Leica DFC500 update to Windows7
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Paloma Domínguez Giménez |
Leica DFC500 update to Windows7
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, I write you searching for some advice. Because I am experiencing some troubles updating a Leica DFC500 camera to windows7. The fact is that I updated the drivers and firmware successfully, but when connecting the camera to the windows 7 PC, it is recognized as a device but it not operative. Even if I choose the Leica firewire driver (updated version too), and I see the camera connected to the system, still LAS does not recognize any camera connected at all. On top of that, in the same computer I have installed LAS AF 2.7 that runs with a DFC350FX camera, and both were working nicely so far at windows 7. However when I finish installing the DFC500 and LAS, then the DFC350 driver disappears and LAS AF does not work anymore. Do you know if there might be any incompatibility between DFC500 and DFC350 in Windows7? Can they work together in the same computer? In Windows XP there was no problem at all..... I suppose that many of you have done this before, so I wonder if any of you experienced the same problems. Any advice will be more than welcome. Thanks in advanced for your help anyway! Paloma Dominguez Gimenez, PhD Microscopy Facility Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER) Avda. Américo Vespucio s/n Seville 41092 (SPAIN) Tlf. 34-954 468223 E-mail: [hidden email]<mailto:[hidden email]> Web: http://www.cabimer.es/web/es/unidades-apoyo/microscopia Red Española de Microscopía Óptica Avanzada (REMOA) http://remoa.wikispaces.com<http://remoa.wikispaces.com/> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Paloma, If I remember right, you have to update the camera firmware from an XP system. Jens --------------------------------------------------------------- Dr. Jens Bernhard Bosse Heinrich Pette Institute Leibniz Institute for Experimental Virology Structural Biology of Viruses Subunit Quantitative Virology Martinistr. 52 20251 Hamburg Germany email: [hidden email] phone: +49-040-48051-273 URL: http://www.hpi-hamburg.de/en/research-teams/research-units/structural-biology-of-viruses/subunit-quantitative-virology/ This electronic communication, including any attached documents, may contain confidential and/or legally privileged information that is intended only for use by the recipient(s) named above. If you have received this communication in error, please notify the sender immediately and delete the communication and any attachments. > On 15 Jul 2016, at 09:36, Paloma Domínguez Giménez <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear all, > > I write you searching for some advice. Because I am experiencing some troubles updating a Leica DFC500 camera to windows7. > The fact is that I updated the drivers and firmware successfully, but when connecting the camera to the windows 7 PC, it is recognized as a device but it not operative. > Even if I choose the Leica firewire driver (updated version too), and I see the camera connected to the system, still LAS does not recognize any camera connected at all. > On top of that, in the same computer I have installed LAS AF 2.7 that runs with a DFC350FX camera, and both were working nicely so far at windows 7. However when I finish installing the DFC500 and LAS, then the DFC350 driver disappears and LAS AF does not work anymore. > Do you know if there might be any incompatibility between DFC500 and DFC350 in Windows7? Can they work together in the same computer? In Windows XP there was no problem at all..... > I suppose that many of you have done this before, so I wonder if any of you experienced the same problems. > Any advice will be more than welcome. > Thanks in advanced for your help anyway! > > Paloma Dominguez Gimenez, PhD > Microscopy Facility > Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER) > Avda. Américo Vespucio s/n > Seville 41092 (SPAIN) > Tlf. 34-954 468223 > E-mail: [hidden email]<mailto:[hidden email]> > Web: http://www.cabimer.es/web/es/unidades-apoyo/microscopia > > Red Española de Microscopía Óptica Avanzada (REMOA) > http://remoa.wikispaces.com<http://remoa.wikispaces.com/> |
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0000001ed7f52e4a-dmarc-request |
objective catches bubbles during tissue imaging
