Leica TCS SP2 File Creation Time Stamp Question

[Ethan Cohen]

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I have resuscitated a Leica TCS SP2 confocal for physiology use. Aligned the lasers, and made it an upright unit on a DM-RE scope.

SP2s, like all Leica products are a little quirky

We are taking time-lapse z-series on it of live tissue.   We did about 4 hours of imaging..but I cannot find any time information of when files were created.
It seems you save a whole bunch at the end of the imaging session.


1.       Should we be using the xyzt format?

2.       Save after about a 1000MB data?

3.       Where is the time stamp of the files in the .lei or .tif files?  How to deal with this?


Ethan.


Ethan Cohen, Ph.D.
FDA Center for Devices and Radiological Health
Office: Rm 1204 WO62
Lab: Rm 1241 WO62
White Oak Federal Res Ctr.
10903 New Hampshire Ave.
Silver Spring, MD 20993
________________________________

[Rosemary.White]

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On SP2, time stamp is in the .txt file under TIME INFORMATION
Should show the time each image is saved in RAM.

good luck,
cheers,
Rosemary

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E [hidden email]


On 24/01/14 12:15 PM, "Cohen, Ethan D" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>I have resuscitated a Leica TCS SP2 confocal for physiology use. Aligned
>the lasers, and made it an upright unit on a DM-RE scope.
>
>SP2s, like all Leica products are a little quirky
>
>We are taking time-lapse z-series on it of live tissue.   We did about 4
>hours of imaging..but I cannot find any time information of when files
>were created.
>It seems you save a whole bunch at the end of the imaging session.
>
>
>1.       Should we be using the xyzt format?
>
>2.       Save after about a 1000MB data?
>
>3.       Where is the time stamp of the files in the .lei or .tif files?
>How to deal with this?
>
>
>Ethan.
>
>
>Ethan Cohen, Ph.D.
>FDA Center for Devices and Radiological Health
>Office: Rm 1204 WO62
>Lab: Rm 1241 WO62
>White Oak Federal Res Ctr.
>10903 New Hampshire Ave.
>Silver Spring, MD 20993
>________________________________