato
>
>
>
> Dr. Renato Arruda Mortara
>
> Disciplina de Parasitologia
>
> Escola Paulista de Medicina - UNIFESP
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