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, we have a strange problem with a Zeiss 20x NA 1.0 objective catching oxygen bubbles during live tissue imaging. We were doing these experiments sucessfully since many years on an Olympus setup, purging the medium with oxygen/5%CO2 close to the tissue (extracted lymph node) which is glued onto a 30 mm dish and kept at 37 degrees. The problem seems to be that the little oxygen bubbles are caught by the concave front lens of the Zeiss objective, unsurpringly resulting in a dramatic loss of signal. The Olympus 25x NA 1.05 lens did have a larger, flat front lens and we did not have this problem. I tried bubbling the oxygen into the bottle with the medium which is a bit further away, but was not able to keep the tissue alive during these conditions, the cells stop moving. There are several of these objectives from Zeiss and our Zeiss rep could not tell me if there are some with flat front lenses, we have an older 421452-9800-000. Has anyone experienced similar problems and solved the issue? On another note, is the CO2 in the Oxygen/CO2 mixture essential for these experiments (tracking and interaction of lymphocytes in lymph nodes)? best wishes Andreas |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Andreas Is this an upright or inverted scope? You said the specimen is on a dish but is it in a closed or open type system? Are you dealing with condensation also? Would you send me a picture of your setup? and/or You can call Bioptechs and ask for Dan. Dan > On Jul 15, 2016, at 4:22 AM, [hidden email] wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Dear all, > > we have a strange problem with a Zeiss 20x NA 1.0 objective catching oxygen bubbles during live tissue imaging. We were doing these experiments sucessfully since many years on an Olympus setup, purging the medium with oxygen/5%CO2 close to the tissue (extracted lymph node) which is glued onto a 30 mm dish and kept at 37 degrees. The problem seems to be that the little oxygen bubbles are caught by the concave front lens of the Zeiss objective, unsurpringly resulting in a dramatic loss of signal. The Olympus 25x NA 1.05 lens did have a larger, flat front lens and we did not have this problem. I tried bubbling the oxygen into the bottle with the medium which is a bit further away, but was not able to keep the tissue alive during these conditions, the cells stop moving. There are several of these objectives from Zeiss and our Zeiss rep could not tell me if there are some with flat front lenses, we have an older 421452-9800-000. Has anyone experienced similar problems and solved the issue? On another note, is the CO2 in the Oxygen/CO2 mixture essential for these experiments (tracking and interaction of lymphocytes in lymph nodes)? > > best wishes > > Andreas Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler PA 16002 V724-282-7145 F724-282-0745 Toll Free 877 lIVE-CELL (548-3235) [hidden email] |
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0000001ed7f52e4a-dmarc-request |
Re: objective catches bubbles during tissue imaging "commercial response"
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dan, It is an upright microscope with a large incubation chamber surrounding the stage and objective, heated to 37 C. We have a stage insert which has a metal plate, so that we can mount magnetic holders for plastic tubing. The dish is open and cell culture medium is pumped from a bottle into the dish and with another pump out of the dish back into the bottle. The oxygen is added to the medium before it reaches the dish using a T connector in the tubing. There are no condensation problems. I have no picture right now but will take one next time we run the experiment. It would be better to use a larger dish as the objective is quite big (35 mm diameter) and add the medium a bit further away, but then we don't have much space to place the magnetic holders. best wishes Andreas -----Original Message----- From: Dan Focht <[hidden email]> To: CONFOCALMICROSCOPY <[hidden email]> Sent: Fri, 15 Jul 2016 13:32 Subject: Re: objective catches bubbles during tissue imaging "commercial response" ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Andreas Is this an upright or inverted scope? You said the specimen is on a dish but is it in a closed or open type system? Are you dealing with condensation also? Would you send me a picture of your setup? and/or You can call Bioptechs and ask for Dan. Dan > On Jul 15, 2016, at 4:22 AM, [hidden email] wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Dear all, > > we have a strange problem with a Zeiss 20x NA 1.0 objective catching oxygen bubbles during live tissue imaging. We were doing these experiments sucessfully since many years on an Olympus setup, purging the medium with oxygen/5%CO2 close to the tissue (extracted lymph node) which is glued onto a 30 mm dish and kept at 37 degrees. The problem seems to be that the little oxygen bubbles are caught by the concave front lens of the Zeiss objective, unsurpringly resulting in a dramatic loss of signal. The Olympus 25x NA 1.05 lens did have a larger, flat front lens and we did not have this problem. I tried bubbling the oxygen into the bottle with the medium which is a bit further away, but was not able to keep the tissue alive during these conditions, the cells stop moving. There are several of these objectives from Zeiss and our Zeiss rep could not tell me if there are some with flat front lenses, we have an older 421452-9800-000. Has anyone experienced similar problems and solved the issue? On another note, is the CO2 in the Oxygen/CO2 mixture essential for these experiments (tracking and interaction of lymphocytes in lymph nodes)? > > best wishes > > Andreas Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler PA 16002 V724-282-7145 F724-282-0745 Toll Free 877 lIVE-CELL (548-3235) [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Andreas Thank you for the reply. This information leads me to ask the working distance of the lens. When you say Oxygen bubbles being caught by the lens do you mean micro-splatter from the bubbles that emerge or out-gas when media enters the warm dish or do you mean you are seeing the affect of the refractive index of the presence of Oxygen gas? Also I don't know if this would help but we make an Atmospheric Control Barrier Ring [ACBR] for upright lenses and dipping lenses that will retain the gas environment above the specimen in a dish. There is also a perfusion adapter to introduce and remove media while still isolating ambient room atmosphere. We can talk about that later. Dan On Jul 15, 2016, at 3:17 PM, [hidden email] wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dan, It is an upright microscope with a large incubation chamber surrounding the stage and objective, heated to 37 C. We have a stage insert which has a metal plate, so that we can mount magnetic holders for plastic tubing. The dish is open and cell culture medium is pumped from a bottle into the dish and with another pump out of the dish back into the bottle. The oxygen is added to the medium before it reaches the dish using a T connector in the tubing. There are no condensation problems. I have no picture right now but will take one next time we run the experiment. It would be better to use a larger dish as the objective is quite big (35 mm diameter) and add the medium a bit further away, but then we don't have much space to place the magnetic holders. best wishes Andreas -----Original Message----- From: Dan Focht <[hidden email]> To: CONFOCALMICROSCOPY <[hidden email]> Sent: Fri, 15 Jul 2016 13:32 Subject: Re: objective catches bubbles during tissue imaging "commercial response" ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Andreas Is this an upright or inverted scope? You said the specimen is on a dish but is it in a closed or open type system? Are you dealing with condensation also? Would you send me a picture of your setup? and/or You can call Bioptechs and ask for Dan. Dan > On Jul 15, 2016, at 4:22 AM, [hidden email] wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Dear all, > > we have a strange problem with a Zeiss 20x NA 1.0 objective catching oxygen bubbles during live tissue imaging. We were doing these experiments sucessfully since many years on an Olympus setup, purging the medium with oxygen/5%CO2 close to the tissue (extracted lymph node) which is glued onto a 30 mm dish and kept at 37 degrees. The problem seems to be that the little oxygen bubbles are caught by the concave front lens of the Zeiss objective, unsurpringly resulting in a dramatic loss of signal. The Olympus 25x NA 1.05 lens did have a larger, flat front lens and we did not have this problem. I tried bubbling the oxygen into the bottle with the medium which is a bit further away, but was not able to keep the tissue alive during these conditions, the cells stop moving. There are several of these objectives from Zeiss and our Zeiss rep could not tell me if there are some with flat front lenses, we have an older 421452-9800-000. Has anyone experienced similar problems and solved the issue? On another note, is the CO2 in the Oxygen/CO2 mixture essential for these experiments (tracking and interaction of lymphocytes in lymph nodes)? > > best wishes > > Andreas Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler PA 16002 V724-282-7145 F724-282-0745 Toll Free 877 lIVE-CELL (548-3235) [hidden email] Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler, PA 16002 www.bioptechs.com P: (724)282-7145 F: (724)282-0745 [hidden email] |
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